Integrating bioelectronics with cell-based synthetic biology
Nature Reviews Bioengineering,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 7, 2025
Язык: Английский
Compact transcription factor cassettes generate functional, engraftable motor neurons by direct conversion
Cell Systems,
Год журнала:
2025,
Номер
unknown, С. 101206 - 101206
Опубликована: Март 1, 2025
Язык: Английский
STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 17, 2024
Abstract
Targeting
DNA
payloads
into
human
(h)iPSCs
involves
multiple
time-consuming,
inefficient
steps
that
must
be
repeated
for
each
construct.
Here,
we
present
STRAIGHT-IN
Dual,
which
enables
simultaneous,
allele-specific,
single-copy
integration
of
two
with
100%
efficiency
within
one
week.
Notably,
Dual
leverages
the
platform
to
allow
near-scarless
cargo
integration,
facilitating
recycling
components
subsequent
cellular
modifications.
Using
investigated
how
promoter
choice
and
gene
syntax
influences
transgene
silencing,
demonstrate
impact
these
design
features
on
forward
programming
hiPSCs
neurons.
Furthermore,
designed
a
grazoprevir-inducible
synZiFTR
system
complement
widely-used
tetracycline-inducible
system,
providing
independent,
tunable,
temporally
controlled
expression
both
transcription
factors
functional
reporters.
The
unprecedented
speed
generates
homogenous
genetically
engineered
hiPSC
populations
represents
major
advancement
synthetic
biology
in
stem
cell
applications
opens
opportunities
precision
engineering.
Язык: Английский
Designer mammalian living materials through genetic engineering
Bioactive Materials,
Год журнала:
2025,
Номер
48, С. 135 - 148
Опубликована: Фев. 15, 2025
Язык: Английский
Model-guided design of microRNA-based gene circuits supports precise dosage of transgenic cargoes into diverse primary cells
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 25, 2024
Abstract
To
realize
the
potential
of
engineered
cells
in
therapeutic
applications,
transgenes
must
be
expressed
within
window
efficacy.
Differences
copy
number
and
other
sources
extrinsic
noise
generate
variance
transgene
expression
limit
performance
synthetic
gene
circuits.
In
a
context,
supraphysiological
can
compromise
phenotypes
lead
to
toxicity.
ensure
narrow
range
expression,
we
design
characterize
Co
mpact
m
icroRNA-
M
ediated
A
ttenuator
N
oise
D
osage
(
ComMAND
),
single-transcript,
microRNA-based
incoherent
feedforward
loop.
We
experimentally
tune
output
profile,
model
system
explore
additional
tuning
strategies.
By
comparing
two-gene
implementations,
highlight
precise
control
afforded
by
single-transcript
architecture,
particularly
at
relatively
low
numbers.
show
that
tightly
regulates
from
lentiviruses
precisely
controls
primary
human
T
cells,
rat
neurons,
mouse
embryonic
fibroblasts,
induced
pluripotent
stem
cells.
Finally,
effectively
sets
levels
clinically
relevant
FMRP1
FXN
window.
Together,
is
compact
tool
well-suited
specify
cargoes.
Язык: Английский