Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 2, 2025
Язык: Английский
Nature, Год журнала: 2024, Номер 632(8027), С. 1124 - 1130
Опубликована: Июль 24, 2024
Язык: Английский
Процитировано
18
Nature Reviews Microbiology, Год журнала: 2024, Номер 22(9), С. 572 - 586
Опубликована: Май 29, 2024
Язык: Английский
Процитировано
17
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Фев. 29, 2024
Abstract Methane is a potent greenhouse gas contributing to global warming. Microorganisms largely drive the biogeochemical cycling of methane, yet little known about viral contributions methane metabolism (MM). We analyzed 982 publicly available metagenomes from host-associated and environmental habitats containing microbial MM genes, expanding auxiliary metabolic genes (AMGs) three 24, including seven exclusive pathways. These AMGs are recovered on 911 contigs predicted infect 14 prokaryotic phyla Halobacteriota, Methanobacteriota, Thermoproteota. Of those most were encoded by viruses rumen (16/24), with substantially fewer (0–7/24). To search for additional an habitat, we generate methane-rich sediments in Vrana Lake, Croatia. Therein, find diverse communities, methanogens methanotrophs some encoding 13 that can modulate host metabolisms. However, none these directly participate Together findings suggest extent which use processes (e.g., MM) varies depending ecological properties habitat they dwell not always predictable properties.
Язык: Английский
Процитировано
16
Nature Reviews Gastroenterology & Hepatology, Год журнала: 2022, Номер 19(12), С. 805 - 813
Опубликована: Сен. 1, 2022
Язык: Английский
Процитировано
69
Nature Microbiology, Год журнала: 2023, Номер 8(11), С. 2170 - 2182
Опубликована: Сен. 25, 2023
Язык: Английский
Процитировано
27
Nature, Год журнала: 2024, Номер 632(8027), С. 1118 - 1123
Опубликована: Июль 24, 2024
Язык: Английский
Процитировано
15
Nature, Год журнала: 2024, Номер 632(8027), С. 1131 - 1136
Опубликована: Июль 24, 2024
Язык: Английский
Процитировано
11
The ISME Journal, Год журнала: 2024, Номер 18(1)
Опубликована: Янв. 1, 2024
Abstract Hadarchaeota inhabit subsurface and hydrothermally heated environments, but previous to this study, they had not been cultured. Based on metagenome-assembled genomes, most are heterotrophs that grow sugars amino acids, or oxidize carbon monoxide reduce nitrite ammonium. A few other genomes encode alkyl-coenzyme M reductases (Acrs), β-oxidation, Wood-Ljungdahl pathways, pointing toward multicarbon alkane metabolism. To identify the organisms involved in thermophilic oil degradation, we established anaerobic sulfate-reducing hexadecane-degrading cultures from sediments of Guaymas Basin. Cultures at 70°C were enriched one Hadarchaeon propose as Candidatus Cerberiarchaeum oleivorans. Genomic chemical analyses indicate Ca. C. oleivorans uses an Acr activate hexadecane hexadecyl-coenzyme M. β-oxidation pathway a tetrahydromethanopterin methyl branch Wood–Ljungdahl (mWL) allow complete oxidation CO2. Our results suggest syntrophic lifestyle with sulfate reducers, lacks respiration pathway. Comparative genomics show Acr, mWL, restricted family Hadarchaeota, which Cerberiarchaeaceae. Phylogenetic further mWL is basal all Hadarchaeota. By contrast, dehydrogenase/acetyl-coenzyme synthase complex Cerberiarchaeaceae was horizontally acquired Bathyarchaeia. The genes highly similar those alkane-oxidizing archaea such Methanoliparia Helarchaeales. support use Acrs degradation petroleum alkanes role oil-rich environments.
Язык: Английский
Процитировано
8
Science Advances, Год журнала: 2022, Номер 8(44)
Опубликована: Ноя. 4, 2022
Anaerobic methane metabolism is among the hallmarks of Archaea, originating very early in their evolution. Here, we show that ancestor metabolizers was an autotrophic CO2-reducing hydrogenotrophic methanogen possessed two main complexes, methyl-CoM reductase (Mcr) and tetrahydromethanopterin-CoM methyltransferase (Mtr), anaplerotic hydrogenases Eha Ehb, a set other genes collectively called "methanogenesis markers" but could not oxidize alkanes. Overturning recent inferences, demonstrate methyl-dependent methanogenesis has emerged multiple times independently, either due to loss Mtr while Mcr inherited vertically or from ancient lateral acquisition Mcr. Even if lost, Mtr, Eha, markers can persist, resulting mixotrophic metabolisms centered around Wood-Ljungdahl pathway. Through remnants, Thorarchaeia newly reconstructed order-level lineages Archaeoglobi Bathyarchaeia act as metabolically versatile players carbon cycling anoxic environments across globe.
Язык: Английский
Процитировано
35
ISME Communications, Год журнала: 2023, Номер 3(1)
Опубликована: Март 22, 2023
Abstract Metagenomic studies on geothermal environments have been central in recent discoveries the diversity of archaeal methane and alkane metabolism. Here, we investigated methanogenic populations inhabiting terrestrial features Yellowstone National Park (YNP) by combining amplicon sequencing with metagenomics mesocosm experiments. Detection methyl-coenzyme M reductase subunit A (mcrA) gene amplicons demonstrated a wide Mcr-encoding archaea inhabit differing physicochemical regimes across YNP. From three selected hot springs recovered twelve metagenome assembled genomes (MAGs) affiliated lineages cultured methanogens as well Candidatus (Ca.) Methanomethylicia, Ca. Hadesarchaeia, Archaeoglobi. These MAGs encoded potential for hydrogenotrophic, aceticlastic, hydrogen-dependent methylotrophic methanogenesis, or anaerobic short-chain oxidation. While represent minor fractions microbial community springs, experiments precursors resulted stimulation activity enrichment Methanosaeta Methanothermobacter uncultured including Korarchaeia, Nezhaarchaeia, We revealed that diverse metabolic to produce from different persist YNP can be enriched under conditions. This study highlights importance environmental laboratory-based expand our understanding their impact carbon transformations beyond.
Язык: Английский
Процитировано
19