A Facile LC-MS Method for Profiling Cholesterol and Cholesteryl Esters in Mammalian Cells and Tissues

Biochemistry, Год журнала: 2024, Номер 63(18), С. 2300 - 2309

Опубликована: Июль 10, 2024

Cholesterol is central to mammalian lipid metabolism and serves many critical functions in the regulation of diverse physiological processes. Dysregulation cholesterol causally linked numerous human diseases, therefore, vivo, concentrations flux cholesteryl esters (fatty acid cholesterol) are tightly regulated. While mass spectrometry has been an analytical method choice for detecting biological samples, hydrophobicity, chemically inert nature, poor ionization these neutral lipids have often proved a challenge developing lipidomics compatible liquid chromatography-mass (LC-MS) methods study them. To overcome this problem, here, we report reverse-phase LC-MS that with existing high-throughput strategies capable identifying quantifying from cells tissues. Using sensitive yet robust method, profiled different cell lines tissues provide comprehensive picture content Specifically, among esters, find largely possess monounsaturated polyunsaturated variants. Taken together, our class opens new avenues understanding systemic tissue-level under various conditions.

Язык: Английский

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Inhibition of liver cholesterol synthesis by a diet-induced gut hormone

Acta Pharmaceutica Sinica B, Год журнала: 2024, Номер 14(10), С. 4625 - 4627

Опубликована: Авг. 5, 2024

Язык: Английский

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Strategic Design of Tryptophan–Aspartic Acid-Containing Peptide Inhibitors Using Coronin 1 as a Template: Inhibition of Fusion by Enhancing Acyl Chain Order

The Journal of Physical Chemistry B, Год журнала: 2024, Номер unknown

Опубликована: Сен. 13, 2024

Enveloped viruses enter the host cell by fusing at membrane or entering via endocytosis and endosome. Conventional inhibitors target viral fusion protein to inactivate it for inducing fusion. These target-specific vis-à-vis virus-specific fail display their inhibitory efficacy against emerging remerging infections. This necessitates need develop broad-spectrum entry that are effective irrespective of virus. Using a broad range targeting techniques, can modify physical characteristics membrane, making less prone We have previously shown two tryptophan-aspartic acid (WD)-containing hydrophobic peptides, TG-23 GG-21, from coronin 1, phagosomal protein, inhibit modulating organization dynamics. In present work, we designed WD-containing hydrophilic QG-22 AG-22, using 1 as template evaluated efficacies in absence presence cholesterol. Our results demonstrate AG-22 concentration measurements depth-dependent dynamics reveal they impede enhancing acyl chain order. Overall, our validate hypothesis designing membrane's properties. addition, demonstrates hydrophobicity might not be critical determinant development peptide-based inhibitors.

Язык: Английский

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Calycosin-7-O-β-D-Glucoside Ameliorates Palmitate-Induced Lipid Accumulation in HT22 Cells

Actas Españolas de Psiquiatría, Год журнала: 2024, Номер 52(5), С. 641 - 652

Опубликована: Окт. 5, 2024

The pathogenesis of Alzheimer's disease (AD) is complex. Recent research suggests that AD patients have early disorders in brain cholesterol metabolism. Cholesterol and its derivatives accumulate neurons, leading to p-Tau overproduction synaptic dysfunction, initiating progression. Calycosin-7-O-β-D-glucoside (CG), a distinctive constituent Astragali Radix, holds representative position. Many clinical trials demonstrated CG can attenuate cerebral ischemia/reperfusion injury preserve the structural integrity blood-brain barrier. However, whether alleviates tau-mediated neurodegeneration by increasing efflux after lipid accumulation remains unexplored.

Язык: Английский

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How can early stress influence later Alzheimer risk? Possible mediators and underlying mechanisms.

Biological Psychiatry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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