Biomolecules, Год журнала: 2024, Номер 14(12), С. 1570 - 1570
Опубликована: Дек. 9, 2024
The polyamines putrescine, spermidine, and spermine are polycations ubiquitously present in cells, where they exert pleiotropic functions cellular mechanisms like proliferation, protein synthesis (through the hypusination of transcription factor EIF5a), redox balance, autophagy, different forms cell death [...]
Язык: Английский
Journal of the American Heart Association, Год журнала: 2025, Номер unknown
Опубликована: Янв. 16, 2025
Polyamines have been suggested to play pivotal roles in ischemic stroke and neurodegenerative disorders, but the associations of plasma polyamines with poststroke cognitive impairment (PSCI) remain unclear. We aimed prospectively investigate putrescine, spermidine, spermine PSCI among patients a multicenter cohort study. measured polyamine levels at baseline 619 from preplanned ancillary study CATIS (China Antihypertensive Trial Acute Ischemic Stroke). used Mini-Mental State Examination Montreal Cognitive Assessment evaluate function 3-month follow-up after stroke, was defined as score <27 or <25. According score, were positively associated PSCI. The adjusted odds ratios for highest versus lowest quartile 1.81 (95% CI, 1.09-3.00), 1.09-3.01), 1.92 1.15-3.20), respectively. In addition, putrescine (net reclassification improvement, 32.08%; P<0.001; integrated discrimination 1.62%; P=0.002), spermidine 25.29%; P=0.002; 1.22%; P=0.006), 16.54%; P=0.045; 1.36%; P=0.004) could significantly improve risk beyond established factors. There similar significant relationships when by score. Higher increased stroke. Our findings suggest that should be implicated pathophysiologic processes may potential intervention targets URL: https://www.clinicaltrials.gov; Unique identifier: NCT01840072.
Язык: Английский
Процитировано
1
Molecules, Год журнала: 2025, Номер 30(8), С. 1666 - 1666
Опубликована: Апрель 8, 2025
Microalgae are microscopic unicellular organisms that inhabit marine, freshwater, and moist terrestrial ecosystems. The vast number diversity of microalgal species provide a significant reservoir biologically active compounds, highly promising for biomedical applications. Diatoms eukaryotic algae belonging to the class Bacillariophyceae. They possess intricately structured silica-based cell walls, which contain long-chain polyamines play important roles in formation silica. Long-chain uncommon found only produce biosilica. Diatomite, is marine sediment remains silica skeleton diatoms, could be an abundant source biogenic can easily converted particles. This concise review focuses on biofabrication polyamine-based nanosilica from diatoms highlights possibility utilizing diatom biosilica as nanocarrier drug siRNA delivery, bioimaging, bone tissue engineering. challenges may affect production, including environmental stresses climate change, discussed together with prospect increasing diatom-based production desired nanostructures via genetic manipulation.
Язык: Английский
Процитировано
1
Applied Sciences, Год журнала: 2025, Номер 15(2), С. 659 - 659
Опубликована: Янв. 11, 2025
Enteral nutrition (EN) formulas are necessary for critically ill patients to meet their metabolic requirements. Polyamines (putrescine, spermidine, and spermine) crucial dietary components, with spermidine being particularly interesting due its multiple proposed benefits. The requirements intake of polyamines have yet be investigated in adult hospitalised intensive care units (ICUs) who exclusively fed via commercial EN formulas. aim this study was determine the polyamine content other biogenic amines total gastric residual volume (GRV) ICU during first seven days hospitalisation. were analysed 16 using high-performance liquid chromatography (HPLC). clinical data eight both sexes aged 47 77 admitted analysed. Differences existed among N-acetyl putrescine higher than that remaining amines. daily less 100 μmol (the is above 400 μmol). An inverse correlation between GRV, without effects from Polyamine receiving low could impact these patients’ feeding tolerance. These findings underscore need further research explore implications increasing
Язык: Английский
Процитировано
0
Molecules, Год журнала: 2025, Номер 30(4), С. 955 - 955
Опубликована: Фев. 19, 2025
Spermidine is a natural autophagy-inducer and anti-aging compound. Herein, we investigated potential autophagy-independent mechanism of spermidine, namely its capability to directly impede LDL oxidation, an early step in atherogenesis. In our vitro-model, oxidation was induced by the addition CuCl2 presence increasing concentrations degree lipid, as well protein part LDL, measured. We found that spermidine concentration-dependently inhibited production lipid hydroperoxides, malondialdehyde, oxidation-specific immune epitopes particle, associated with decreased relative electrophoretic mobilities, respectively. For example, LPO content significantly lower when oxidized 500 µg/mL (26.9 ± 1.6 nmol/mg LDL) than absence (180.6 7.7 p < 0.0001). When oxLDL obtained under concentrations, cytotoxicity EA.hy926 cells decreased. Quantum chemical calculations show reaction between hydroxyl radicals exergonic. conclude direct inhibitor due scavenge radicals. Thus, supplementation might be suitable tool atherogenesis (cardio)vascular diseases. Further prospective clinical studies are needed evaluate atheroprotective/health-promoting effects spermidine-rich diets.
Язык: Английский
Процитировано
0
Methods in enzymology on CD-ROM/Methods in enzymology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Toxicology in Vitro, Год журнала: 2025, Номер unknown, С. 106048 - 106048
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Journal of the American Chemical Society, Год журнала: 2025, Номер unknown
Опубликована: Март 31, 2025
The biosynthetic pathways of natural products involve unusual biochemical reactions catalyzed by unique enzymes. Aminopropylation, although apparently simple, is an extremely rare modification outside polyamine biosynthesis. canonical pathway used in the biosynthesis peptide-adenylate antibiotic microcin C E. coli (Eco-McC) entails alkylation S-adenosyl-methionine-derived 3-amino-3-carboxypropyl group adenylate moiety and subsequent decarboxylation to yield bioactive aminopropylated compound. Here, we report structure a new member family antibiotics, Bsu-McC, produced Bacillus subtilis MG27, which employs alternative aminopropylation pathway. Like Eco-McC, Bsu-McC consists peptide that facilitates prodrug import into susceptible bacteria warhead, nonhydrolyzable modified isoasparaginyl-adenylate, which, when released cytoplasm, binds aspartyl-tRNA synthetase (AspRS) inhibiting translation. In contrast whose warhead carries single aminopropyl attached phosphate decorated with tandem two groups. Our silico docking AspRS-tRNA complex suggests groups form extended interactions enzyme tRNA, stabilizing enzyme–inhibitor complex. We show results 32-fold increase biological activity peptidyl-adenylate. also B. adopted for homologous 3-aminopropyltransferases utilize decarboxylated S-adenosylmethionine as substrate. Additionally, alters social behavior producer strain, resulting sharp decrease their ability biofilms.
Язык: Английский
Процитировано
0
mBio, Год журнала: 2025, Номер unknown
Опубликована: Март 31, 2025
ABSTRACT Acinetobacter baumannii is a gram-negative, opportunistic pathogen frequently associated with hospital-acquired infections. Due to its resistance multiple antibiotics, it emerging as major nosocomial pathogen, causing wide range of severe infections such pneumonia, meningitis, and bloodstream In many cases, the intrinsic activities efflux pumps contribute development drug resistance. The polyamine-binding protein AmvR regulates multidrug pump AmvA, which pivotal for transporting polyamines, an abundant prevalent class amino acid-derived metabolites. Here, we report crystal structure bound physiological substrate, spermidine, thereby offering structural functional insights into AmvR. By employing electrophoretic mobility shift assays DNase I footprinting, identified recognition sites intragenic regions amvR amvA by Moreover, fluorescence reporter assay revealed that repressed expressions AmvA addition, isothermal titration calorimetry indicated spermidine may be natural ligand Collectively, these experiments provided better understanding substrate discovery potential inhibitors. Furthermore, our results binding triggers localized conformational change in protein, supported size-exclusion chromatography static light scattering, suggesting distinctive regulatory mechanism within TetR family transcription factors. IMPORTANCE Multidrug are key contributors clinically significant various gram-negative pathogens responsible These often possess genes encode pumps. Identifying specific proteins control expression pumps, along elucidating mechanisms triggered effectors, presents complex challenge. this study, have resolved structures both unbound spermidine-bound states. To best knowledge, represents first validated model polyamine-bound transcriptional regulator. Through detailed analysis assays, pinpointed critical residues recognition, providing foundation future
Язык: Английский
Процитировано
0
Biomolecules, Год журнала: 2025, Номер 15(4), С. 539 - 539
Опубликована: Апрель 7, 2025
Autism spectrum disorder (ASD) is a genetically heterogeneous syndrome characterized by repetitive, restricted, and stereotyped behaviors, along with persistent difficulties social interaction communication. Despite its increasing prevalence globally, the underlying pathogenic mechanisms of this complex neurodevelopmental remain poorly understood. Therefore, identification reliable biomarkers could play crucial role in enabling early screening more precise classification ASD subtypes, offering valuable insights into physiopathology aiding customization treatment or interventions. Biogenic amines, including serotonin, histamine, dopamine, epinephrine, norepinephrine, polyamines, are class organic compounds mainly produced decarboxylation amino acids. A substantial portion genetic variation observed has been linked to genes that either directly indirectly involved metabolism biogenic amines. Their potential involvement become an area growing interest due their pleiotropic activities central nervous system, where they act as both neurotransmitters neuromodulators hormones. This review examines amines ASD, particular focus on alterations enzymes responsible for synthesis degradation.
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 29, 2025
Язык: Английский
Процитировано
0