Osteoblast‐Derived Mitochondria Formulated with Cationic Liposome Guide Mesenchymal Stem Cells into Osteogenic Differentiation

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

Abstract While mitochondria are known to be essential for intracellular energy production and overall function, emerging evidence highlights their role in influencing cell behavior through mitochondrial transfer. This phenomenon provides a potential basis the development of treatment strategies tissue damage degeneration. study aims evaluate whether isolated from osteoblasts can promote osteogenic differentiation mesenchymal stem cells (MSCs). Mitochondria MSCs, which primarily utilize glycolysis, compared with those MG63 cells, depend on oxidative phosphorylation. both types then encapsulated cationic liposomes transferred impact is assessed. delivery MSCs grown two‐ three‐dimensional cultures results increased expression markers, including Runt‐related transcription factor 2, Osterix, Osteopontin, upregulation genes involved Bone morphogenetic protein 2 signaling calcium import. accompanied by influx regulated Wnt/β‐catenin pathway. Transplantation spheroids containing MG63‐derived bone defect animal models improves regeneration. The suggest that effectively guides toward osteogenesis, paving way mitochondria‐transplantation therapies.

Язык: Английский

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Targeting mitochondrial transfer as a promising therapeutic strategy

Trends in Molecular Medicine, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

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Role of mitochondrial dysfunction in the pathogenesis of rheumatoid arthritis: Looking closely at fibroblast- like synoviocytes

Mitochondrion, Год журнала: 2023, Номер 73, С. 62 - 71

Опубликована: Окт. 30, 2023

Язык: Английский

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Molecular and cellular mechanisms of mitochondria transfer in models of central nervous system disease

Journal of Cerebral Blood Flow & Metabolism, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 14, 2024

In the central nervous system (CNS), neuronal function and dysfunction are critically dependent on mitochondrial integrity activity. damaged or diseased brains, reduces adenosine triphosphate (ATP) levels impairs ATP-dependent neural firing neurotransmitter dynamics. Restoring capacity to generate ATP may be fundamental in restoring function. Recent studies animals humans have demonstrated that endogenous mitochondria released into extracellular environment transported exchanged between cells CNS. Under pathological conditions CNS, intercellular transfer contributes new classes of signaling multifunctional cellular activities, thereby triggering deleterious effects promoting beneficial responses. Therefore, take full advantage mitochondria, it useful transplant healthy viable tissues. this review, we describe recent findings mechanisms provide an overview experimental methodologies, including tissue sourcing, isolation, storage, modification, aimed at optimizing transplantation therapy for CNS disorders. Additionally, examine clinical relevance potential strategies therapeutic application transplantation.

Язык: Английский

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Autologous mitochondrial transplantation in male mice as a strategy to prevent deleterious effects of peripheral ischemia-reperfusion

AJP Cell Physiology, Год журнала: 2023, Номер 326(2), С. C449 - C456

Опубликована: Дек. 25, 2023

Ischemia-reperfusion (IR) is known to induce severe tissue damage, notably through mitochondrial dysfunction. Mitochondrial transplantation has emerged as a promising therapeutic strategy in cardiac IR; however, few studies have previously assessed its efficacy the context of peripheral IR. Therefore, objective this study was assess effect hindlimb model IR injury. Thirty-six SWISS mice were divided into three groups: control (CTL,

Язык: Английский

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Mitochondrial transfer and implications for muscle function in idiopathic inflammatory myopathies

Clinical and Experimental Rheumatology, Год журнала: 2023, Номер unknown

Опубликована: Окт. 15, 2023

Язык: Английский

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Mitochondrial Transplantation: Adaptive Bio-enhancement

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 23, 2024

Abstract Mitochondria, known as the powerhouse of cell, are essential for cellular energy production. Dysfunction in mitochondrial function can significantly affect various organs. Transplanting healthy mitochondria enhance bioenergetics diseased cells and treat conditions, yet limits transplantation still unknown. Our study reveals that source transplanted is not restricted by species, recipient show no significant immune response to from different lineages. We also found metabolic compatibility between exogenous crucial, transplanting species endow with distinct characteristics combat diseases. Furthermore, our data indicate there competition among varying functions, more powerful yielding better therapeutic effects. Notably, we have an upper limit bio-enhancement provided mitochondria. research proposes a feasible path human through transplantation—adaptive bio-enhancement. Mitochondrial highly clinically valuable technique. I further expanded variety sources demonstrated potential adaptively biological functions.

Язык: Английский

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Five Padua days on muscle and mobility medicine (2024Pdm3) 27 February - 2 March, 2024 at Hotel Petrarca, Thermae of Euganean Hills, Padua, and San Luca Hall, Prato della Valle, Padua, Italy

European Journal of Translational Myology, Год журнала: 2023, Номер unknown

Опубликована: Дек. 18, 2023

At the end of 2023 Padua Days Muscle and Mobility Medicine next year's meeting was scheduled from 27 February to 2 March 2024 (2024Pdm3). During summer autumn program confirmed with Scientific Sessions that will take place over five days, starting in afternoon 27, at Conference Room Hotel Petrarca, Thermae Euganean Hills (Padua), Italy. As usual, day be spent Padua, this occasion San Luca Hall Santa Giustina monastery Prato della Valle, Collected during Autumn 2023, many more titles abstracts than expected were submitted, forcing organization parallel sessions both on 1 confirming attractiveness Pdm3. The days include oral presentations scientists clinicians Argentina, Austria, Belgium, Brazil, Canada, Denmark, Egypt, France, Germany, Iceland, Ireland, Italy, Romania, Russia, Slovenia, Switzerland, UK USA. Together preliminary Program December 1, early submitted Abstracts is e-published Issue 33 (4) European Journal Translational Myology (EJTM). You are invited join, submitting your Last Minute [email protected] by 2024. Furthermore, generous deadline May 20, 2024, submit please “Communications” (Clarivate’s ESCI Impact factor 2.2; SCOPUS Cite Score: 3.2). See you soon Petrarca Montegrotto Terme, but complete can followed home via zoom connection.

Язык: Английский

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