A primer on global molecular responses to exercise in skeletal muscle: Omics in focus

Journal of sport and health science/Journal of Sport and Health Science, Год журнала: 2025, Номер unknown, С. 101029 - 101029

Опубликована: Фев. 1, 2025

Advances in skeletal muscle omics has expanded our understanding of exercise-induced adaptations at the molecular level. Over past 2 decades, transcriptome studies have detailed acute and chronic responses to resistance, endurance, concurrent exercise, focusing on variables such as training status, nutrition, age, sex, metabolic health profile. Multi-omics approaches, integration transcriptomic epigenetic data, along with emerging ribosomal RNA sequencing advancements, further provided insights into how adapts exercise across lifespan. Downstream transcriptome, proteomic phosphoproteomic identified novel regulators adaptations, while single-cell/nucleus spatial technologies promise evolve cellular specialization communication around cells. This narrative review highlights (a) historical foundations muscle, (b) current research 3 layers cascade (DNA, RNA, protein), (c) applications single-cell study adaptation exercise. Further elaboration muscle's global footprint using multi-omics methods will help researchers practitioners develop more effective targeted approaches improve well athletic performance.

Язык: Английский

---

The utility—and limitations—of the rodent synergist ablation model in examining mechanisms of skeletal muscle hypertrophy

AJP Cell Physiology, Год журнала: 2024, Номер 327(3), С. C607 - C613

Опубликована: Июль 29, 2024

In this issue, Burke et al. discuss the utility of rodent synergist ablation (SA) model for examining mechanisms associated with skeletal muscle hypertrophy. invited perspective, we aim to complement their original perspective by discussing limitations along alternative mechanical overload models that have strengths and limitations.

Язык: Английский

---

microRNA-1 Regulates Metabolic Flexibility in Skeletal Muscle via Pyruvate Metabolism

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 10, 2024

Abstract MicroRNA-1 (miR-1) is the most abundant miRNA in adult skeletal muscle. To determine function of miR-1 muscle, we generated an inducible, muscle-specific knockout (KO) mouse. Integration RNA-sequencing (RNA-seq) data from KO muscle with Argonaute 2 enhanced crosslinking and immunoprecipitation sequencing (AGO2 eCLIP-seq) human identified target genes involved glycolysis pyruvate metabolism. The loss induced cancer-like metabolic reprogramming, as shown by higher kinase isozyme M2 (PKM2) protein levels, which promoted glycolysis. Comprehensive bioenergetic phenotyping combined proteomics metabolomics further demonstrated that inflexibility a result oxidation resistance. While genetic reduced endurance exercise performance mice C. elegans, physiological down-regulation expression response to hypertrophic stimulus both humans causes similar reprogramming supports cell growth. Taken together, these identify novel post-translational mechanism metabolism regulation mediated miR-1.

Язык: Английский

---

The risks and rewards of the synergist ablation model in skeletal muscle biology research

AJP Cell Physiology, Год журнала: 2024, Номер 327(3), С. C599 - C600

Опубликована: Июль 29, 2024

Язык: Английский

---