Urine metabolite changes after cardiac surgery predict acute kidney injury DOI Creative Commons
Qi Zeng,

Jinghan Feng,

Xinni Zhang

и другие.

Clinical Kidney Journal, Год журнала: 2024, Номер 17(8)

Опубликована: Июль 16, 2024

Acute kidney injury (AKI) is a serious complication in patients undergoing cardiac surgery, with the underlying mechanism remaining elusive and lack of specific biomarkers for surgery-associated AKI (CS-AKI). We performed an untargeted metabolomics analysis urine samples procured from cohort or without at 6 24 h following surgery. Based on differential urinary metabolites discovered, we further examined expressions key metabolic enzymes that regulate these during using mouse model ischemia-reperfusion (IRI) hypoxia-treated tubular epithelial cells (TECs). The metabolomic profiles were significantly different those non-AKI patients, including upregulation tryptophan metabolism- aerobic glycolysis-related metabolites, such as l-tryptophan d-glucose-1-phosphate, downregulation fatty acid oxidation (FAO) tricarboxylic (TCA) cycle-related metabolites. Spearman correlation showed serum creatinine was positively correlated indole, which had high accuracy predicting AKI. In animal experiments, demonstrated expression rate-limiting glycolysis, hexokinase II (HK2), upregulated renal IRI. However, TCA enzyme citrate synthase downregulated after vitro, hypoxia induced TECs. addition, FAO-related gene peroxisome proliferator-activated receptor alpha (PPARα) remarkably This study presents related to CS-AKI, indicating rewiring metabolism AKI, identifying potential biomarkers.

Язык: Английский

Kynurenine Pathway after Kidney Transplantation: Friend or Foe? DOI Open Access
Izabela Zakrocka, Ewa M. Urbańska, Wojciech Załuska

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 9940 - 9940

Опубликована: Сен. 14, 2024

Kidney transplantation significantly improves the survival of patients with end-stage kidney disease (ESKD) compared to other forms replacement therapy. However, transplant recipients’ outcomes are not fully satisfactory due increased risk cardiovascular diseases, infections, and malignancies. Immune-related complications remain biggest challenge in management graft recipients. Despite broad spectrum immunosuppressive agents available more detailed methods used monitor their effectiveness, chronic allograft nephropathy remains most common cause rejection. The kynurenine (KYN) pathway is main route tryptophan (Trp) degradation, resulting production a plethora substances ambiguous properties. Conversion Trp KYN by enzyme indoleamine 2,3-dioxygenase (IDO) rate-limiting step determining formation next from pathway. IDO activity, as well subsequent metabolites pathway, highly dependent on balance between pro- anti-inflammatory conditions. Moreover, products themselves possess immunomodulating properties, e.g., modify activity control immune-related processes. were widely studied neurological disorders but recently gained attention researchers context immune-mediated diseases. Evidence that this degradation may represent peripheral tolerogenic significant implications for further fueled interest. Our review aimed present recent knowledge about role pathogenesis, diagnosis, monitoring, treatment complications.

Язык: Английский

Процитировано

2

Depression, stress, and tryptophan metabolism through the kynurenine pathway: treatment strategies from the perspective of Chinese herbal medicine DOI
Wen Li, Lili Yang,

Haozhi Chen

и другие.

Metabolic Brain Disease, Год журнала: 2024, Номер 40(1)

Опубликована: Ноя. 15, 2024

Язык: Английский

Процитировано

2

Unraveling the nexus of NAD+ metabolism and diabetic kidney disease: insights from murine models and human data DOI Creative Commons
Sisi Yang, Weiyuan Gong, Yujia Wang

и другие.

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Май 21, 2024

Background Nicotinamide adenine dinucleotide (NAD+) is a critical coenzyme involved in kidney disease, yet its regulation diabetic disease (DKD) remains inadequately understood. Objective Therefore, we investigated the changes of NAD+ levels DKD and underlying mechanism. Methods Alternations biosynthesis enzymes were detected kidneys from streptozotocin-induced mouse model by real-time PCR immunoblot. The distribution de novo synthetic was explored via immunohistochemical study. metabolite measured LC-MS. Human data NephroSeq analyzed to verify our findings. Results study showed that decreased kidneys. Both mRNA protein kynurenine 3-monooxygenase (KMO) synthesis pathway decreased, while salvage consuming remained unchanged. Further analysis human suggested KMO, primarily expressed proximal tubules shown staining, consistently downregulated Conclusion Our demonstrated KMO might be responsible for reduction kidneys, offering valuable insights into complex regulatory mechanisms DKD.

Язык: Английский

Процитировано

1

Urine metabolite changes after cardiac surgery predict acute kidney injury DOI Creative Commons
Qi Zeng,

Jinghan Feng,

Xinni Zhang

и другие.

Clinical Kidney Journal, Год журнала: 2024, Номер 17(8)

Опубликована: Июль 16, 2024

Acute kidney injury (AKI) is a serious complication in patients undergoing cardiac surgery, with the underlying mechanism remaining elusive and lack of specific biomarkers for surgery-associated AKI (CS-AKI). We performed an untargeted metabolomics analysis urine samples procured from cohort or without at 6 24 h following surgery. Based on differential urinary metabolites discovered, we further examined expressions key metabolic enzymes that regulate these during using mouse model ischemia-reperfusion (IRI) hypoxia-treated tubular epithelial cells (TECs). The metabolomic profiles were significantly different those non-AKI patients, including upregulation tryptophan metabolism- aerobic glycolysis-related metabolites, such as l-tryptophan d-glucose-1-phosphate, downregulation fatty acid oxidation (FAO) tricarboxylic (TCA) cycle-related metabolites. Spearman correlation showed serum creatinine was positively correlated indole, which had high accuracy predicting AKI. In animal experiments, demonstrated expression rate-limiting glycolysis, hexokinase II (HK2), upregulated renal IRI. However, TCA enzyme citrate synthase downregulated after vitro, hypoxia induced TECs. addition, FAO-related gene peroxisome proliferator-activated receptor alpha (PPARα) remarkably This study presents related to CS-AKI, indicating rewiring metabolism AKI, identifying potential biomarkers.

Язык: Английский

Процитировано

0