The Kaohsiung Journal of Medical Sciences,
Год журнала:
2023,
Номер
39(11), С. 1096 - 1105
Опубликована: Авг. 14, 2023
To
investigate
the
regulatory
mechanisms
and
effects
of
LIM
SH3
protein
1
(LASP1)
on
osteoarthritis
(OA).
IL-1β
was
used
to
induce
OA
in
cell
models.
Viability
apoptosis
chondrocytes
were
assessed.
The
expressions
tumor
necrsis
factor-α
(TNF-α)
IL-6
measured
by
ELISA
kit,
Quantitative
reverse
transcription
polymerase
chain
reaction
(qRT-PCR)
Western
blot
performed
test
expression
related
proteins.
STRING
database
predict
relationship
between
LASP1
DNA
methyltransferase
(DNMT1).
tight
junction
2
(TJP2)
Gene
Expression
Omnibus
data
analyzed
for
differential
genes.
Methylation-specific
PCR
detected
methylation
TJP2
promoter
region,
chromatin
immunoprecipitation
enrichment
DNMT1
region.
Safranin
O-Fast
Green
staining
hematoxylin
eosin
determine
OARSI
score
evaluate
pathological
conditions
joint
tissues.
highly
expressed
IL-1β-induced
Silencing
promoted
chondrocyte
proliferation
Collagen
II
Aggrecan
inhibited
apoptosis,
inflammatory
factors,
matrix
metalloprotein
expression.
is
weakly
models,
promotes
region
interacting
with
DNMT1.
attenuated
degeneration
promoting
Similarly,
silencing
alleviate
articular
cartilage
injury
mice
OA.
DNMT1,
thereby
alleviating
damage
mice.
Journal of Translational Medicine,
Год журнала:
2024,
Номер
22(1)
Опубликована: Фев. 14, 2024
Abstract
Skeletal
system
disease
(SSD)
is
defined
as
a
class
of
chronic
disorders
skeletal
with
poor
prognosis
and
causes
heavy
economic
burden.
m6A,
methylation
at
the
N6
position
adenosine
in
RNA,
reversible
dynamic
modification
posttranscriptional
mRNA.
Evidences
suggest
that
m6A
modifications
play
crucial
role
regulating
biological
processes
all
kinds
diseases,
such
malignancy.
Recently
studies
have
revealed
most
abundant
epigentic
modification,
involved
progression
SSD.
However,
function
SSD
not
fully
illustrated.
Therefore,
make
clear
relationship
between
pathogenesis
might
provide
novel
sights
for
prevention
targeted
treatment
This
article
will
summarize
recent
advances
regulation
SSD,
including
osteoporosis,
osteosarcoma,
rheumatoid
arthritis
osteoarthritis,
discuss
potential
clinical
value,
research
challenge
future
prospect
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
170, С. 116066 - 116066
Опубликована: Дек. 28, 2023
Rheumatoid
arthritis
(RA)
is
a
multifaceted
autoimmune
disease
characterized
by
systemic
inflammation,
affecting
both
articular
and
extra-articular
structures.
This
condition
results
in
inflammation
of
joints
synovial
membranes,
accompanied
the
development
comorbidities.
Despite
extensive
research,
precise
pathogenic
mechanisms
responsible
for
RA
have
yet
to
be
completely
understood.
RNA
methylation,
burgeoning
epigenetic
alteration,
assumes
pivotal
function
regulation
myriad
biological
phenomena,
encompassing
immunity,
DNA
damage
response,
tumorigenesis,
metastasis,
stem
cell
renewal,
adipocyte
differentiation,
circadian
rhythms,
cellular
division.
The
N6-methyladenosine
(m6A)
modification
most
prevalent
among
various
modifications
found
mammalian
mRNA.
Recent
studies
provided
evidence
significant
role
played
m6A
pathophysiological
progression
RA.
review
aims
provide
comprehensive
analysis
progress
made
research
focused
on
context
RA,
consolidate
underlying
involved
during
initiation
discuss
potential
targeting
as
viable
therapeutic
approach
Cells,
Год журнала:
2024,
Номер
13(11), С. 915 - 915
Опубликована: Май 25, 2024
Rheumatoid
arthritis
(RA)
is
a
chronic
inflammatory
joint
disease
characterised
by
the
formation
of
hyperplastic
pannus,
as
well
cartilage
and
bone
damage.
The
pathogenesis
RA
complex
involves
broad
interactions
between
various
cells
present
in
inflamed
synovium,
including
fibroblast-like
synoviocytes
(FLSs),
macrophages,
T
cells,
among
others.
Under
conditions,
these
are
activated,
further
enhancing
responses
angiogenesis
promoting
degradation.
Novel
treatment
methods
for
greatly
needed,
mesenchymal
stromal
(MSCs)
have
been
suggested
promising
new
regenerative
immunomodulatory
treatment.
In
this
paper,
we
MSCs
RA-FLSs,
macrophages
summarise
studies
examining
use
preclinical
clinical
studies.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Июль 12, 2024
N
6
-methyladenosine
(m
A)
is
a
chemical
modification
of
RNA
and
has
become
widely
discussed
topic
among
scientific
researchers
in
recent
years.
It
distributed
various
organisms,
including
eukaryotes
bacteria.
been
found
that
m
A
composed
writers,
erasers
readers
involved
biological
functions
such
as
splicing,
transport
translation
RNA.
The
balance
the
human
immune
microenvironment
important
for
health
abnormalities.
Increasing
studies
have
affects
development
diseases
inflammatory
enteritis
systemic
lupus
erythematosus
(SLE)
by
participating
homeostatic
regulation
vivo
.
In
this
manuscript,
we
introduce
composition,
function,
its
progression
diseases,
providing
new
targets
directions
treatment
clinical
practice.
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Март 24, 2025
Dynamic
changes
in
various
forms
of
RNA
modification
are
critical
to
the
functional
homeostasis
immune
system
and
pathophysiology
autoimmune
diseases.
modification-related
proteins
play
an
essential
role
these
processes.
At
present,
research
methods
diseases
mainly
detect
expression
tissues
or
cells,
but
there
is
a
lack
explorations
target
RNAs
in-depth
mechanisms.
Considering
important
CD4
+
T
cell
dysfunction
pathogenesis
progression
diseases,
regulatory
effect
abnormal
on
cells
deserves
attention,
which
will
provide
perspective
for
further
exploring
mechanism
In
this
Review,
we
discuss
patients
with
highlight
effects
cells.
