Nicotinamide riboside activates renal metabolism and protects the kidney in a model of Alport syndrome DOI Open Access
Bryce A. Jones, Debora L. Gisch, Komuraiah Myakala

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 29, 2024

ABSTRACT Chronic kidney disease (CKD) is associated with renal metabolic disturbances, including impaired fatty acid oxidation (FAO). Nicotinamide adenine dinucleotide (NAD + ) a small molecule that participates in hundreds of metabolism-related reactions. NAD levels are decreased CKD, and supplementation protective. However, both the mechanism how protects from as well cell types involved, poorly understood. Using mouse model Alport syndrome, we show nicotinamide riboside (NR), an precursor, stimulates peroxisome proliferator-activated receptor alpha signaling restores FAO proximal tubules, thereby protecting CKD sexes. Bulk RNA-sequencing shows pathways mice activated by NR These transcriptional changes confirmed orthogonal imaging techniques biochemical assays. Single nuclei spatial transcriptomics, first their kind mice, tubule cells. Finally, also report, for time, sex differences at level this model. In summary, identify nephroprotective demonstrate cells substantially contribute to benefit.

Язык: Английский

NAD+ prevents chronic kidney disease by activating renal tubular metabolism DOI Creative Commons
Bryce A. Jones, Debora L. Gisch, Komuraiah Myakala

и другие.

JCI Insight, Год журнала: 2025, Номер 10(5)

Опубликована: Март 9, 2025

Chronic kidney disease (CKD) is associated with renal metabolic disturbances, including impaired fatty acid oxidation (FAO). Nicotinamide adenine dinucleotide (NAD+) a small molecule that participates in hundreds of metabolism-related reactions. NAD+ levels are decreased CKD, and supplementation protective. However, both the mechanism how protects from as well cell types involved, poorly understood. Using mouse model Alport syndrome, we show nicotinamide riboside (NR), an precursor, stimulated PPARα signaling restored FAO proximal tubules, thereby protecting CKD sexes. Bulk RNA-sequencing showed pathways were mice activated by NR These transcriptional changes confirmed orthogonal imaging techniques biochemical assays. Single-nuclei RNA sequencing spatial transcriptomics, first their kind to our knowledge mice, tubule cells. Finally, also report, for time knowledge, sex differences at level this model. In summary, data herein identify nephroprotective they demonstrate benefit localizes

Язык: Английский

Процитировано

0
Nicotinamide riboside activates renal metabolism and protects the kidney in a model of Alport syndrome DOI Open Access
Bryce A. Jones, Debora L. Gisch, Komuraiah Myakala

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 29, 2024

ABSTRACT Chronic kidney disease (CKD) is associated with renal metabolic disturbances, including impaired fatty acid oxidation (FAO). Nicotinamide adenine dinucleotide (NAD + ) a small molecule that participates in hundreds of metabolism-related reactions. NAD levels are decreased CKD, and supplementation protective. However, both the mechanism how protects from as well cell types involved, poorly understood. Using mouse model Alport syndrome, we show nicotinamide riboside (NR), an precursor, stimulates peroxisome proliferator-activated receptor alpha signaling restores FAO proximal tubules, thereby protecting CKD sexes. Bulk RNA-sequencing shows pathways mice activated by NR These transcriptional changes confirmed orthogonal imaging techniques biochemical assays. Single nuclei spatial transcriptomics, first their kind mice, tubule cells. Finally, also report, for time, sex differences at level this model. In summary, identify nephroprotective demonstrate cells substantially contribute to benefit.

Язык: Английский

Процитировано

1