Bioactive Materials, Год журнала: 2023, Номер 25, С. 160 - 175
Опубликована: Фев. 2, 2023
3D bioprinting holds great promise toward fabricating biomimetic living constructs in a bottom-up assembly manner. To date, various emergences of have been bioprinted for
Язык: Английский
Frontiers in Neurology, Год журнала: 2019, Номер 10
Опубликована: Март 22, 2019
Traumatic spinal cord injury (SCI) is a life changing neurological condition with substantial socioeconomic implications for patients and their care-givers. Recent advances in medical management of SCI has significantly improved diagnosis, stabilization, survival rate well-being patients. However, there been small progress on treatment options improving the outcomes This incremental success mainly reflects complexity pathophysiology diverse biochemical physiological changes that occur injured cord. Therefore, past few decades, considerable efforts have made by researchers to elucidate unravel underlying cellular molecular mechanisms tissue degeneration repair To this end, number preclinical animal models developed more closely recapitulate primary secondary processes SCI. In review, we will provide comprehensive overview recent our understanding We also discuss human available experimental model systems employed identify develop therapeutic strategies condition.
Язык: Английский
Процитировано
1084
International Journal of Molecular Sciences, Год журнала: 2020, Номер 21(20), С. 7533 - 7533
Опубликована: Окт. 13, 2020
Spinal cord injury (SCI) is a destructive neurological and pathological state that causes major motor, sensory autonomic dysfunctions. Its pathophysiology comprises acute chronic phases incorporates cascade of events such as ischemia, oxidative stress, inflammatory events, apoptotic pathways locomotor Many therapeutic strategies have been proposed to overcome neurodegenerative reduce secondary neuronal damage. Efforts also devoted in developing neuroprotective neuro-regenerative therapies promote recovery outcome. Although varying degrees success achieved, curative accomplishment still elusive probably due the complex healing protective mechanisms involved. Thus, current understanding this area must be assessed formulate appropriate treatment modalities improve SCI recovery. This review aims pathophysiology, interrelated or interlinked multimolecular interactions various methods i.e., neuroprotective, immunomodulatory relevant approaches.
Язык: Английский
Процитировано
866
Nature Communications, Год журнала: 2019, Номер 10(1)
Опубликована: Авг. 28, 2019
Abstract Traumatic spinal cord injury results in severe and irreversible loss of function. The triggers a complex cascade inflammatory pathological processes, culminating formation scar. While traditionally referred to as glial scar, the scar fact comprises multiple cellular extracellular components. This multidimensional nature should be considered when aiming understand role scarring limiting tissue repair recovery. In this Review we discuss recent advances understanding composition phenotypic characteristics oversimplification defining binary terms good or bad, development therapeutic approaches target components enable improved functional outcome after injury.
Язык: Английский
Процитировано
556
Nano Letters, Год журнала: 2020, Номер 20(6), С. 4298 - 4305
Опубликована: Май 7, 2020
Spinal cord injury is among the most fatal diseases. The complicated inhibitory microenvironment requires comprehensive mitigation. Exosomes derived from mesenchymal stem cells (MSCs) are natural biocarriers of cell paracrine secretions that bear functions regulation. However, effective retention, release, and integration exosomes into injured spinal tissue poorly defined. Herein, an innovative implantation strategy established using human MSC-derived immobilized in a peptide-modified adhesive hydrogel (Exo-pGel). Unlike systemic admistration exosomes, topical transplantation Exo-pGel provides exosome-encapsulated extracellular matrix to nerve tissue, thereby inducing effecient mitigation SCI microenvironment. implanted exhibit efficient retention sustained release host tissues. elicits significant recovery urinary preservation by effectively mitigating inflammation oxidation. therapy presents promising for treatment central nervous system diseases based on exosome implantation.
Язык: Английский
Процитировано
324
Nature, Год журнала: 2020, Номер 587(7835), С. 613 - 618
Опубликована: Окт. 7, 2020
Язык: Английский
Процитировано
317
International Journal of Molecular Sciences, Год журнала: 2019, Номер 20(11), С. 2698 - 2698
Опубликована: Май 31, 2019
Spinal cord injury (SCI) constitutes an inestimable public health issue. The most crucial phase in the pathophysiological process of SCI concerns well-known secondary injury, which is uncontrolled and destructive cascade occurring later with aberrant molecular signaling, inflammation, vascular changes, cellular dysfunctions. use mesenchymal stem cells (MSCs) represents one important promising tested strategies. Their appeal, among other sources types cells, increased because their ease isolation/preservation properties. Nevertheless, encouraging promise from preclinical studies was followed by weak conflicting results clinical trials. In this review, therapeutic role MSCs discussed, together properties, application, limitations, future perspectives.
Язык: Английский
Процитировано
303
Science, Год журнала: 2021, Номер 374(6569), С. 848 - 856
Опубликована: Ноя. 11, 2021
Fibril motion improves peptide signaling Artificial scaffolds that bear the peptide-signaling sequences of proteins for tissue regeneration often have limited effectiveness. Álvarez et al . synthesized supramolecular fibril bearing two promote nerve regeneration, one reduces glial scarring and another promotes blood vessel formation (see Perspective by Wojciechowski Stevens). In a mouse model paralyzing human spinal cord injury, mutations in tetrapeptide domain outside regions improved recovery promoting intense within fibrils. The mutation with most dynamics resulted corticospinal axon regrowth myelination, functional revascularization, motor neuron survival. —PDS
Язык: Английский
Процитировано
274
Nature Communications, Год журнала: 2018, Номер 9(1)
Опубликована: Ноя. 1, 2018
Abstract Spinal cord injury leads to a massive response of innate immune cells in non-regenerating mammals, but also successfully regenerating zebrafish. However, the role successful regeneration is poorly defined. Here we show that inhibiting inflammation reduces and promoting it accelerates axonal spinal-lesioned zebrafish larvae. Mutant analyses peripheral macrophages, not neutrophils or microglia, are necessary for repair. Macrophage-less irf8 mutants prolonged with elevated levels Tnf-α Il-1β. Inhibiting does rescue growth mutants, impairs wildtype animals, indicating pro-regenerative Tnf-α. In contrast, decreasing Il-1β number + functional mutants. during early regeneration, interference function animals. Hence, dynamically controlled by macrophages promote spinal
Язык: Английский
Процитировано
245
Nature reviews. Neuroscience, Год журнала: 2020, Номер 21(7), С. 366 - 383
Опубликована: Июнь 9, 2020
Язык: Английский
Процитировано
222
Nature Communications, Год журнала: 2020, Номер 11(1)
Опубликована: Сен. 9, 2020
The strategies concerning modification of the complex immune pathological inflammatory environment during acute spinal cord injury remain oversimplified and superficial. Inspired by acidic microenvironment at sites, a functional pH-responsive immunoregulation-assisted neural regeneration strategy was constructed. With capability directly responding to focal areas followed triggered release IL-4 plasmid-loaded liposomes within few hours suppress cytokines promote differentiation mesenchymal stem cells in vitro, microenvironment-responsive immunoregulatory electrospun fibers were implanted into rats. Together with sustained nerve growth factor (NGF) achieved microsol core-shell structure, immunological fiber scaffolds revealed bring significantly shifted subtype down-regulate inflammation response, reduce scar tissue formation, angiogenesis as well site, enhance recovery vivo. Overall, this provided delivery system through regulation effect so break current dilemma from contradiction between response regeneration, providing an alternative for treatment injury.
Язык: Английский
Процитировано
178