Transcriptomic adaptation of skeletal muscle in response to MICT and HIIT exercise modalities

PLoS ONE, Год журнала: 2025, Номер 20(2), С. e0318782 - e0318782

Опубликована: Фев. 25, 2025

Skeletal muscle exhibits remarkable plasticity in response to diverse stimuli, with exercise serving as a potent trigger. Varied modalities, including moderate-intensity continuous training (MICT) and high-intensity interval (HIIT), induce distinct structural functional adaptations on skeletal muscle. However, the underlying molecular mechanisms governing these remain poorly understood. In this study, we utilized RNA-seq characterize transcriptomic profile of murine gastrocnemius following 8-week treadmill-based MICT (M group) HIIT (H group). A total 1052 DEGs were screened H vs. M. Among top 10 significant DEGs, Foxo1 Myod1 are closely related muscular physiology. Through KEGG pathway analysis, primarily identified FoxO, MAPK, PI3K-AKT pathways. By analyzing expression myokines, significantly higher Igf-1 level was observed M group compared group. Therefore, IGF-1, well-known upstream regulator both PI3K-AKT-FoxO MAPK pathways, might drive through variations induced by two modalities.

Язык: Английский

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Sprinters’ and Marathon Runners’ Performances Are Better Explained by Muscle Fibers’ Percentage Cross-Sectional Area than Any Other Parameter of Muscle Fiber Composition

Sports, Год журнала: 2025, Номер 13(3), С. 74 - 74

Опубликована: Март 4, 2025

The present study aimed to investigate the correlation between muscle fiber type variables and sprinting, jumping, strength, power endurance performances in sprinters marathon runners. Furthermore, explored which variable influences athletes’ performance most discriminates from Body composition, vastus lateralis maximum isometric force, rate of force development were assessed well-trained (n = 15; age: 24.7 ± 4.1 years; body mass: 82.7 8.8 kg; fat: 11.3 4.2%) runners 26.1 3.2 69.7 4.3 9.5 3.3%). Significant correlations observed composition (r −0.848 0.902; p < 0.05), with percentage cross-sectional areas (%CSAs) showing strongest associations. Regression analyses confirmed %CSAs as predictors (R2: 0.796–0.978; 0.001; B coefficients: −0.293–0.985), while discriminant analysis accurately differentiated (100%, 0.001) by only using fibers’ %CSAs. In conclusion, may be critical for explaining distinguishing sprinters’ runners’ performances.

Язык: Английский

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Comparable amino acid & intramuscular signalling responses following consumption of microflora compared to whey protein post-resistance exercise in young adults

Опубликована: Март 1, 2025

Язык: Английский

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Skeletal muscle atrophy and dysfunction in obesity and type-2 diabetes mellitus: Myocellular mechanisms involved

Reviews in Endocrine and Metabolic Disorders, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Abstract Obesity and type-2 diabetes mellitus (T2DM) are interrelated metabolic disorders primarily driven by overnutrition physical inactivity, which oftentimes entails a transition from obesity to T2DM. Compromised musculoskeletal health consistently emerges as common hallmark in the progression of these disorders. Skeletal muscle atrophy dysfunction can further impair whole-body metabolism reduce exercise capacity, thus instigating vicious cycle that deteriorates underlying conditions. However, myocellular repercussions disturbances remain be completely clarified. Insulin signaling not only facilitates skeletal glucose uptake but also plays central role anabolism mainly due suppression catabolic pathways facilitating an anabolic response nutrient feeding. Chronic may trigger different mechanisms proposed contribute insulin resistance aggravate dysfunction. These include inactivation components through sustained activation stress-related pathways, mitochondrial dysfunction, shift glycolytic fibers, hyperglycemia. In present review, we aim delve on mechanisms, providing overview processes involved under chronic overnutrition, their contribution

Язык: Английский

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Rapamycin does not compromise physical performance or muscle hypertrophy after PoWeR while intermittent rapamycin alleviates glucose disruptions by frequent rapamycin

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

An increasing number of physically active adults are taking the mTOR inhibitor rapamycin off label with goal extending healthspan. However, frequent dosing disrupts metabolic health during sedentary conditions and abates anabolic response to exercise. Intermittent once weekly minimizes many negative side effects in mice. it remains unknown how different schedules impact metabolic, physical, skeletal muscle adaptations voluntary exercise training. Therefore, we tested hypothesis that intermittent (2mg/kg; 1x/week) would avoid detrimental on 8 weeks progressive weighted wheel running (PoWeR) adult female mice (5-month-old) by evading sustained inhibitory signaling more 3x/week). Frequent but not suppressed mTORC1 PoWeR trained improved maximal capacity, absolute grip strength, myofiber hypertrophy no differences between vehicle or treated Conversely, had impaired glucose tolerance insulin sensitivity compared after PoWeR; however, reduced intolerance versus rapamycin. Collectively, these data suggest 1) is largely compatible physical benefits 2) body composition metabolism context may be dosing.

Язык: Английский

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