Experimental Eye Research, Год журнала: 2020, Номер 199, С. 108185 - 108185
Опубликована: Авг. 22, 2020
Язык: Английский
Pathology & Oncology Research, Год журнала: 2019, Номер 25(3), С. 859 - 874
Опубликована: Фев. 21, 2019
Язык: Английский
Процитировано
177
Biomedicine & Pharmacotherapy, Год журнала: 2019, Номер 118, С. 109129 - 109129
Опубликована: Июль 19, 2019
Maternally expressed gene 3 (MEG3) is a long non-coding RNA (lncRNA) located on chromosome 14q32.3. Direct sequencing experiments have shown monoallelic expression of this lncRNA. Several studies down-regulation lncRNA in human cancers. In some cases, hypermethylation the promoter region has been suggested as underlying mechanism. Functional that controls several tumor suppressor genes and oncogenes among them are p53, RB, MYC TGF-β. Through regulation Wnt-β-catenin pathway, it also affects epithelial-mesenchymal transition. vitro demonstrated contribution MEG3 defining response to chemotherapeutic agents such paclitaxel, cisplatin oxaliplatin. Certain polymorphisms within implicated cancer risk (rs7158663, rs4081134 rs11160608) or therapeutic patients (rs10132552). Taken together, regarded putative biomarker treatment target. current review, aspects participation carcinogenesis discussed.
Язык: Английский
Процитировано
153
International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(16), С. 9353 - 9353
Опубликована: Авг. 19, 2022
Although the first discovery of a non-coding RNA (ncRNA) dates back to 1958, only in recent years has complexity transcriptome started be elucidated. However, its components are still under investigation and their identification is one challenges that scientists presently facing. In addition, function far from being fully understood. The portion genome indeed largest, both quantitatively qualitatively. A large fraction these ncRNAs have regulatory role either coding mRNAs or other ncRNAs, creating an intracellular network crossed interactions (competing endogenous networks, ceRNET) fine-tune gene expression health disease. alteration equilibrium among such can enough cause transition disease, but opposite equally true, leading possibility intervening based on mechanisms cure human conditions. this review, we summarize present knowledge mechanisms, illustrating how they used for disease treatment, current pitfalls, roles environmental lifestyle-related contributing factors, addition ethical, legal, social issues arising (improper) use.
Язык: Английский
Процитировано
61
Frontiers in Cell and Developmental Biology, Год журнала: 2022, Номер 10
Опубликована: Дек. 5, 2022
Long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) is a lncRNA located at the DLK1-MEG3 site of human chromosome 14q32.3. The expression MEG3 in various tumors substantially lower than that normal adjacent tissues, and deletion involved occurrence many tumors. high could inhibit development through several mechanisms, which has become research hotspot recent years. As member tumor suppressor lncRNAs, expected to be new target for diagnosis treatment. This review discusses molecular mechanisms different future challenges treatment cancers MEG3.
Язык: Английский
Процитировано
42
Cell Death and Disease, Год журнала: 2025, Номер 16(1)
Опубликована: Фев. 24, 2025
Abstract While apoptotic cell death is known to be central the pathogenesis of radiation-induced liver injury (RILI), mechanistic basis for this activity remains poorly understood. N 6 -methyladenosine (m A) modifications are most common form reversible methylation observed on lncRNAs in eukaryotic cells, with their presence leading pronounced changes a range biological processes. The degree which m A modification plays role induction response ionizing radiation (IR) context RILI established. Here, IR-induced apoptosis was found significantly decrease levels present, expression methyltransferase-like 3 (METTL3) at 2 d post vitro. From perspective, methylated RNA immunoprecipitation assay that lncRNA MEG3 major METTL3 target. upregulated via METTL3-mediated process dependent YTHDC1, ultimately reversing miR-20b-mediated inhibition BNIP2 expression. Together, these findings demonstrate responsivity IR death, reverse through YTHDC1-dependent MEG3, suggesting may play incidence.
Язык: Английский
Процитировано
1
Cancer Cell International, Год журнала: 2020, Номер 20(1)
Опубликована: Янв. 10, 2020
Melanoma is the most aggressive type of skin cancer with high mortality rate and poor prognosis. lncRNA MEG3, a tumor suppressor, closely related to development various cancers. However, role MEG3 in melanoma has seldom been studied.RT-PCR was used examine expressions E-cadherin patients cell lines. Then, biological functions were demonstrated by transfecting MEG3-siRNA, MEG3-overexpression, E-cadherin-siRNA E-cadherin-overexpression plasmids Moreover, CCK8 assay colony formation utilized assess proliferation; Transwell performed evaluate invasive ability; xenografts nude mice applied test generation. Additionally, target interactions among miR-21 determined dual luciferase reporter assay. Finally, RT-PCR WB further conducted verify regulatory roles E-cadherin.The clinical data showed that both declined carcinoma tissues as compared their para-carcinoma tissues. B16 cells also higher than those A375 A2058 cells. Subsequently, based on differently expressed these human lines, we chose B16, for following experiments. The results could suppress growth, metastasis formation; meanwhile had same effects formation. Furthermore, analysis Kaplan-Meier curves confirmed there positive correlation between E-cadherin. Ultimately, assays directly which turn. revealed knockdown inhibited expression promoted expression, but overexpression presented completely opposite results.Our findings indicated might inhibit modulating miR-21/E-cadherin axis.
Язык: Английский
Процитировано
55
Neuro-Oncology, Год журнала: 2020, Номер 22(12), С. 1771 - 1784
Опубликована: Май 21, 2020
Glioblastoma (GBM) stemlike cells (GSCs) are thought to be responsible for the maintenance and aggressiveness of GBM, most common primary brain tumor in adults. This study aims at elucidating involvement deregulations within imprinted delta-like homolog 1 gene‒type III iodothyronine deiodinase gene (DLK-DIO3) region on chromosome 14q32 GBM pathogenesis.Real-time PCR analyses were performed GSCs tissues. Methylation analyses, expression, reverse-phase protein array profiles used investigate suppressor function maternally expressed 3 (MEG3).Loss expression genes noncoding RNAs DLK1-DIO3 was observed tissues compared with normal brain. downregulation is mainly mediated by epigenetic silencing. Kaplan-Meier analysis indicated that low MEG3 MEG8 long (lnc)RNAs significantly correlated short survival patients. restoration impairs tumorigenic abilities vitro inhibiting cell growth, migration, colony formation decreases vivo reducing infiltrative growth. These effects associated modulation involved adhesion epithelial-to-mesenchymal transition (EMT).In acts as a regulating adhesion, EMT, proliferation, thus providing potential candidate novel therapies.
Язык: Английский
Процитировано
51
Biomolecules, Год журнала: 2021, Номер 11(11), С. 1602 - 1602
Опубликована: Окт. 29, 2021
Long noncoding RNAs (lncRNAs) are transcripts greater than 200 nucleotides that do not code for proteins but regulate gene expression. Recent studies indicate lncRNAs involved in the modulation of biological functions human disease. KCNQ1 Opposite Strand/Antisense Transcript 1 (KCNQ1OT1) encodes a lncRNA from opposite strand CDKN1C/KCNQ1OT1 cluster is reported to play vital role development and progression cancer. KCNQ1OT1 regulates cancer cell proliferation, cycle, migration invasion, metastasis, glucose metabolism, immune evasion. The aberrant expression patients associated with poor prognosis decreased survival. This review summarizes recent literature related molecular mechanisms various cancers, including colorectal, bladder, breast, oral, melanoma, osteosarcoma, lung, glioma, ovarian, liver, acute myeloid leukemia, prostate, gastric. We also discuss as promising diagnostic biomarker novel therapeutic target cancers.
Язык: Английский
Процитировано
49
Genomics, Год журнала: 2021, Номер 113(4), С. 1689 - 1704
Опубликована: Апрель 9, 2021
Язык: Английский
Процитировано
43
Cells, Год журнала: 2022, Номер 11(3), С. 577 - 577
Опубликована: Фев. 7, 2022
Long non-coding RNAs (lncRNAs) are key regulators of numerous intracellular processes leading to tumorigenesis. They frequently deregulated in cancer, functioning as oncogenes or tumor suppressors. As they act through multiple mechanisms, it is not surprising that may exert dual functions the same tumor. In melanoma, a highly invasive and metastatic with propensity rapidly develop drug resistance, lncRNAs play different roles in: (i) guiding phenotype switch metastasis formation; (ii) predicting response melanoma patients immunotherapy; (iii) triggering adaptive responses therapy acquisition resistance phenotypes. this review we summarize most recent findings on involved growth spreading distant sites, focusing their role biomarkers for disease diagnosis patient prognosis, targets novel therapeutic approaches.
Язык: Английский
Процитировано
33