Effects of Clostridium butyricum on Intestinal Microflora and Metabolism of Eriocheir sinensis

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(18), С. 13784 - 13784

Опубликована: Сен. 7, 2023

Clostridium butyricum, a new probiotic in recent years, can produce butyric acid and short-chain fatty acids. It has the characteristics of strong alkali resistance, high temperature resistance to most antibiotics, more advantages than other probiotics. However, action mechanism C. butyricum on Eriocheir sinensis is still unclear needs further study. In this study, when was added basic diet, number living bacteria 0, 1 × 106 108 CFU/g, respectively. The E. were randomly divided into three groups: (blank control group, experimental group (1 CFU/g) 2 CFU/g)). They fed an diet for 28 days. effects studied by detecting differences non-specific immune indexes, intestinal microflora, metabolites between serum hepatopancreas. results showed that could improve antioxidant ability hepatopancreas, protect tissues, promote absorption nutrients. At same time, it enhance microbial diversity richness gut flora. LC-MS metabolomics used detect metabolism found up-regulate lysophosphatidylcholine intestine. Through KEGG enrichment pathway, significantly different mainly concentrated six metabolic pathways. purine alanine, aspartate, glutamate pathways downward trend, indicating addition feed reduce metabolism, water-salt balance organism's cells, inflammation. certain concentrations sinensis, flora environment, growth beneficial gut. This body's which conducive its growth.

Язык: Английский

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The role of pattern recognition receptors in the innate immune system of Chinese mitten crab (Eriocheir sinensis)

Fish & Shellfish Immunology, Год журнала: 2024, Номер 154, С. 109946 - 109946

Опубликована: Окт. 5, 2024

Язык: Английский

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Transcriptome analysis of eyestalk ganglion provides new insights into the immune response of Eriocheir sinensis

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Год журнала: 2025, Номер unknown, С. 101492 - 101492

Опубликована: Март 1, 2025

Язык: Английский

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Carapace Morphological Characteristics of Chinese Mitten Crab (Eriocheir sinensis) from Emerging Origins Revealed via Geometric Morphometrics

Animals, Год журнала: 2025, Номер 15(9), С. 1300 - 1300

Опубликована: Апрель 30, 2025

This study used geometric morphometrics to investigate the carapace features of Chinese mitten crabs (Eriocheir sinensis) from non-traditional/emerging origins (Zhenlai County and Bosten Lake) identify differences in relative traditional (Yangcheng Lake). Thirty-five landmark data points on crab were extracted based point approach standardized obtain mean shape three origins. The morphologies different varied. Compared average morphology, first lateral tooth Zhenlai was shorter, M pattern enlarged males, whereas second teeth other two longer, reduced. In female crabs, distinct morphological variations observed across regions. enlarged. frontal posterior margin narrowed. Yangcheng Lake third elongated, discrimination accuracy individual male reached 100%. results confirm that morphometric method represents a convenient non-lethal tracing E. sinensis fine-scale production areas. Thus, this provides guidance developing characteristic industrial chain for aquatic products emerging origins, which may help promote sustainable development aquaculture industry associated

Язык: Английский

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Laboratory evaluation of ELISA and indirect immunofluorescence assay in response to emergence of Japanese encephalitis virus genotype IV in Australia

Journal of Clinical Virology, Год журнала: 2023, Номер 168, С. 105580 - 105580

Опубликована: Сен. 9, 2023

The unexpected recent emergence of Japanese encephalitis virus (JEV) genotype IV in multiple southern states Australia necessitated an evaluation JEV serological tests suitable for diagnosing acute infection and seroprevalence studies. This study examined the analytical clinical performance two high-throughput assays, Euroimmun immunofluorescence assay (IFA) enzyme-linked immunosorbent (ELISA), across four cohorts; (1) surveillance piggery workers outbreak areas, (2) residents (3) (4) post-JEV vaccination. ELISA IFA IgM demonstrated minimal cross-reactivity (0-1.8%) with other endemic flaviviruses, high sensitivity (100%) this low endemicity setting. Differences IgG serodynamics between assays suggest convalescent paired testing are critical infection. High concordance was observed when used serosurveillance (97.4% agreement, Cohen' κ 0.74 [95% CI 0.614-0.860]) vaccination cohorts (91.1% Cohen's 0.806 0.672-0.941]). In conclusion, highlights & epidemiological applications limitations these commercial assays.

Язык: Английский

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Bacterial recognition and inhibition activities of an LRR-only protein in the Chinese mitten crab, Eriocheir sinensis

Fish & Shellfish Immunology, Год журнала: 2024, Номер 152, С. 109799 - 109799

Опубликована: Авг. 3, 2024

Язык: Английский

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Morphological transformation and pathogenicity of Metschnikowia bicuspidata in Chinese mitten crab (Eriocheir sinensis)

Aquaculture, Год журнала: 2024, Номер unknown, С. 741921 - 741921

Опубликована: Ноя. 1, 2024

Язык: Английский

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Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensis

Aquaculture Reports, Год журнала: 2024, Номер 40, С. 102607 - 102607

Опубликована: Дек. 31, 2024

Язык: Английский

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Bacterial Recognition and Inhibition Activities of an Lrr-Only Protein in the Chinese Mitten Crab, Eriocheir Sinensis

Опубликована: Янв. 1, 2024

Язык: Английский

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