International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(8), С. 4196 - 4196
Опубликована: Апрель 18, 2021
Thyroid
cancers
are
the
most
common
of
endocrine
system
malignancies
and
progress
must
be
made
in
areas
differential
diagnosis
treatment
to
improve
patient
management.
Advances
understanding
carcinogenic
mechanisms
have
occurred
various
fronts,
including
studies
chaperone
(CS).
Components
CS
found
quantitatively
increased
or
decreased,
some
correlations
been
established
between
quantitative
changes
tumor
type,
prognosis,
response
treatment.
These
provide
basis
for
identifying
distinctive
patterns
useful
planning
experiments
aiming
at
elucidating
role
tumorigenesis.
Here,
we
discuss
components
thyroid
(TC).
The
chaperones
belonging
families
small
heat-shock
proteins
Hsp70
Hsp90
chaperonin
Group
I,
Hsp60,
quantified
mostly
by
immunohistochemistry
Western
blot
normal
control
tissues
extracellular
vesicles.
Distinctive
differences
were
revealed
types.
frequent
finding
was
an
increase
chaperones,
which
can
attributed
augmented
need
cells
because
their
accelerated
metabolism,
growth,
division
rate.
Thus,
help
cell
rather
than
protect
patient,
exemplifying
chaperonopathies
mistake
collaborationism.
This
highlights
research
on
chaperonotherapy,
namely
development
means
eliminate/inhibit
pathogenic
chaperones.
Biomarker Research,
Год журнала:
2022,
Номер
10(1)
Опубликована: Янв. 9, 2022
Abstract
Zinc
finger
proteins
are
transcription
factors
with
the
domain,
which
plays
a
significant
role
in
gene
regulation.
As
largest
family
of
human
genome,
zinc
(ZNF)
characterized
by
their
different
DNA
binding
motifs,
such
as
C2H2
and
Gag
knuckle.
Different
kinds
motifs
exhibit
wide
variety
biological
functions.
have
been
reported
various
diseases,
especially
several
cancers.
Hepatocellular
carcinoma
(HCC)
is
third
leading
cause
cancer-associated
death
worldwide,
China.
Most
HCC
patients
suffered
from
hepatitis
B
virus
(HBV)
C
(HCV)
injection
for
long
time.
Although
surgical
operation
has
extremely
developed,
prognosis
still
very
poor,
underlying
mechanisms
tumorigenesis
not
completely
understood.
Here,
we
summarize
multiple
functions
recent
research
progression.
We
also
discuss
significance
diagnosis
prognostic
evaluation.
The
exponential
increase
in
our
ability
to
harness
multi-dimensional
biological
and
clinical
data
from
experimental
real-world
settings
has
transformed
pharmaceutical
research
development
recent
years,
with
increasing
applications
of
artificial
intelligence
(AI)
machine
learning
(ML).
Patient-centered
iterative
forward
reverse
translation
is
at
the
heart
precision
medicine
discovery
across
continuum
target
validation
optimization
pharmacotherapy.
Integration
advanced
analytics
into
practice
Translational
Medicine
now
a
fundamental
enabler
fully
exploit
information
contained
diverse
sources
big
sets
such
as
"omics"
data,
illustrated
by
deep
characterizations
genome,
transcriptome,
proteome,
metabolome,
microbiome,
exposome.
In
this
commentary,
we
provide
an
overview
ML
drug
development,
aligned
three
strategic
pillars
(target,
patient,
dose)
offer
perspectives
on
their
potential
transform
science
discipline.
Opportunities
for
integrating
approaches
discipline
Pharmacometrics
are
discussed
will
revolutionize
model-informed
development.
Finally,
posit
that
joint
efforts
Clinical
Pharmacology,
Bioinformatics,
Biomarker
Technology
experts
vital
cross-functional
team
realize
promise
AI/ML-enabled
Precision
Medicine.
Determination
of
malignancy
in
thyroid
nodules
remains
a
major
diagnostic
challenge.
Here
we
report
the
feasibility
and
clinical
utility
developing
an
AI-defined
protein-based
biomarker
panel
for
classification
nodules:
based
initially
on
formalin-fixed
paraffin-embedded
(FFPE),
further
refined
fine-needle
aspiration
(FNA)
tissue
specimens
minute
amounts
which
pose
technical
challenges
other
methods.
We
first
developed
neural
network
model
19
protein
biomarkers
proteomes
1724
FFPE
samples
from
retrospective
cohort.
This
classifier
achieved
over
91%
accuracy
discovery
set
classifying
malignant
nodules.
The
was
externally
validated
by
blinded
analyses
cohort
288
(89%
accuracy;
FFPE)
prospective
294
FNA
biopsies
(85%
accuracy)
twelve
independent
centers.
study
shows
that
integrating
high-throughput
proteomics
AI
technology
multi-center
cohorts
facilitates
precise
disease
diagnosis
is
otherwise
difficult
to
achieve
Experimental & Molecular Medicine,
Год журнала:
2023,
Номер
55(8), С. 1757 - 1769
Опубликована: Авг. 1, 2023
Solute
carrier
family
39
member
10
(SLC39A10)
belongs
to
a
subfamily
of
zinc
transporters
and
plays
key
role
in
B-cell
development.
Previous
studies
have
reported
that
its
upregulation
promotes
breast
cancer
metastasis
by
enhancing
the
influx
ions
(Zn
Clinical and Translational Medicine,
Год журнала:
2022,
Номер
12(6)
Опубликована: Июнь 1, 2022
Abstract
Background
Studies
on
biological
functions
of
N6‐methyladenosine
(m
6
A)
modification
in
mRNA
have
sprung
up
recent
years.
Previous
studies
reported
m
A
can
determine
fate
and
play
a
pivotal
role
tumour
development
progression.
The
zinc
finger
protein
677
(ZNF677)
belongs
to
the
family
possesses
transcription
factor
activity
by
binding
sequence‐specific
DNA.
Methods
expression
ZNF677
its
clinicopathological
impact
were
evaluated
renal
cell
carcinoma
(RCC)
patients.
level
was
determined
methylated
RNA
immunoprecipitation‐sequencing
(MeRIP‐seq)
MeRIP‐qPCR
RCC
tissues
adjacent
normal
tissues.
immunoprecipitation‐qPCR
(RIP‐qPCR)
luciferase
assays
performed
identify
targeted
effect
IGF2BP2
YTHDF1
ZNF677.
cells
subcutaneous
models
uncovered
CRISPR/dCas13b‐METTL3
growth.
ZNF677‐binding
sites
CDKN3
promoter
investigated
chromatin
immunoprecipitation
(ChIP)
assays.
Results
is
frequently
downregulated
low
associated
with
unfavourable
prognosis
decreased
level.
Further,
we
find
A‐modified
coding
sequence
(CDS)
positively
regulates
translation
stability
via
IGF2BP2,
respectively.
Targeted
specific
methylation
CRISPR/dCas13b‐METLL3
system
significantly
increase
ZNF677,
dramatically
inhibit
proliferation
induce
apoptosis
cells.
In
addition,
exerted
suppressor
through
transcriptional
repression
promoter.
vitro
clinical
data
confirm
negative
roles
ZNF677/CDKN3
growth
progression
RCC.
Conclusion
as
silenced
RCC,
which
may
highlight
methylation‐based
approach
for
diagnosis
therapy.
Heliyon,
Год журнала:
2024,
Номер
unknown, С. e26061 - e26061
Опубликована: Фев. 1, 2024
BackgroundAlthough
many
experiments
and
clinical
studies
have
proved
the
link
between
expression
of
CDKN3
human
tumors,
we
not
been
able
to
identify
any
bioinformatics
study
in
which
extensive
tumor-promoting
effect
was
systematically
analyzed.ObjectiveExplore
effects
review
research
progress
cancer.MethodsWe
reviewed
literature
on
tumors.
We
explored
potential
different
tumors
TCGA
database
GTEx
using
multiple
platforms
websites.
studied
level
CDKN3,
survival,
prognosis,
diagnosis,
genetic
variation,
immune
infiltration,
enrichment
analysis
databases
such
as
TIMER
2.0,
GEPIA2,
cBioPortal,
STRING.ResultsWe
found
that
is
highly
expressed
most
The
closely
related
prognosis
some
And
may
diagnostic
value.
conclusion
our
roughly
same,
but
there
are
differences,
worthy
further
study.
Moreover,
be
cell
infiltration
tumor
tissues.
alteration
LUAD,
STAD,
SARC,
PCPG,
ESCA
with
“Amplification”
main
type.
In
addition,
through
analysis,
affects
mainly
control
cycle
mitosis.ConclusionCDKN3
tissues
has
a
statistical
correlation
survival
prognosis.
It
mechanisms
infiltration.
Journal of Cellular Physiology,
Год журнала:
2018,
Номер
234(8), С. 12809 - 12820
Опубликована: Дек. 7, 2018
Abstract
Long
noncoding
RNAs
have
been
reported
to
be
important
regulators
in
numerous
cancers.
In
this
study,
we
found
that
HOXC13
antisense
RNA
(HOXC13‐AS)
was
highly
expressed
head
and
neck
squamous
carcinoma
(HNSC)
tissues
The
Cancer
Genome
Atlas
database.
Nasopharyngeal
(NPC)
belongs
HNSC.
Therefore,
further
investigated
the
potential
role
of
HOXC13‐AS
NPC.
Quantitative
reverse
transcription
polymerase
chain
reaction
examination
revealed
markedly
upregulated
NPC
cell
lines.
Furthermore,
identified
as
an
independent
prognosis
factor
by
Cox
regression
analyses.
Subsequently,
functional
assay
knockdown
impaired
proliferation,
migration,
invasion.
Mechanistically,
RIP
luciferase
reporter
analysis
confirmed
miR‐383‐3p
a
target
HOXC13‐AS.
Besides,
high
mobility
group
AT‐hook
2
(HMGA2)
proved
Finally,
rescue
assays
demonstrated
functioned
competing
endogenous
enhance
expression
HMGA2
via
sponging
miR‐383‐3p.
This
study
suggested
exerted
oncogenic
function
regulating
miR‐383‐3p/HMGA2
axis,
indicating
may
therapeutic
for
patients
with
Cell Death and Differentiation,
Год журнала:
2021,
Номер
28(8), С. 2450 - 2464
Опубликована: Март 19, 2021
Small
nucleolar
RNA
SNORD50A
and
SNORD50B
(SNORD50A/B)
has
been
reported
to
be
recurrently
deleted
function
as
a
putative
tumor
suppressor
in
different
types
of
cancer
by
binding
suppressing
the
activity
KRAS
oncoproteins.
Its
deletion
correlates
with
poorer
patient
survival.
However,
this
study,
we
surprisingly
found
that
SNORD50A/B
loss
predicted
better
survival
breast
patients
carrying
wild-type
p53.
Functional
studies
showed
strongly
inhibited
proliferation,
migration,
invasion
tumorigenic
potential,
induced
cell
cycle
arrest
apoptosis
p53
cells,
while
exerted
opposite
effects
mutated
cells.
This
was
also
supported
ectopically
expressing
both
Mechanistically,
clearly
enhances
interaction
between
E3
ubiquitin
ligase
TRIM21
its
substrate
GMPS
forming
complex
among
them,
thereby
promoting
ubiquitination
subsequent
cytoplasmic
sequestration.
cells
will
release
induce
translocation
into
nucleus,
where
can
recruit
USP7
form
p53,
decreasing
ubiquitination,
stabilizing
proteins,
inhibiting
malignant
phenotypes
Altogether,
present
study
first
reports
plays
an
oncogenic
role
cancers
mediating
TRIM21-GMPS
interaction.
