Frontiers in Immunology,
Год журнала:
2022,
Номер
13
Опубликована: Фев. 15, 2022
Exosomes
are
nanoscale
extracellular
vesicles
(EVs),
which
present
in
all
body
fluids
tested.
They
secreted
by
a
variety
of
cells
including
macrophages,
dendritic
cells,
mast
granulocytes,
lymphocytes,
and
tumor
cells.
different
have
biological
components
functional
characteristics
play
an
important
role
many
pathophysiological
activities.
Recent
studies
revealed
that
exosomes
can
regulate
the
occurrence
development
inflammatory
immune
diseases
tumors
transmitting
their
unique
proteins,
lipids,
nucleic
acids
as
signaling
molecules
to
other
serve
novel
class
diagnostic
biomarkers
drug
delivery
systems
with
promising
applications
immunotherapy,
particularly
because
breakthroughs
nanotechnology
led
exploration
engineered
for
immunotargeted
therapies.
Therefore,
here
we
review
progress
being
made
on
application
immunotherapy
its
multiple
regulatory
mechanisms
explore
potential
future.
Therapeutic Advances in Chronic Disease,
Год журнала:
2021,
Номер
12
Опубликована: Янв. 1, 2021
Evidence
has
demonstrated
that
non-coding
RNAs
(ncRNAs)
could
be
delivered
efficiently
to
recipient
cells
using
exosomes
as
a
carrier.
Additionally,
long
ncRNA
nuclear
enriched
abundant
transcript
1
(NEAT1)
is
emerging
vital
regulatory
molecule
in
the
progression
of
rheumatoid
arthritis
(RA).
The
aim
this
study
was
identify
NEAT1/miR-144-3p/Rho-associated
protein
kinase
2
(ROCK2)
functional
network
regulating
WNT
signaling
pathway
RA.In
vivo,
collagen-induced
(CIA)
model
established
analyze
effects
blood
on
incidence,
clinical
score,
and
bone
degradation
RA.
In
vitro,
CD4+T
were
characterized
by
flow
cytometry
cell
activities
analyzed
presence
exosome
treatment
alone
or
combination
with
altered
expression
NEAT1,
miR-144-3p
Rho-associated
(ROCK2).
miR-144-3p,
ROCK2,
corresponding
proteins
detected
RT-qPCR
western
blot
techniques.
binding
profile
NEAT1
evaluated
via
approach
luciferase
activity
assay,
RNA
immunoprecipitation,
pull-down
experiments.Blood
extracted
from
RA
patients
increased
incidence
destruction
significantly.
Overexpression
ROCK2
promoted
immune
(CD4+T
cells)
proliferation,
Th17
differentiation,
migration
response
stimulus,
whereas
knockout
gene
induced
cells.
exogenous
inhibited
inducing
activation
pathway.A
novel
NEAT1/miR-144-3p/ROCK2/WNT
investigated
potential
target
for
therapy.
Expert Reviews in Molecular Medicine,
Год журнала:
2022,
Номер
24
Опубликована: Янв. 1, 2022
Abstract
Rheumatoid
arthritis
(RA)
is
a
heterogeneous
autoimmune
disorder
that
leads
to
severe
joint
deformities,
negatively
affecting
the
patient's
quality
of
life.
Extracellular
vesicles
(EVs),
which
include
exosomes
and
ectosomes,
act
as
intercellular
communication
mediators
in
several
physiological
pathological
processes
various
diseases
including
RA.
In
contrast,
EVs
secreted
by
mesenchymal
stem
cells
perform
an
immunomodulatory
function
stimulate
cartilage
repair,
showing
promising
therapeutic
results
animal
models
from
other
sources,
dendritic
cells,
neutrophils
myeloid-derived
suppressor
also
influence
biological
immune
cells.
This
review
describes
role
pathogenesis
RA
presents
evidence
supporting
future
studies
on
potential
different
sources.
information
will
contribute
better
understanding
development,
well
starting
point
for
exploring
cell-free-based
therapies
Frontiers in Immunology,
Год журнала:
2022,
Номер
13
Опубликована: Фев. 15, 2022
Exosomes
are
nanoscale
extracellular
vesicles
(EVs),
which
present
in
all
body
fluids
tested.
They
secreted
by
a
variety
of
cells
including
macrophages,
dendritic
cells,
mast
granulocytes,
lymphocytes,
and
tumor
cells.
different
have
biological
components
functional
characteristics
play
an
important
role
many
pathophysiological
activities.
Recent
studies
revealed
that
exosomes
can
regulate
the
occurrence
development
inflammatory
immune
diseases
tumors
transmitting
their
unique
proteins,
lipids,
nucleic
acids
as
signaling
molecules
to
other
serve
novel
class
diagnostic
biomarkers
drug
delivery
systems
with
promising
applications
immunotherapy,
particularly
because
breakthroughs
nanotechnology
led
exploration
engineered
for
immunotargeted
therapies.
Therefore,
here
we
review
progress
being
made
on
application
immunotherapy
its
multiple
regulatory
mechanisms
explore
potential
future.
