Exosomes From Human Umbilical Cord Mesenchymal Stem Cells Treat Corneal Injury via Autophagy Activation DOI Creative Commons
Shisi Ma, Jiayang Yin, Lili Hao

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Апрель 11, 2022

Corneal injury (CI) affects corneal integrity and transparency, deteriorating the patient's quality of life. This study aimed to explore molecular mechanisms by which exosomes secreted from human umbilical cord mesenchymal stem cells (hucMSC-Exos) affect autophagy in epithelial (HCECs) CI models. We isolated identified hucMSC-Exos using nanoparticle tracking analysis, transmission electron microscopy, western blotting. The effects combined with regulators on HCECs mice were assessed cell viability assays, scratch assay, cycle apoptosis fluorescein staining, haze grades, pathological examinations, blotting, quantitative polymerase chain reaction (qPCR). In vitro results indicated that activator had positive promoting proliferation, migration capacity, upregulating proportions S phase expression PCNA, Cyclin A, E, CDK2. Meanwhile, combination treatment reduced apoptotic rate HCECs. vivo especially them significantly alleviated defects stromal opacity, levels markers Bax cleaved Caspase-3, inflammatory response products TNF-α, IL-1β, IL-6, CXCL-2, increased Bcl-2. was achieved pAMPK/AMPK pULK1/ULK1 ratios, Beclin-1 LC3B II/I, downregulating pmTOR/mTOR ratio p62. contrast, clinical indications, apoptosis, inflammation aggravated after application inhibitor. HucMSC-Exos an enhanced functions defects, activating signaling pathway, AMPK-mTOR-ULK1, providing a new biological therapy for wound healing ocular surface regeneration.

Язык: Английский

MicroRNAs of extracellular vesicles derived from mesenchymal stromal cells alleviate inflammation in dry eye disease by targeting the IRAK1/TAB2/NF-κB pathway DOI Creative Commons
Leying Wang, Xueyao Wang, Qiankun Chen

и другие.

The Ocular Surface, Год журнала: 2023, Номер 28, С. 131 - 140

Опубликована: Март 27, 2023

To investigate the efficacy and mechanisms of human umbilical cord-derived MSC-derived extracellular vesicles (hucMSC-EVs) in a mouse model desiccation-induced dry eye disease (DED).hucMSC-EVs were enriched by ultracentrifugation. The DED was induced desiccating environment combined with scopolamine administration. mice divided into hucMSC-EVs group, fluorometholone (FML) PBS blank control group. Tear secretion, corneal fluorescein staining, cytokine profiles tears goblet cells, TUNEL-positive cell, CD4+ cells examined to assess therapeutic efficiency. miRNAs sequenced, top 10 used for miRNA enrichment analysis annotation. targeted DED-related signaling pathway further verified using RT‒qPCR western blotting.Treatment increased tear volume maintained integrity mice. profile group presented lower level proinflammatory cytokines than Moreover, treatment cell density inhibited apoptosis infiltration. Functional showed high correlation immunity. Among them, miR-125 b, let-7b, miR-6873 conserved between humans associated IRAK1/TAB2/NF-κB that activated DED. Furthermore, activation abnormal expression IL-4, IL-8, IL-10, IL-13, IL-17, TNF-α reversed hucMSC-EVs.hucMSCs-EVs alleviate signs, suppress inflammation restore homeostasis surface multitargeting via certain miRNAs.

Язык: Английский

Процитировано

23
Extracellular vesicles in dry eye disease and Sjogren syndrome: A systematic review on their diagnostic and therapeutic role DOI Creative Commons
Prakshi Chopra,

Asra Fatima,

Sonali Mohapatra

и другие.

Survey of Ophthalmology, Год журнала: 2025, Номер 70(3), С. 499 - 515

Опубликована: Янв. 14, 2025

Язык: Английский

Процитировано

1
Application of Stem Cell-Derived Exosomes in Anterior Segment Eye Diseases: A Comprehensive Update Review DOI
Masoud Khorrami‐Nejad, Hesam Hashemian,

Ali Majdi

и другие.

The Ocular Surface, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Corneal Regeneration Using Adipose-Derived Mesenchymal Stem Cells DOI Creative Commons
Jorge L. Alió del Barrio, Ana de la Mata, María P. De Miguel

и другие.

Cells, Год журнала: 2022, Номер 11(16), С. 2549 - 2549

Опубликована: Авг. 16, 2022

Adipose-derived stem cells are a subtype of mesenchymal cell that offers the important advantage being easily obtained (in an autologous manner) from low invasive procedures, rendering high number multipotent with potential to differentiate into several cellular lineages, show immunomodulatory properties, and promote tissue regeneration by paracrine action through secretion extracellular vesicles containing trophic factors. This secretome is currently investigated as source for cell-free based regenerative therapy human tissues, which would significantly reduce involved costs, risks law regulations, allowing broader application in real clinical practice. In current article, we will review existing preclinical evidence regarding use such adipose-derived three main layers cornea: epithelium (derived surface ectoderm), stroma neural crest mesenchyme), endothelium cells).

Язык: Английский

Процитировано

37
Mesenchymal Stem Cell-Derived Exosomes in Ophthalmology: A Comprehensive Review DOI Creative Commons
Kevin Y. Wu, Hamza Ahmad, Grace Lin

и другие.

Pharmaceutics, Год журнала: 2023, Номер 15(4), С. 1167 - 1167

Опубликована: Апрель 6, 2023

Over the past decade, field of mesenchymal stem cell (MSC) therapy has exhibited rapid growth. Due to their regenerative, reparatory, and immunomodulatory capacities, MSCs have been widely investigated as therapeutic agents in cell-based treatment chronic ophthalmic pathologies. However, applicability MSC-based is limited by suboptimal biocompatibility, penetration, delivery target ocular tissues. An emerging body research elucidated role exosomes biological functions MSCs, that MSC-derived extracellular vesicles (EVs) possess anti-inflammatory, anti-apoptotic, tissue repairing, neuroprotective, properties similar MSCs. The recent advances MSCs-derived can serve solutions challenges faced MSCs-therapy. nano-dimensions, rapidly penetrate barriers reach immune-privileged organs, allowing for efficient factors such trophic tissues are typically challenging conventional transplantation. In addition, use EVs minimizes risks associated with this literature review, we focus on studies published between 2017 2022, highlighting characteristics derived from treating anterior posterior segment diseases. Additionally, discuss potential clinical settings. Rapid advancements regenerative medicine exosome-based drug delivery, conjunction an increased understanding pathology pharmacology, hold great promise therapies exciting revolutionize way approach these conditions.

Язык: Английский

Процитировано

19
Extracellular vesicles in degenerative retinal diseases: A new therapeutic paradigm DOI Creative Commons

Federico Manai,

Adrian Smędowski, Kai Kaarniranta

и другие.

Journal of Controlled Release, Год журнала: 2023, Номер 365, С. 448 - 468

Опубликована: Дек. 2, 2023

Nanoscale extracellular vesicles (EVs), consisting of exomers, exosomes and microvesicles/ectosomes, have been extensively investigated in the last 20 years, although their biological role is still something a mystery. EVs are involved transfer lipids, nucleic acids proteins from donor to recipient cells or distant organs as well regulating cell-cell communication signaling. Thus, important intercellular this not limited sister cells, but may also mediate crosstalk between different cell types even over long distances. play crucial functions both cellular homeostasis pathogenesis diseases, since contents reflect status cell, they represent an additional valuable source information for characterizing complex processes. Recent advances isolation analytical methods led substantial improvements engineering EVs, leading use either novel biomarkers disease diagnosis/prognosis therapies. Due capacity carry biomolecules, various EV-based therapeutic applications devised several pathological conditions, including eye diseases. In eye, detected retina, aqueous humor, vitreous body tears. Experiences with other forms intraocular drug opened new ways treatment retinal We here provide comprehensive summary main vitro, vivo, ex vivo literature-based studies on EVs' ocular physiological conditions. focused age-related macular degeneration, diabetic retinopathy, glaucoma, which common diseases permanent blindness, if treated properly. addition, putative retinitis pigmentosa retinopathies discussed. Finally, we reviewed potential tools and/or above-mentioned disorders. Evidence emerging experimental models human material strongly suggests future diagnostic exploitation these agents disorders good possibility improve patient's quality life.

Язык: Английский

Процитировано

18
NLRP3 Inflammasome as a Potential Therapeutic Target in Dry Eye Disease DOI Open Access
Dian Zhuang, Stuti L. Misra,

Odunayo O. Mugisho

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(13), С. 10866 - 10866

Опубликована: Июнь 29, 2023

Dry eye disease (DED) is a multifactorial ocular surface disorder arising from numerous interrelated underlying pathologies that trigger self-perpetuating cycle of instability, hyperosmolarity, and damage. Associated discomfort visual disturbance contribute negatively to quality life. Ocular inflammation has been increasingly recognised as playing key role in the pathophysiology chronic DED. Current readily available anti-inflammatory agents successfully relieve symptoms, but often without addressing pathophysiological mechanism. The NOD-like receptor protein-3 (NLRP3) inflammasome pathway recently implicated driver inflammation, reported pre-clinical clinical studies This review discusses intimate relationship between DED highlights involvement development DED, describes existing therapies their limitations, evaluates potential context therapeutic landscape target for effective treatment disease.

Язык: Английский

Процитировано

17
A synergistic therapeutic nano-eyedrop for dry eye disease based on ascorbic acid-coupled exosomes DOI Creative Commons
Fang Ma, Feng Jing, Xi Liu

и другие.

Nanoscale, Год журнала: 2022, Номер 15(4), С. 1890 - 1899

Опубликована: Дек. 16, 2022

Dry eye disease (DED), a complex ocular surface with high prevalence rate, is associated corneal injury, excess oxidative stress and inflammation. Current therapeutic strategies, including artificial tears anti-inflammatory agents, are unable to address all the deleterious factors or achieve clinical cure due their temporary side effects. Here, we prepared multiple-functional eyedrop based on deposition of gold nanoparticles (AuNPs) reduced by ascorbic acid (AA) onto exosomal phospholipid membrane mesenchymal stem cell (mExo)-derived exosomes in situ (mExo@AA). The value mExo@AA for DED was demonstrated mouse model. Combining benefits mExo AA, effectively improves epithelium recovery anti-inflammation capacity, decreases reactive oxygen species, restores tear secretion without adverse Thus, this study suggests that effective safe as agent treatment DED.

Язык: Английский

Процитировано

24
BMSC-derived exosomes regulate the Treg/Th17 balance through the miR-21-5p/TLR4/MyD88/NF-κB pathway to alleviate dry eye symptoms in mice DOI
Dandan Zhao, Hao Ji, Hongxia Zhao

и другие.

In Vitro Cellular & Developmental Biology - Animal, Год журнала: 2024, Номер 60(6), С. 644 - 656

Опубликована: Апрель 29, 2024

Язык: Английский

Процитировано

5
Advances in Extracellular-Vesicles-Based Diagnostic and Therapeutic Approaches for Ocular Diseases DOI Creative Commons
Yun Su,

Moxin Chen,

Wei Xu

и другие.

ACS Nano, Год журнала: 2024, Номер 18(34), С. 22793 - 22828

Опубликована: Авг. 14, 2024

Extracellular vesicles (EVs) are nanoscale membrane of various sizes that can be secreted by most cells. EVs contain a diverse array cargo, including RNAs, lipids, proteins, and other molecules with functions intercellular communication, immune modulation, regulation physiological pathological processes. The biofluids in the eye, tears, aqueous humor, vitreous important sources for EV-based diagnosis ocular disease. Because molecular cargos may reflect biology their parental cells, these biofluids, as well blood, have been recognized promising candidates biomarkers early Moreover, also used therapeutics targeted drug delivery nanocarriers many disorders because low immunogenicity superior biocompatibility nature. In this review, we provide an overview recent advances field studies on We summarized origins applied disease, assessed different methods EV isolation from biofluid samples, highlighted bioengineering strategies systems, introduced latest applications treatment presented potential current clinical trials. Finally, briefly discussed challenges disease some issues concern better focusing translational future.

Язык: Английский

Процитировано

5