Journal of Neurochemistry, Год журнала: 2024, Номер unknown

Опубликована: Авг. 17, 2024

Abstract Myelin is a vital structure that key to rapid saltatory conduction in the central and peripheral nervous systems. Much work has been done over decades examining biochemical composition morphology of myelin at light electron microscopic levels. Here we report method study based on fluorescent probe Nile Red. This lipophilic dye readily partitions into live chemicallyfixed producing bright, well‐resolved images sheath. Using spectral confocal microscopy, complete emission spectrum Red fluorescence can be acquired for each pixel an image. The solvatochromic properties cause its change depending polarity local environment. Therefore, measuring shifts subtle changes physicochemical myelin. We show differences versus system different regions white matter mouse brain, together with developmental sex variations. technique also well suited ex vivo specimens. demonstrate how deprivation induces adult optic nerve underscoring continuing plasticity response axonal activity adulthood. spectroscopic allows measurement importantly influence electrical by extension, velocities axons. image

Язык: Английский