High-throughput Mucus Microrheology for Phenotyping and Disease Modeling
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 14, 2025
Mucus
plays
an
integral
role
for
the
barrier
function
of
many
epithelial
tissues.
In
human
airways,
mucus
is
constantly
secreted
to
capture
inhaled
microbes
and
pollutants
cleared
away
through
concerted
ciliary
motion.
Many
important
respiratory
diseases
exhibit
altered
flowability
impaired
clearance,
contributing
distress
increased
risk
infections.
Understanding
how
rheology
changes
during
disease
progression
in
response
treatments
thus
great
interest
subtyping
patients
tailoring
treatments,
probing
mechanisms,
therapies;
however,
basic
research
greatly
hampered
by
lack
scalable
user-friendly
rheometry
assays
small
volumes
typically
produced
vitro
models
clinical
ex
vivo
settings.
To
address
this
challenge,
we
developed
a
streamlined,
high-throughput
protocol
leveraging
Differential
Dynamic
Microscopy
(DDM)
reliably
measure
frequency-dependent
microrheology
minuscule
(3-10
µ
L)
samples
using
standard
epifluorescence
microscopy.
Our
method
does
not
require
time-consuming
user-interventions
common
particle
tracking
routines
measures
at
time
scale
relaxation
(1-20s),
hence
reducing
assay
time.
We
demonstrate
successful
application
our
harvested
from
state-of-art
air-liquid-interface
(ALI)
cultures
assess
airway
different
culture
conditions.
show
that
approach
equally
applies
other
types
sources
mucus,
also
validated
cervical
mucus.
envision
can
be
seamlessly
adopted
non-expert
users,
without
need
specialized
equipment
or
extensive
training,
study
their
respiratory,
intestinal,
reproductive
mucosal
organ
systems.
This
advancement
opens
up
new
avenues
large-scale
studies,
providing
insights
into
previously
limited
data
accessibility
resource
constraints.
Язык: Английский
Interplay between respiratory viruses and cilia in the airways
European Respiratory Review,
Год журнала:
2025,
Номер
34(175), С. 240224 - 240224
Опубликована: Янв. 1, 2025
The
airway
epithelium
is
the
first
point
of
contact
for
inhaled
pathogens.
role
epithelial
cells
in
clearance,
infection
and
colonisation
bacteria
established.
interactions
respiratory
viruses
cilia
less
understood,
but
are
known
to
target
ciliated
entry,
replication
dissemination.
Furthermore,
some
impair
and/or
enhance
ciliary
activity.
This
review
examines
what
about
between
viral
how
effect
function
with
subsequent
consequences
human
health.
We
discuss
models
which
can
be
used
investigate
relationship
host
airway.
Язык: Английский
Helicobacter Pylori's Involvement in the Occurrence and Development of Vocal Fold Leukoplakia Through the Nrf2/Keap1 Pathway
Опубликована: Янв. 1, 2025
Язык: Английский
Culture Conditions Differentially Regulate the Inflammatory Niche and Cellular Phenotype of Tracheo-Bronchial Basal Stem Cells
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 5, 2024
ABSTRACT
Human
bronchial
epithelial
cells
(HBECs)
derived
from
the
tracheo-bronchial
regions
of
human
airways
provide
an
excellent
in
vitro
model
for
studying
pathological
mechanisms
and
evaluating
therapeutics
airway
cells.
This
cell
population
comprises
a
mixed
basal
(BCs),
predominant
stem
capable
both
self-renewal
functional
differentiation.
Despite
their
potential
regenerative
medicine,
BCs
exhibit
significant
phenotypic
variability
culture.
To
investigate
how
culture
conditions
influence
BC
phenotype
function,
we
expanded
three
independent
isolates
media,
growth
medium
(AECGM),
dual-SMAD
inhibitor
(DSI)-enriched
AECGM,
Pneumacult
Ex
plus
(PEx+).
Extensive
RNA
sequencing,
immune
assays
electrical
measurements
revealed
that
PEx+
media
significantly
drove
proliferation
broad
pro-inflammatory
BCs.
In
contrast,
displayed
increased
expression
structural
extracellular
matrix
components
at
high
passage.
Whereas
AECGM
some
cytokines
passage,
DSI
suppressed
inflammation
altogether
thus
implicating
TGF-β
inflammatory
processes.
Differentiation
capacity
declined
with
time
irrespective
expansion
except
PLUNC
expressing
secretory
were
elevated
passage
suggestive
modulatory
role
These
findings
underscore
profound
impact
on
niche
function
The
driven
by
particular,
should
be
considered
development
cell-based
models
diseases
therapeutic
application.
NEW
&
NOTEWORTHY
Airway
cells,
vital
regeneration
therapies,
show
changes
based
conditions.
Our
study
reveals
composition
duration
greatly
affect
properties
deposition
results
critical
phenotype,
influencing
disease
research
therapy.
Язык: Английский
Airway epithelium in lung transplantation: a potential actor for post-transplant complications?
European Respiratory Review,
Год журнала:
2024,
Номер
33(174), С. 240093 - 240093
Опубликована: Окт. 1, 2024
Lung
transplantation,
a
critical
intervention
for
end-stage
lung
diseases,
is
frequently
challenged
by
post-transplant
complications.
Indeed,
primary
graft
dysfunction,
anastomotic
complications,
infections
and
acute
chronic
rejections
pose
significant
hurdles
in
transplantation.
While
evidence
regarding
the
role
of
airway
epithelium
after
transplantation
still
emerging,
its
importance
becoming
increasingly
recognised.
This
review
looks
at
complex
involvement
various
while
emphasising
utility
epithelial
culture
as
research
model.
In
summary,
elucidating
each
complication
explaining
these
intricate
processes,
aims
to
guide
specific
future
efforts
therapeutic
strategies
aimed
improving
transplant
outcomes
enhancing
patient
care.
Язык: Английский