Meta-analysis of retinal transcriptome profiling studies in animal models of myopia DOI Creative Commons
Teele Palumaa,

Shruti Balamurugan,

Machelle T. Pardue

и другие.

Frontiers in Medicine, Год журнала: 2025, Номер 11

Опубликована: Янв. 14, 2025

Objective Myopia prevalence is increasing at alarming rates, yet the underlying mechanistic causes are not understood. Several studies have employed experimental animal models of myopia and transcriptome profiling to identify genes pathways contributing myopia. In this study, we determined retinal changes in response form deprivation mouse retinas. We then conducted a meta-analysis incorporating all publicly available datasets analyzed how results related associated with refractive errors human genome-wide association (GWAS). Methods Form was induced three male C57BL6/J mice from postnatal day 28 (P28) P42. Retinal gene expression RNA sequencing, followed by differential analysis DESeq2 identification Kyoto Encyclopedia Genes Genomes (KEGG). A systematic search identified four similar transcriptomics using chicks or mice. The five underwent meta-analyses determine pathways. were compared Results Differential form-deprived retinas revealed 235 significantly altered transcripts, implicating BMP2 signaling pathway circadian rhythms, among others. Transcriptome-wide found 427 differentially expressed model 1,110 chick model, limited overlap between species. Pathway these two sets implicated TGF-beta rhythm both Some only included dopamine HIF-1 pathway, whereas glucagon follistatin changed has also been entrainment processes mice, chicks, humans. Conclusion This study highlights power combining enhance statistical robust across different conditions. data supports other evidence that rhythms involved myopic eye growth.

Язык: Английский

Meta-analysis of retinal transcriptome profiling studies in animal models of myopia DOI Creative Commons
Teele Palumaa,

Shruti Balamurugan,

Machelle T. Pardue

и другие.

Frontiers in Medicine, Год журнала: 2025, Номер 11

Опубликована: Янв. 14, 2025

Objective Myopia prevalence is increasing at alarming rates, yet the underlying mechanistic causes are not understood. Several studies have employed experimental animal models of myopia and transcriptome profiling to identify genes pathways contributing myopia. In this study, we determined retinal changes in response form deprivation mouse retinas. We then conducted a meta-analysis incorporating all publicly available datasets analyzed how results related associated with refractive errors human genome-wide association (GWAS). Methods Form was induced three male C57BL6/J mice from postnatal day 28 (P28) P42. Retinal gene expression RNA sequencing, followed by differential analysis DESeq2 identification Kyoto Encyclopedia Genes Genomes (KEGG). A systematic search identified four similar transcriptomics using chicks or mice. The five underwent meta-analyses determine pathways. were compared Results Differential form-deprived retinas revealed 235 significantly altered transcripts, implicating BMP2 signaling pathway circadian rhythms, among others. Transcriptome-wide found 427 differentially expressed model 1,110 chick model, limited overlap between species. Pathway these two sets implicated TGF-beta rhythm both Some only included dopamine HIF-1 pathway, whereas glucagon follistatin changed has also been entrainment processes mice, chicks, humans. Conclusion This study highlights power combining enhance statistical robust across different conditions. data supports other evidence that rhythms involved myopic eye growth.

Язык: Английский

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