Follicle-stimulating hormone promotes EndMT in endothelial cells by upregulating ALKBH5 expression

Cellular & Molecular Biology Letters, Год журнала: 2025, Номер 30(1)

Опубликована: Апрель 4, 2025

Abstract Background The incidence of atherosclerosis markedly rises following menopause. Our previous findings demonstrated that elevated follicle-stimulating hormone (FSH) levels in postmenopausal women accelerate progression. Plaque instability, the fundamental pathological factor acute coronary syndrome, primarily results from vascular embolism due to plaque rupture. Recent evidence highlights endothelial-to-mesenchymal transition (EndMT) exacerbates although link between FSH and EndMT has not been fully established. This investigation sought explore possible influence modulating EndMT. Methods In this study, apolipoprotein E -deficient ( ApoE −/− ) mice served as an model, while human umbilical endothelial cells (HUVECs) were used cellular models. Protein assessed through immunochemical techniques, gene expression was quantified via RT-qPCR, nucleic acid–protein interactions evaluated using immunoprecipitation. m6A modification status determined by MeRIP, behaviors analyzed standard biochemical assays. Results indicate induces both vitro vivo. Additional suggested upregulates transcription Forkhead box protein M1 (FOXM1) at mRNA enhancing AlkB homolog 5, RNA demethylase (ALKBH5). reduces modifications on FOXM1 ALKBH5, leading increased nascent transcript stability . Dual-luciferase reporter assays highlighted cAMP-response element binding (CREB)’s essential function facilitating FSH-induced upregulation ALKBH5. Conclusions These suggest promotes ALKBH5 expression, facilitates N 6 -methyladenosine (m6A) demethylation , consequently, study elucidates impact instability provides insights into potential strategies prevent syndrome women. Graphical

Язык: Английский

Topical application of Cap-loaded hydrogels inhibits corneal neovascularization

Experimental Eye Research, Год журнала: 2025, Номер unknown, С. 110390 - 110390

Опубликована: Апрель 1, 2025

Язык: Английский