Dysregulated Mechanotransduction via CCNB2 Underlies Fibroblast Hypoplasia in Developmental Dysplasia of the Hip DOI
Yu Rao, An Min Wang, Lili Yang

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Abstract Purpose: Developmental dysplasia of the hip (DDH) involves structural and functional impairments in joint capsule; however, molecular mechanisms underlying these changes remain unclear. This study aimed to identify biomarkers associated with fibroblast mechanical signaling capsule patients DDH explore their potential roles disease pathogenesis. Methods: Joint tissues were collected from age‐ sex‐matched healthy controls. RNA sequencing (RNA-seq) identified differentially expressed cell-cell communication-related genes (DE-CMRGs). Five bioinformatics algorithms (MCC, MNC, Degree, Closeness, Eccentricity) applied key DE-CMRGs correlated function. Biomarker validation was performed using reverse transcription quantitative PCR (RT-qPCR), Western blot, immunohistochemistry (IHC), hematoxylin-eosin (HE) staining. Additionally, immune infiltration analysis regulatory network construction (TF-mRNA lncRNA-miRNA-mRNA ceRNA networks) conducted elucidate mechanisms. Results: Transcriptomic revealed 155 DE-CMRGs, which five (KIF20A, BUB1, CCNB2, AURKB, BUB1B) identified. These significantly downregulated samples. Functional enrichment analyses highlighted involvement pathways related muscle contraction, extracellular matrix interactions, cell cycle regulation, responses. Immune showed correlations between specific populations, particularly memory-activated CD4 T cells, macrophages, neutrophils, dendritic monocytes. Experimental confirmed that CCNB2 at both mRNA protein levels compared Histopathological examinations reduced density altered cellular architecture decreased expression. Conclusion: This identifies validates novel biomarkers, communication fibroblasts DDH. together networks, offer new insights into pathogenesis highlight targets for early diagnosis therapeutic intervention.

Язык: Английский

Dysregulated Mechanotransduction via CCNB2 Underlies Fibroblast Hypoplasia in Developmental Dysplasia of the Hip DOI
Yu Rao, An Min Wang, Lili Yang

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Abstract Purpose: Developmental dysplasia of the hip (DDH) involves structural and functional impairments in joint capsule; however, molecular mechanisms underlying these changes remain unclear. This study aimed to identify biomarkers associated with fibroblast mechanical signaling capsule patients DDH explore their potential roles disease pathogenesis. Methods: Joint tissues were collected from age‐ sex‐matched healthy controls. RNA sequencing (RNA-seq) identified differentially expressed cell-cell communication-related genes (DE-CMRGs). Five bioinformatics algorithms (MCC, MNC, Degree, Closeness, Eccentricity) applied key DE-CMRGs correlated function. Biomarker validation was performed using reverse transcription quantitative PCR (RT-qPCR), Western blot, immunohistochemistry (IHC), hematoxylin-eosin (HE) staining. Additionally, immune infiltration analysis regulatory network construction (TF-mRNA lncRNA-miRNA-mRNA ceRNA networks) conducted elucidate mechanisms. Results: Transcriptomic revealed 155 DE-CMRGs, which five (KIF20A, BUB1, CCNB2, AURKB, BUB1B) identified. These significantly downregulated samples. Functional enrichment analyses highlighted involvement pathways related muscle contraction, extracellular matrix interactions, cell cycle regulation, responses. Immune showed correlations between specific populations, particularly memory-activated CD4 T cells, macrophages, neutrophils, dendritic monocytes. Experimental confirmed that CCNB2 at both mRNA protein levels compared Histopathological examinations reduced density altered cellular architecture decreased expression. Conclusion: This identifies validates novel biomarkers, communication fibroblasts DDH. together networks, offer new insights into pathogenesis highlight targets for early diagnosis therapeutic intervention.

Язык: Английский

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