Immunohistochemical Profiling of Histone Modification Biomarkers Identifies Subtype-Specific Epigenetic Signatures and Potential Drug Targets in Breast Cancer DOI

Zirong Huo,

Sitong Zhang, Guodong Su

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Abstract Background: Breast cancer (BC) subtypes exhibit distinct epigenetic landscapes, with triple-negative breast (TNBC) lacking effective targeted therapies. This study investigates histone biomarkers and therapeutic vulnerabilities across BC subtypes. Methods: Immunohistochemical profiling of >20 biomarkers, including modifications, modifiers oncohistone mutations was conducted on a discovery cohort validation tissues, healthy controls cell line models. Transcriptomic growth analyses were to evaluate the effects small-molecule G9a inhibitor in diverse Results: Key H3K9me2, H3K36me2, H3K79me, differentially expressed H3K9me2 emerged as an independent predictor for distinguishing TNBC from other less aggressive subtypes, elevated expression correlating higher tumor grade stage. inhibition impaired proliferation modulated epithelial-mesenchymal transition pathways, strongest impact basal-like TNBC. Disruption oncogene suppressor regulation (e.g., TP53, SATB1) observed Conclusion: highlights G9a’s context-dependent roles BC, supporting its potential target. Findings provide foundation subtype-specific therapies improve outcomes Clinical Perspective undertaken explore landscape focusing modifications their potential, particularly through targeting G9a, methyltransferase. The identified emerging key marker demonstrated robust anti-cancer effects, impairment disruption oncogenic These findings compelling evidence development similar regulators, highlighting reshape treatment patient outcomes.

Язык: Английский

Safety profile of EZH2 inhibitors for cancer: a systematic review and meta-analysis DOI Creative Commons
Zhou Zhao, Xiufeng Chen,

Hua‐Yang Pang

и другие.

PeerJ, Год журнала: 2025, Номер 13, С. e18871 - e18871

Опубликована: Янв. 27, 2025

To evaluate the safety profiles of EZH2-targeted inhibitors in cancer treatment, focusing on treatment-related adverse events (TRAEs) across various clinical trials. We conducted a systematic review and meta-analysis using data from trials involving EZH2 reported up to May 31, 2024. Databases searched included PubMed, Embase, CENTRAL (Cochrane Central Register Controlled Trials), ClinicalTrials.gov. Studies were those patients treated with as monotherapy or combination, specifically detailing incidence TRAEs. Data all-grade TRAEs, grade 3 higher severe TRAEs extracted analyzed random-effects models. Our 22 studies encompassing 1,002 who met inclusion criteria. commonly observed during inhibitor therapy, affecting 86% (95% CI [79-94%]%; I2 = 89.5%). The was 33% [21-44%]; 93.5%), while occurred 15% cases [9-22%]; 87.5%). most frequently pooled analysis neutropenia (8%), thrombocytopenia anemia (6%). Specifically, for tazemetostat, common TRAE (5%). For SHR2554, prevalent (17%), (7%). Notably, fatalities rare, only 0.9% experiencing potentially fatal outcomes due therapy. demonstrate manageable profile low emphasizing their potential safe therapeutic options treatment. rate rare occurrences deaths support continued use further investigation inhibitors.

Язык: Английский

Процитировано

0

Immunohistochemical Profiling of Histone Modification Biomarkers Identifies Subtype-Specific Epigenetic Signatures and Potential Drug Targets in Breast Cancer DOI Open Access

Zirong Huo,

Sitong Zhang, Guodong Su

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 770 - 770

Опубликована: Янв. 17, 2025

Breast cancer (BC) subtypes exhibit distinct epigenetic landscapes, with triple-negative breast (TNBC) lacking effective targeted therapies. This study investigates histone biomarkers and therapeutic vulnerabilities across BC subtypes. The immunohistochemical profiling of >20 biomarkers, including modifications, modifiers, oncohistone mutations, was conducted on a discovery cohort validation tissues, healthy controls, cell line models. Transcriptomic growth analyses were to evaluate the effects small-molecule G9a inhibitor in diverse Key H3K9me2, H3K36me2, H3K79me, differentially expressed H3K9me2 emerged as an independent predictor for distinguishing TNBC from other less-aggressive subtypes, elevated expression correlating higher tumor grade stage. inhibition impaired proliferation modulated epithelial–mesenchymal transition pathways, strongest impact basal-like TNBC. disruption oncogene suppressor regulation (e.g., TP53, SATB1) observed highlights G9a’s context-dependent roles BC, supporting its potential target. findings provide foundation subtype-specific therapies improve outcomes aggressive

Язык: Английский

Процитировано

0

A narrative review of epigenetic marker in H3K27ac and its emerging potential as a therapeutic target in cancer DOI

Meizi Zhu,

Xuejin Lu,

Danhong Wang

и другие.

Epigenomics, Год журнала: 2025, Номер unknown, С. 1 - 17

Опубликована: Фев. 21, 2025

Histone acetylation, particularly H3 K27 acetylation (H3K27ac), is a critical post-translational modification that regulates chromatin structure and gene expression, which plays significant role in various cancers, including breast, colon, lung, hepatocellular, prostate cancer. However, the mechanisms of H3K27ac tumorigenesis are not yet comprehensive, especially its epigenetic mechanisms. This review endeavors to discuss findings on involvement carcinogenesis within past 5 years through literature search using academic databases such as Web Science. Firstly, we provide an overview diverse landscape histone modifications, emphasizing distinctive characteristics significance H3K27ac. Secondly, summarize compare advanced high-throughput sequencing technologies have been utilized construction map. Thirdly, elucidate mediating transcription. Fourthly, venture into potential molecular mechanism cancer development. Finally, engage discussing future therapeutic approaches oncology, with spotlight strategies harness H3K27 modifications. In conclusion, this comprehensively summarizes underscores pivotal cancer, providing valuable insights target for intervention.

Язык: Английский

Процитировано

0

Exploring Mechanisms of Action in Combinatorial Therapy through Stability/Solubility Alterations: Advancing AML Treatment DOI Creative Commons
Elham Gholizadeh, Ehsan Zangene, Uladzislau Vadadokhau

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 8, 2024

Abstract Acute myeloid leukemia (AML) is an aggressive blood cancer with a poor prognosis. Although treatments like allogeneic hematopoietic stem cell transplantation and high-dose chemotherapy can potentially cure younger patients in some cases, challenges such as relapse treatment-related toxicities remain significant. Combination therapy has been cornerstone AML treatment, offering enhanced efficacy by leveraging the synergistic effects of multiple agents. However, high toxicity levels genetic heterogeneity complicate identification effective universally applicable drug regimens. To address these challenges, we introduce CoPISA workflow (Proteome Integral Solubility/Stability Alteration Analysis for Combinations), innovative method designed to study drug-target interactions specifically within combination therapies. detects changes protein solubility/stability that occur only when two drugs are used together, revealing cooperative mechanisms single-drug cannot achieve. We applied this highly low-toxicity combinations AML, previously introduced our group: LY3009120-sapanisertib (LS) ruxolitinib-ulixertinib (RU). utilizes advanced proteomic tools investigate both primary secondary target effects, providing deeper understanding how therapies influence signaling pathways overcome resistance. Furthermore, propose novel concept termed “conjunctional inhibition”, where combined action induces biological responses be achieved individual This approach introduces transformation designing combinatorial offers new directions more other complex diseases.

Язык: Английский

Процитировано

0

Immunohistochemical Profiling of Histone Modification Biomarkers Identifies Subtype-Specific Epigenetic Signatures and Potential Drug Targets in Breast Cancer DOI

Zirong Huo,

Sitong Zhang, Guodong Su

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Abstract Background: Breast cancer (BC) subtypes exhibit distinct epigenetic landscapes, with triple-negative breast (TNBC) lacking effective targeted therapies. This study investigates histone biomarkers and therapeutic vulnerabilities across BC subtypes. Methods: Immunohistochemical profiling of >20 biomarkers, including modifications, modifiers oncohistone mutations was conducted on a discovery cohort validation tissues, healthy controls cell line models. Transcriptomic growth analyses were to evaluate the effects small-molecule G9a inhibitor in diverse Results: Key H3K9me2, H3K36me2, H3K79me, differentially expressed H3K9me2 emerged as an independent predictor for distinguishing TNBC from other less aggressive subtypes, elevated expression correlating higher tumor grade stage. inhibition impaired proliferation modulated epithelial-mesenchymal transition pathways, strongest impact basal-like TNBC. Disruption oncogene suppressor regulation (e.g., TP53, SATB1) observed Conclusion: highlights G9a’s context-dependent roles BC, supporting its potential target. Findings provide foundation subtype-specific therapies improve outcomes Clinical Perspective undertaken explore landscape focusing modifications their potential, particularly through targeting G9a, methyltransferase. The identified emerging key marker demonstrated robust anti-cancer effects, impairment disruption oncogenic These findings compelling evidence development similar regulators, highlighting reshape treatment patient outcomes.

Язык: Английский

Процитировано

0