Transcriptome Reveals the Differential Regulation of Sugar Metabolism to Saline–Alkali Stress in Different Resistant Oats

Genes, Год журнала: 2025, Номер 16(1), С. 105 - 105

Опубликована: Янв. 20, 2025

Background: Saline–alkali stress is a major factor limiting the growth of oats. Sugar primary carbon and energy source in plants which regulates plant development by regulating enzyme activity gene expression. Sucrose, glucose, fructose are ubiquitous plant-soluble sugars that act as signalling molecules transcriptional regulation various metabolic defence-related genes. Methods: In this study, soluble sugars, fructose, sucrose, starch contents were measured, transcriptomics was used to determine differentially expressed genes (DEGs) saline-sensitive saline-tolerant oats after 6, 12, 24, 48 h. DEGs annotated carbohydrates selected using Kyoto Encyclopedia Genes Genomes. Results: involved carbohydrate metabolism mainly enriched glycolysis/gluconeogenesis pentose phosphate pathways, mannose metabolism, sucrose metabolism. GAPDH, SUPI, SUS2, ATP-PEK, HXK6, FBA4, TBA4, TKT, ISA3, PPDK1, BAM2 significantly expressed, quantitative reverse transcription polymerase chain reaction verified transcriptome sequencing results. Conclusions: with different salinity tolerances sugar under four durations, explore regulatory mechanism provide reference for elucidating abiotic stress.

Язык: Английский

Genomic Analysis of Hexokinase Genes in Foxtail Millet (Setaria italica): Haplotypes and Expression Patterns Under Abiotic Stresses

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1962 - 1962

Опубликована: Фев. 24, 2025

Hexokinases (HXKs) in plants are multifunctional enzymes that not only phosphorylate hexose but also function as glucose sensors, integrating nutrient, light, and hormone signaling networks to regulate cell metabolism pathways, thereby controlling growth development response environmental changes. To date, limited information is available regarding the HXKs of foxtail millet (Setaria italica L.). In this study, six HXK genes were identified characterized millet. Phylogenetic analysis revealed hexokinases classified into three subfamilies, corresponding two types (B-type C-type) plants. Gene structure conserved motif showed SiHXKs exhibited varying numbers introns exons, with proteins each subfamily showing similar organization. Evolutionary divergence indicated green families underwent both positive negative selection experienced a large-scale duplication event approximately 1.18-154.84 million years ago. Expression these widely expressed roots, stems, leaves, panicles, anthers, seeds, most significantly increased expression roots under abiotic stress conditions, including 20% PEG 6000 (drought stress), 200 μmol/L NaCl (salt 1 BR (brassinosteroid-mediated response). These results suggest may play pivotal role enhancing tolerance. Subcellular localization assay SiHXK5 SiHXK6 predominantly localized mitochondria. Haplotype SiHXK3-H1 was associated higher plant height grain yield. findings provide valuable insights functional characteristics genes, especially context marker-assisted pyramiding advantageous haplotypes breeding programs.

Язык: Английский

Transcriptome analysis reveals a regulatory role of hexokinase in kiwifruit postharvest softening

LWT, Год журнала: 2025, Номер unknown, С. 117717 - 117717

Опубликована: Март 1, 2025

Язык: Английский