International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(14), С. 11581 - 11581
Опубликована: Июль 18, 2023
The
treatment
of
chondral
and
osteochondral
defects
is
challenging.
These
types
lesions
are
painful
progress
to
osteoarthritis
over
time.
Tissue
engineering
offers
tools
address
this
unmet
medical
need.
use
an
autologous
cartilage
construct
consisting
hyaline
chips
embedded
in
plasma
rich
growth
factors
(PRGF)
has
been
proposed
as
a
therapeutic
alternative.
purpose
study
was
dig
into
the
potential
mechanisms
behind
vitro
remodelling
process
that
might
explain
clinical
success
technique
facilitate
its
optimisation.
Chondrocyte
viability
cellular
behaviour
eight
weeks
culture,
type
II
collagen
synthesis,
dual
delivery
by
PRGF
matrix,
ultrastructure
were
characterised.
main
finding
research
fragments
three-dimensional
scaffold
contain
viable
chondrocytes
able
migrate
fibrin
network,
proliferate
synthesise
extracellular
matrix
after
second
week
culture.
characterization
key
unravelling
molecular
kinetics
responsible
for
efficacy.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Июнь 26, 2024
Abstract
Tissue
regeneration
technology
has
been
rapidly
developed
and
widely
applied
in
tissue
engineering
repair.
Compared
with
traditional
approaches
like
surgical
treatment,
the
rising
gene
therapy
is
able
to
have
a
durable
effect
on
regeneration,
such
as
impaired
bone
articular
cartilage
repair
cancer-resected
Gene
can
also
facilitate
production
of
situ
therapeutic
factors,
thus
minimizing
diffusion
or
loss
complexes
enabling
spatiotemporally
controlled
release
products
for
regeneration.
Among
different
delivery
vectors
supportive
gene-activated
matrices,
advanced
gene/drug
nanocarriers
attract
exceptional
attraction
due
their
tunable
physiochemical
properties,
well
excellent
adaptive
performance
bone,
cartilage,
blood
vessel,
nerve
This
paper
reviews
recent
advances
nonviral-mediated
systems
an
emphasis
important
role
Abstract
Destruction
of
cartilage
due
to
the
abnormal
remodeling
subchondral
bone
(SB)
leads
osteoarthritis
(OA),
and
restoring
chondro‐bone
metabolic
homeostasis
is
key
treatment
OA.
However,
traditional
intra‐articular
injections
for
OA
cannot
directly
break
through
barrier
reach
SB.
In
this
study,
hydrothermal
method
used
synthesize
ultra‐small
size
(≈5
nm)
selenium‐doped
carbon
quantum
dots
(Se‐CQDs,
SC),
which
conjugated
with
triphenylphosphine
(TPP)
create
TPP‐Se‐CQDs
(SCT).
Further,
SCT
dynamically
complexed
hyaluronic
acid
modified
aldehyde
methacrylic
anhydride
(AHAMA)
construct
highly
permeable
micro/nano
hydrogel
microspheres
(SCT@AHAMA)
homeostasis.
vitro
experiments
confirmed
that
selenium
atoms
scavenged
reactive
oxygen
species
(ROS)
from
mitochondria
mononuclear
macrophages,
inhibited
osteoclast
differentiation
function,
suppressed
early
chondrocyte
apoptosis
maintain
a
balance
between
matrix
synthesis
catabolism.
vivo
further
demonstrated
delivery
system
osteoclastogenesis
H‐vessel
invasion,
thereby
regulating
initiation
process
inhibiting
degeneration
in
conclusion,
based
on
facilitate
efficient
penetration
articular
SB
regulate
metabolism
treatment.
Biomedicines,
Год журнала:
2024,
Номер
12(6), С. 1367 - 1367
Опубликована: Июнь 19, 2024
Chondrocyte-based
cell
therapy
has
been
used
for
more
than
30
years
and
is
still
considered
to
be
a
promising
method
of
cartilage
repair
despite
some
limitations.
This
review
introduces
the
latest
developments
four
generations
autologous
chondrocyte
implantation
current
products.
The
regeneration
from
adult
chondrocytes
limited
by
culture-induced
dedifferentiation
patient
age.
Cartibeads
an
innovative
three-step
produce
high-quality
hyaline
microtissues,
it
developed
dedifferentiated
with
high
number
passages.
In
addition,
allogeneic
therapies
using
Quantum
hollow-fiber
bioreactor
several
signaling
pathways
involved
in
chondrocyte-based
are
mentioned,
such
as
WNT
signaling,
BMP-2/WISP1
pathway,
FGF19
pathway.
ACS Applied Bio Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 17, 2025
The
cartilage
possesses
limited
regenerative
capacity,
necessitating
advanced
approaches
for
its
repair.
This
study
introduces
a
bioink
designed
tissue
engineering
(TE)
by
incorporating
ionically
cross-linkable
alginate
into
the
photo-cross-linkable
MuMA
bioink,
resulting
in
double
cross-linked
interpenetrating
network
(IPN)
hydrogel.
Additionally,
hyaluronic
acid
(HA),
natural
component
of
and
synovial
fluid,
was
added
to
enhance
scaffold's
properties.
HA
has
been
demonstrated
improve
lubrication,
regulate
inflammation,
promote
cell
proliferation,
support
extracellular
matrix
(ECM)
deposition
regeneration,
making
it
valuable
TE.
Comprehensive
experiments
were
conducted
assess
morphology,
swelling,
degradation,
mechanical
rheological
properties,
printability,
biocompatibility.
Results
indicated
that
scaffolds
comprising
MuMA,
alginate,
exhibited
compressive
moduli
comparable
native
cartilage,
unlike
single
variants.
cross-linking
also
influenced
water
uptake,
porosity,
contributing
scaffold
durability
stability
chondrocyte
support.
Biocompatibility
tests
with
C28/I2
cells
cell-supportive
chondrogenic
potential
bioink.
establishes
mucin
as
versatile
material
specialized
applications.
Osteoarthritis
(OA)
is
the
most
prevalent
joint
disease,
yet
effective
disease-modifying
OA
drugs
(DMOADs)
remain
elusive.
Targeting
macrophage
polarization
has
emerged
as
a
promising
avenue
for
treatment.
This
study
identified
skatole
through
high-throughput
screening
an
efficient
modulator
of
polarization.
In
vivo
experiments
demonstrated
that
administration
markedly
reduced
synovitis
and
cartilage
damage
in
both
destabilization
medial
meniscus
(DMM)-induced
mice
monosodium
iodoacetate
(MIA)-induced
rats.
Mechanistically,
activated
signal
transducer
activator
transcription
6
(Stat6)
signaling,
promoting
M2
polarization,
while
inhibiting
nuclear
factor-κB
(NFκB)
mitogen-activated
protein
kinase
(MAPK)
signaling
pathways
to
suppress
M1
RNA-sequencing
analysis,
targeted
metabolomics,
mitochondrial
stress
tests
further
revealed
treatment
shifted
macrophages
toward
oxidative
phosphorylation
energy
production.
Additionally,
it
up-regulated
genes
associated
with
glutathione
metabolism
reactive
oxygen
species
(ROS)
pathways,
reducing
intracellular
ROS
The
CUT&Tag
assay
results
indicated
downstream
factor
p65
NFκB
can
directly
bind
gene
loci
related
inflammation,
phosphorylation,
metabolism,
thereby
modulating
expression.
regulatory
process
inhibited
by
skatole.
At
chondrocyte
level,
conditional
medium
from
skatole-treated
balanced
anabolism
catabolism
mouse
chondrocytes
apoptosis.
IL1β-treated
chondrocytes,
suppressed
inflammation
without
affecting
apoptosis
or
anabolism.
Overall,
maintains
immune
microenvironment
homeostasis
joints
preserves
function
balancing
catabolism,
effectively
alleviating
OA.
These
findings
suggest
skatole’s
potential
DMOAD.
ACS Biomaterials Science & Engineering,
Год журнала:
2024,
Номер
10(7), С. 4411 - 4424
Опубликована: Июнь 24, 2024
Diabetic
bone
defects,
exacerbated
by
hyperglycemia-induced
inflammation
and
oxidative
stress,
present
significant
therapeutic
challenges.
This
study
introduces
a
novel
injectable
scaffold,
MgH2@PLGA/F-GM,
consisting
of
foamed
gelatin-methacryloyl
(GelMA)
magnesium
hydride
(MgH2)
microspheres
encapsulated
in
poly(lactic-co-glycolic
acid)
(PLGA).
scaffold
is
uniquely
suited
for
diabetic
conforming
to
complex
shapes
fostering
an
environment
conducive
tissue
regeneration.
As
it
degrades,
Mg(OH)2
released
dissolved
PLGA's
acidic
byproducts,
releasing
Mg2+
ions.
These
ions
are
instrumental
macrophage
phenotype
modulation,
reduction,
angiogenesis
promotion,
all
vital
healing.
Additionally,
hydrogen
(H2)
during
degradation
mitigates
stress
diminishing
reactive
oxygen
species
(ROS).
multifaceted
approach
not
only
reduces
ROS
but
also
enhances
M2
polarization
cell
migration,
culminating
improved
repair.
presents
innovative
strategy
addressing
the
complexities
defect
treatment.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Март 19, 2024
Background:
The
cartilage
tissue
lacks
blood
vessels,
which
is
composed
of
chondrocytes
and
ECM.
Due
to
this
vessel-less
structure,
it
difficult
repair
damages.
One
the
new
methods
damage
use
engineering.
In
present
study,
was
attempted
simulate
a
three-dimensional
environment
similar
natural
ECM
by
using
hydrogels
made
materials,
including
Chitosan
different
ratios
Alginate.
Material
methods:
Chitosan,
alginate
Chitosan/Alginate
were
fabricated.
Fourier
Transform
Infrared,
XRD,
swelling
ratio,
porosity
measurement
degradation
tests
applied
scaffolds
characterization.
After
that,
human
adipose
derived-mesenchymal
stem
cells
(hADMSCs)
cultured
on
then
their
viability
chondrogenic
differentiation
capacity
studied.
Safranin
O
Alcian
blue
staining,
immunofluorescence
staining
real
time
RT-PCR
used
as
analytical
for
potential
evaluation
hADMSCs
when
hydrogels.
Results:
highest
rate
detected
in
(1:0.5)
group
scaffold
biocompatibility
results
revealed
that
groups
not
significantly
with
control
group.
PCR
grown
hydrogel
higher
than
those
cell
other
groups.
Conclusion:
Taken
together,
these
suggest
could
be
promising
candidate
engineering
applications.
Frontiers in Veterinary Science,
Год журнала:
2025,
Номер
11
Опубликована: Янв. 8, 2025
Eggshell
membrane
(ESM)
is
a
rich
source
of
bioactive
compounds,
including
proteins,
peptides,
and
antioxidants,
contributing
to
its
potential
therapeutic
benefits.
These
natural
antioxidants
might
help
neutralize
reactive
oxygen
species
(ROS)
modulate
inflammatory
responses,
which
are
often
linked
with
chondrocyte
damage
in
osteoarthritis.
In
this
study,
we
investigated
the
functional
effects
ESM
proteins
on
H2O2-induced
oxidative
stress
neonatal
canine
chondrocytes.
The
isolated
chondrocytes
demonstrated
high
proliferation
rate
increased
glycosaminoglycan
(GAG)
production
during
cultivation.
addition,
expression
key
cartilage
markers,
collagen
types
II
IX,
aggrecan,
confirmed
retention
phenotype.
Under
vitro
conditions,
post-treatment
improved
viability,
indicating
that
may
have
reparative
role
mitigating
damage.
This
significant
was
validated
through
XTT
assays,
measured
cell
metabolic
activity
at
24
h,
Real-time
Cell
Analysis
(RTCA),
providing
continuous
monitoring
over
98
h.
contrast,
preventive
against
were
observed
exclusively
analysis.
By
investigating
these
aspects,
provide
insight
into
protect
from
damage,
particularly
repair
joint
health.
study
one
first
create
vital
platform
based
for
dietary
supplements
designed
prevent
or
dog
Thus,
offers
valuable
contribution
understanding
how
compounds
can
be
used
therapeutically,
bridging
gap
between
findings
practical
applications
veterinary
medicine.
