Bioactive Porous Composite Implant Guides Mesenchymal Stem Cell Differentiation and Migration to Accelerate Bone Reconstruction DOI Creative Commons
Sheng Wang, Demeng Xia,

Wenxue Dou

и другие.

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 12111 - 12127

Опубликована: Ноя. 1, 2024

Delayed healing and non-healing of bone defects pose significant challenges in clinical practice, with metal materials increasingly recognized for their significance addressing these issues. Among materials, Strontium (Sr) Zinc (Zn) have emerged as promising agents promoting repair. Building upon this insight, research evaluates the impact a porous Sr@Zn@SiO2 nanocomposite implant on regeneration, aiming to advance field The preparation composite involves various techniques such roasting, centrifugation, washing. material's composition is examined, its microstructure element distribution are analyzed using TEM elemental scanning technology. In vitro experiments entail isolation characterization BMSCs followed by safety assessments material, evaluation cell migration capabilities, relevant proliferation markers. Mechanistically, study delves into key targets associated changes osteogenic process. vivo involve establishing rat femur defect model, assessment potential Micro-CT imaging tissue section staining. Through investigations, we validate efficacy implant. analyses demonstrate that upregulate BMP-2 expression, leading activation Smad1/5/9 phosphorylation subsequent downstream genes, culminating differentiation proliferation. And closely related high expression CXCL12/CXCR4, which will also provide conditions osteogenesis. vivo, ability was confirmed rats. our research, displays prominent effect promotes promote healing. This bioactive has surgical application future.

Язык: Английский

Bioactive Porous Composite Implant Guides Mesenchymal Stem Cell Differentiation and Migration to Accelerate Bone Reconstruction DOI Creative Commons
Sheng Wang, Demeng Xia,

Wenxue Dou

и другие.

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 12111 - 12127

Опубликована: Ноя. 1, 2024

Delayed healing and non-healing of bone defects pose significant challenges in clinical practice, with metal materials increasingly recognized for their significance addressing these issues. Among materials, Strontium (Sr) Zinc (Zn) have emerged as promising agents promoting repair. Building upon this insight, research evaluates the impact a porous Sr@Zn@SiO2 nanocomposite implant on regeneration, aiming to advance field The preparation composite involves various techniques such roasting, centrifugation, washing. material's composition is examined, its microstructure element distribution are analyzed using TEM elemental scanning technology. In vitro experiments entail isolation characterization BMSCs followed by safety assessments material, evaluation cell migration capabilities, relevant proliferation markers. Mechanistically, study delves into key targets associated changes osteogenic process. vivo involve establishing rat femur defect model, assessment potential Micro-CT imaging tissue section staining. Through investigations, we validate efficacy implant. analyses demonstrate that upregulate BMP-2 expression, leading activation Smad1/5/9 phosphorylation subsequent downstream genes, culminating differentiation proliferation. And closely related high expression CXCL12/CXCR4, which will also provide conditions osteogenesis. vivo, ability was confirmed rats. our research, displays prominent effect promotes promote healing. This bioactive has surgical application future.

Язык: Английский

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