Expression and Immune Response Profiles in Nile Tilapia (Oreochromis niloticus) and European Sea Bass (Dicentrarchus labrax) During Pathogen Challenge and Infection DOI Open Access
Ahmed A. Saleh,

A. Mohamed,

Shaaban S. Elnesr

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12829 - 12829

Опубликована: Ноя. 28, 2024

Nile tilapia (Oreochromis niloticus) and European sea bass (Dicentrarchus labrax) are economically significant species in Mediterranean countries, serving essential roles the aquaculture industry due to high market demand nutritional value. They experience substantial losses from bacterial pathogens Vibrio anguillarum Streptococcus iniae, particularly at onset of summer season. The immune mechanisms involved fish infections by V. S. iniae remain poorly understood. This study investigated their impact through experiments with control anguillarum- iniae-infected groups for each species. Blood samples were collected 1, 3, 7 days post injection assess biochemical immunological parameters, including enzyme activities (AST ALT), oxidative markers (SOD, GPX, CAT, MDA), leukocyte counts. Further analyses included phagocyte activity, lysozyme IgM levels, complement C3 C4 levels. Muscle tissues sampled mRNA expression levels 18 immune-relevant genes. focus was on cytokines immune-related genes, pro-inflammatory (TNF-α, TNF-β, IL-2, IL-6, IL-8, IL-12, IFN-γ), major histocompatibility complex components (MHC-IIα MHC-IIβ), cytokine receptors (CXCL-10 CD4-L2), antimicrobial peptides (Pleurocidin β-defensin), regulatory (Thymosin β12, Leap 2, Lysozyme g), Galectins (Galectin-8 Galectin-9). β-actin used as housekeeping gene normalization. Significant species-specific responses observed N. Tilapia E. Sea Bass when infected highlighting differences biochemical, immune, profiles. Notably, Tilapia, AST significantly increased day during infection, reaching 45.00 ± 3.00 (p < 0.05), indicating late-stage acute stress or tissue damage. Conversely, exhibited a rise ALT group, peaking 33.5 3.20 suggesting liver distress systemic inflammatory response. On front, showed increases respiratory burst activity 1 both pathogens, values 0.28 0.03 0.25 0.02 robust initial activation. Finally, analysis revealed pronounced peak TNF-α infection fold change 6.120, strong response strategy. Understanding these provides critical insights enhancing disease management productivity operations.

Язык: Английский

Chronic Obstructive Pulmonary Disease and COVID-19: The Impact of Hematological Biomarkers on Disease Severity and Outcomes DOI Open Access

G Mara,

Gheorghe Nini,

Coralia Cotoraci

и другие.

Journal of Clinical Medicine, Год журнала: 2025, Номер 14(8), С. 2765 - 2765

Опубликована: Апрель 17, 2025

Background/Objectives: Chronic obstructive pulmonary disease (COPD) patients are at heightened risk of severe COVID-19 due to underlying respiratory impairment, systemic inflammation, and immune dysregulation. This review explores the hematological changes that occur in COPD with their implications for progression, prognosis, clinical management. Methods: We conducted a comprehensive analysis recent peer-reviewed studies from medical databases including Clarivate Analytics, PubMed, Google Scholar. Results: Hematological alterations, such as lymphopenia, elevated neutrophil-to-lymphocyte ratio (NLR), increased D-dimer fibrinogen levels, inflammatory anemia, erythrocyte dysfunction, commonly observed COVID-19. These linked suppression, hyperinflammation, oxidative stress, thromboembolic complications. Conclusions: biomarkers valuable tools early assessments guiding treatment strategies this high-risk population. The regular monitoring D-dimer, fibrinogen, NLR is advisable. Prophylactic anticoagulation immunomodulatory therapies, corticosteroids IL-6 IL-1 inhibitors, may improve outcomes. Further needed validate personalized approaches explore antioxidant-based interventions.

Язык: Английский

Процитировано

0
Expression and Immune Response Profiles in Nile Tilapia (Oreochromis niloticus) and European Sea Bass (Dicentrarchus labrax) During Pathogen Challenge and Infection DOI Open Access
Ahmed A. Saleh,

A. Mohamed,

Shaaban S. Elnesr

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12829 - 12829

Опубликована: Ноя. 28, 2024

Nile tilapia (Oreochromis niloticus) and European sea bass (Dicentrarchus labrax) are economically significant species in Mediterranean countries, serving essential roles the aquaculture industry due to high market demand nutritional value. They experience substantial losses from bacterial pathogens Vibrio anguillarum Streptococcus iniae, particularly at onset of summer season. The immune mechanisms involved fish infections by V. S. iniae remain poorly understood. This study investigated their impact through experiments with control anguillarum- iniae-infected groups for each species. Blood samples were collected 1, 3, 7 days post injection assess biochemical immunological parameters, including enzyme activities (AST ALT), oxidative markers (SOD, GPX, CAT, MDA), leukocyte counts. Further analyses included phagocyte activity, lysozyme IgM levels, complement C3 C4 levels. Muscle tissues sampled mRNA expression levels 18 immune-relevant genes. focus was on cytokines immune-related genes, pro-inflammatory (TNF-α, TNF-β, IL-2, IL-6, IL-8, IL-12, IFN-γ), major histocompatibility complex components (MHC-IIα MHC-IIβ), cytokine receptors (CXCL-10 CD4-L2), antimicrobial peptides (Pleurocidin β-defensin), regulatory (Thymosin β12, Leap 2, Lysozyme g), Galectins (Galectin-8 Galectin-9). β-actin used as housekeeping gene normalization. Significant species-specific responses observed N. Tilapia E. Sea Bass when infected highlighting differences biochemical, immune, profiles. Notably, Tilapia, AST significantly increased day during infection, reaching 45.00 ± 3.00 (p < 0.05), indicating late-stage acute stress or tissue damage. Conversely, exhibited a rise ALT group, peaking 33.5 3.20 suggesting liver distress systemic inflammatory response. On front, showed increases respiratory burst activity 1 both pathogens, values 0.28 0.03 0.25 0.02 robust initial activation. Finally, analysis revealed pronounced peak TNF-α infection fold change 6.120, strong response strategy. Understanding these provides critical insights enhancing disease management productivity operations.

Язык: Английский

Процитировано

0