Biomolecules,
Год журнала:
2024,
Номер
14(5), С. 554 - 554
Опубликована: Май 4, 2024
Osteoporosis
(OP),
a
prevalent
skeletal
disorder
characterized
by
compromised
bone
strength
and
increased
susceptibility
to
fractures,
poses
significant
public
health
concern.
This
review
aims
provide
comprehensive
analysis
of
the
current
state
research
in
field,
focusing
on
application
proteomic
techniques
elucidate
diagnostic
markers
therapeutic
targets
for
OP.
The
integration
cutting-edge
technologies
has
enabled
identification
quantification
proteins
associated
with
metabolism,
leading
deeper
understanding
molecular
mechanisms
underlying
In
this
review,
we
systematically
examine
recent
advancements
studies
related
OP,
emphasizing
potential
biomarkers
OP
diagnosis
discovery
novel
targets.
Additionally,
discuss
challenges
future
directions
highlighting
impact
transforming
landscape
treatment.
Journal of Separation Science,
Год журнала:
2025,
Номер
48(2)
Опубликована: Фев. 1, 2025
ABSTRACT
Metabolomics,
a
powerful
discipline
within
systems
biology,
aims
at
comprehensive
profiling
of
small
molecules
in
biological
samples.
The
challenges
sample
complexity
are
addressed
through
innovative
preparation
methods,
including
solid‐phase
extraction
and
microextraction
techniques,
enhancing
the
detection
quantification
low‐abundance
metabolites.
Advances
chromatographic
separation,
particularly
liquid
chromatography
(LC)
gas
(GC),
coupled
with
high‐resolution
(HR)
mass
spectrometry
(MS),
have
significantly
improved
sensitivity,
selectivity,
throughput
metabolomic
studies.
Cutting‐edge
such
as
ion‐mobility
(IM‐MS)
tandem
MS
(MS/MS),
further
expand
capacity
for
metabolite
profiling.
These
advanced
analytical
platforms
each
offer
unique
advantages
metabolomics,
continued
technological
improvements
driving
deeper
insights
into
metabolic
pathways
biomarker
discovery.
By
providing
detailed
overview
current
trends
this
review
to
valuable
future
metabolomics
human
health
research
its
translational
potential
clinical
settings.
Toward
end,
also
highlights
biomedical
applications
emphasizing
role
discovery,
disease
diagnostics,
personalized
medicine,
drug
development.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(13), С. 7347 - 7347
Опубликована: Июль 4, 2024
Chronic
obstructive
pulmonary
disease
(COPD)
plays
a
significant
role
in
global
morbidity
and
mortality
rates,
typified
by
progressive
airflow
restriction
lingering
respiratory
symptoms.
Recent
explorations
molecular
biology
have
illuminated
the
complex
mechanisms
underpinning
COPD
pathogenesis,
providing
critical
insights
into
progression,
exacerbations,
potential
therapeutic
interventions.
This
review
delivers
thorough
examination
of
latest
progress
research
related
to
COPD,
involving
fundamental
pathways,
biomarkers,
targets,
cutting-edge
technologies.
Key
areas
focus
include
roles
inflammation,
oxidative
stress,
protease-antiprotease
imbalances,
alongside
genetic
epigenetic
factors
contributing
susceptibility
heterogeneity.
Additionally,
advancements
omics
technologies-such
as
genomics,
transcriptomics,
proteomics,
metabolomics-offer
new
avenues
for
comprehensive
profiling,
aiding
discovery
novel
biomarkers
targets.
Comprehending
foundation
carries
substantial
creation
tailored
treatment
strategies
enhancement
patient
outcomes.
By
integrating
clinical
practice,
there
is
promising
pathway
towards
personalized
medicine
approaches
that
can
improve
diagnosis,
treatment,
overall
management
ultimately
reducing
its
burden.
Biomolecules,
Год журнала:
2025,
Номер
15(1), С. 130 - 130
Опубликована: Янв. 15, 2025
Proteomics
accelerates
diagnosis
and
research
of
muscular
diseases
by
enabling
the
robust
analysis
proteins
relevant
for
manifestation
neuromuscular
in
following
aspects:
(i)
evaluation
effect
genetic
variants
on
corresponding
protein,
(ii)
prediction
underlying
defect
based
proteomic
signature
muscle
biopsies,
(iii)
pathophysiologies
different
entities
diseases,
key
definition
new
intervention
concepts,
(iv)
patient
stratification
according
to
biochemical
fingerprints
as
well
(v)
monitoring
success
therapeutic
interventions.
This
review
presents—also
through
exemplary
case
studies—the
various
advantages
mass
proteomics
investigation
discusses
technical
limitations,
provides
an
outlook
possible
future
application
concepts.
Hence,
is
excellent
large-scale
analytical
tool
diagnostic
workup
(hereditary)
warrants
systematic
profiling
pathophysiological
processes.
The
steady
development
may
allow
overcome
existing
limitations
including
a
quenched
dynamic
range
quantification
protein
isoforms.
Future
directions
include
targeted
settings
using
not
only
biopsies
but
also
liquid
address
need
minimally
invasive
procedures.
Non-communicable
diseases
(NCDs)
such
as
cardiovascular
diseases,
chronic
respiratory
cancers,
diabetes,
and
mental
health
disorders
pose
a
significant
global
challenge,
accounting
for
the
majority
of
fatalities
disability-adjusted
life
years
worldwide.
These
arise
from
complex
interactions
between
genetic,
behavioral,
environmental
factors,
necessitating
thorough
understanding
these
dynamics
to
identify
effective
diagnostic
strategies
interventions.
Although
recent
advances
in
multi-omics
technologies
have
greatly
enhanced
our
ability
explore
interactions,
several
challenges
remain.
include
inherent
complexity
heterogeneity
multi-omic
datasets,
limitations
analytical
approaches,
severe
underrepresentation
non-European
genetic
ancestries
most
omics
which
restricts
generalizability
findings
exacerbates
disparities.
This
scoping
review
evaluates
landscape
data
related
NCDs
2000
2024,
focusing
on
advancements
integration,
translational
applications,
equity
considerations.
We
highlight
need
standardized
protocols,
harmonized
data-sharing
policies,
advanced
approaches
artificial
intelligence/machine
learning
integrate
study
gene-environment
interactions.
also
opportunities
translating
insights
(GxE)
research
into
precision
medicine
strategies.
underscore
potential
advancing
enhancing
patient
outcomes
across
diverse
underserved
populations,
emphasizing
fairness-centered
strategic
investments
build
local
capacities
underrepresented
populations
regions.
Expert Review of Proteomics,
Год журнала:
2024,
Номер
21(4), С. 149 - 168
Опубликована: Апрель 2, 2024
Introduction
Saliva
has
gained
increasing
attention
in
the
quest
for
disease
biomarkers.
Because
it
is
a
biological
fluid
that
can
be
collected
an
easy,
painless,
and
safe
way,
been
increasingly
studied
identification
of
oral
cancer
This
particularly
important
because
often
diagnosed
at
late
stages
with
poor
prognosis.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 9880 - 9880
Опубликована: Сен. 12, 2024
Mass
spectrometry
(MS)
has
revolutionized
clinical
chemistry,
offering
unparalleled
capabilities
for
biomolecule
analysis.
This
review
explores
the
growing
significance
of
mass
(MS),
particularly
when
coupled
with
liquid
chromatography
(LC),
in
identifying
disease
biomarkers
and
quantifying
biomolecules
diagnostic
prognostic
purposes.
The
unique
advantages
MS
accurately
diverse
molecules
have
positioned
it
as
a
cornerstone
personalized-medicine
advancement.
MS-based
technologies
transformed
precision
medicine,
enabling
comprehensive
understanding
mechanisms
patient-specific
treatment
responses.
LC-MS
shown
exceptional
utility
analyzing
complex
biological
matrices,
while
high-resolution
expanded
analytical
capabilities,
allowing
detection
low-abundance
elucidation
pathways.
integration
other
techniques,
such
ion
mobility
spectrometry,
opened
new
avenues
biomarker
discovery
validation.
As
we
progress
toward
will
be
crucial
addressing
challenges
individualized
patient
care,
driving
innovations
diagnosis,
prognosis,
strategies.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(23), С. 16931 - 16931
Опубликована: Ноя. 29, 2023
Gastric
cancer
(GC)
is
a
highly
malignant
disease
affecting
humans
worldwide
and
has
poor
prognosis.
Most
GC
cases
are
detected
at
advanced
stages
due
to
the
lacking
early
detectable
symptoms.
Therefore,
there
great
interest
in
improving
diagnosis
by
implementing
targeted
prevention
strategies.
Markers
necessary
for
detection
guide
clinicians
best
personalized
treatment.
The
current
semi-invasive
endoscopic
methods
detect
invasive,
costly,
time-consuming.
Recent
advances
proteomics
technologies
have
enabled
screening
of
many
samples
novel
biomarkers
disease-related
signature
signaling
networks.
These
include
circulating
proteins
from
different
fluids
(e.g.,
plasma,
serum,
urine,
saliva)
extracellular
vesicles.
We
review
relevant
published
studies
on
protein
detail
their
application
as
potential
diagnosis.
Identifying
sensitive
specific
diagnostic
markers
may
improve
patient
survival
rates
contribute
advancing
precision/personalized
medicine.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 22, 2024
Clinical
biomarker
development
has
been
stymied
by
inaccurate
protein
quantification
from
mass
spectrometry
(MS)
discovery
data
and
a
prolonged
validation
process.
To
mitigate
these
issues,
we
created
the
Targeted
Extraction
Assessment
of
Quantification
(TEAQ)
software
package.
This
innovative
tool
uses
cohort
analysis
to
select
precursors,
peptides,
proteins
that
adhere
established
targeted
assay
criteria.
TEAQ
was
applied
Data-Independent
Acquisition
MS
plasma
samples
acquired
on
an
Orbitrap™
Astral™
MS.
Identified
precursors
were
evaluated
for
linearity,
specificity,
repeatability,
reproducibility,
intra-protein
correlation
11-point
loading
curves
under
three
throughputs,
develop
resource
clinical-grade
assays.
From
clinical
individuals
with
inflammatory
bowel
disease
(n=492),
successfully
identified
1116
signature
peptides
327
quantifiable
1180
proteins.
Embedding
stringent
selection
criteria
adaptable
into
will
streamline
transition
studies.
