Advances in sarcopenia: mechanisms, therapeutic targets, and intervention strategies

Archives of Pharmacal Research, Год журнала: 2024, Номер 47(4), С. 301 - 324

Опубликована: Апрель 1, 2024

Язык: Английский

---

Electroporation Delivery of Cas9 sgRNA Ribonucleoprotein-Mediated Genome Editing in Sheep IVF Zygotes

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9145 - 9145

Опубликована: Авг. 23, 2024

The utilization of electroporation for delivering CRISPR/Cas9 system components has enabled efficient gene editing in mammalian zygotes, facilitating the development genome-edited animals. In this study, our research focused on targeting

Язык: Английский

---

Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR

Genes, Год журнала: 2023, Номер 14(6), С. 1216 - 1216

Опубликована: Июнь 2, 2023

Hu sheep, an indigenous breed in China known for its high fecundity, are being studied to improve their growth and carcass traits. MSTN is a negative regulator of muscle development, inactivation results muscularity. The C-CRISPR system, utilizing multiple neighboring sgRNAs targeting key exon, has been successfully used generate genes complete knockout (KO) monkeys mice one step. In this study, the system was MSTN-edited sheep; 70 embryos injected with Cas9 mRNA four exon 3 sheep were transferred 13 recipients. Out 10 lambs born from five recipients after full-term pregnancies, nine had KO various mutations. No off-target effects found. These MSTN-KO showed double-muscled (DM) phenotype, characterized by higher body weight at 4 months old, prominent muscular protrusion, clearly visible intermuscular groves, hypertrophy. molecular analysis indicated enhanced AKT suppressed ERK1/2 signaling gluteus edited sheep. conclusion, DM phenotype efficiently specifically generated using C-CRISPR, method promising tool farm animal breeding.

Язык: Английский

---

Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats

Agriculture, Год журнала: 2024, Номер 14(3), С. 487 - 487

Опубликована: Март 18, 2024

Due to recent innovations in gene editing technology, great progress has been made livestock breeding, with researchers rearing gene-edited pigs, cattle, sheep, and other livestock. Gene-editing technology involves knocking in, out, deleting, inhibiting, activating, or replacing specific bases of DNA RNA sequences at the genome level for accurate modification, such processes can edit genes a fixed point without needing templates. In years, although clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system-mediated gene-editing widely used research into genetic breeding animals, system’s efficiency inserting foreign is not high enough, there are certain off-target effects; thus, it appropriate use large as cashmere goats. this study, development status, associated challenges, application prospects, future prospects CRISPR/Cas9-mediated precision were reviewed provide theoretical reference function analysis, improvement, that account characteristics local economies.

Язык: Английский

---

When Less Is More: Targeting the Myostatin Gene in Livestock for Augmenting Meat Production

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(10), С. 4216 - 4227

Опубликована: Март 2, 2023

How to increase meat production is one of the main questions in animal breeding. Selection for improved body weight has been made and, due recent genomic advances, naturally occurring variants that are responsible controlling economically relevant phenotypes have revealed. The myostatin (MSTN) gene, a superstar gene breeding, was discovered as negative controller muscle mass. In some livestock species, natural mutations MSTN could generate agriculturally desirable double-muscling phenotype. However, other species or breeds lack these variants. Genetic modification, particularly editing, offers an unprecedented opportunity induce mimic genomes. To date, various MSTN-edited generated using different modification tools. These gene-edited models higher growth rates and increased mass, suggesting high potential utilizing editing Additionally, post-editing investigations most support favorable influence targeting on quantity quality. this Review, we provide collective discussion further encourage its utilization opportunities. It expected that, shortly, will be commercialized, tables ordinary customers.

Язык: Английский

---

Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep

BMC Microbiology, Год журнала: 2022, Номер 22(1)

Опубликована: Ноя. 11, 2022

Abstract Background Myostatin ( MSTN ) negatively regulates the muscle growth in animals and deficient sheep have been widely reported previously. The goal of this study was to explore how inactivation influences their gut microbiota composition potential functions. Results We compared slaughter parameters meat quality 3 -edited male wild-type sheep, analyzed microbiome (8 female 8 sheep) through metagenomic sequencing. results showed that body weight, carcass weight eye area were significantly higher, but there no significant differences indexes. At microbial level, alpha diversity higher P < 0.05), different by PCoA analysis sheep. abundance Firmicutes increased Bacteroidota decreased genus Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium Lactobacillus 0.05). Further functional found carotenoid biosynthesis peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, adipocytokines pathways Moreover, carbohydrate-active enzymes (CAZymes) certified GH13_39, GH4, GH137, GH71 PL17 upregulated, GT41 CBM20 downregulated Conclusions Our suggested remarkably influenced function hindgut communities promoted performance without affecting quality.

Язык: Английский

---

New Genomic Techniques (NGT) in animals and their agri/food/feed products

EFSA Supporting Publications, Год журнала: 2023, Номер 20(9)

Опубликована: Сен. 1, 2023

This report presents a review of the commercial and pre-commercial stage applications new genomic technologies (NGT) applied to farm animals their agri/food/feed products.Additionally, literature was performed compile comprehensive listing peerreviewed research development gene edited for food agricultural applications.A total 195 publications resulting in live were compiled.To date, several developed or ongoing have been authorized commerce, judged be "non-GMO" hence conventional, at least one country including knockout tiger pufferfish red sea bream Japan; tilapia, cattle, pigs horses Argentina; cattle tilapia Brazil; two gene-edited granted enforcement discretion United States meaning products can enter supply.One application, targeted exon deletion porcine respiratory reproductive syndrome virus resistance is formally precommercial stage.There are proof-of-concept multiple species testing targets traits interest.The most common trait category meat fibre yield (31%), followed by reproduction (24%), biotic stress (18%), (7%), colour (6%), production hypoallergenic (5%), product quality (4%), abiotic (1%), other (4%).The majority these SDN-1 using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 introduce small insertions deletions inactivate gene.The large number focused on due part interest both single-sex offspring numerous industries (e.g.females case egg production), infertility coupled with germline complementation chimeras (where germline-competent donor cells used replace an otherwise sterile host different genetic background) finfish, chickens, goats, pigs.

Язык: Английский

---

5个SNP突变位点对乌珠穆沁羊不同时期体重的影响

Scientia Sinica Vitae, Год журнала: 2024, Номер unknown

Опубликована: Март 1, 2024

绵羊的体重大小能直接反映羊只的生长速度、肥育速度, 也是衡量肉羊生长性能的重要指标. 本研究以乌珠穆沁羊为研究对象. 挑选出100个与绵羊体型相关的SNP位点, 同时采集252只乌珠穆沁羊的血液和不同时期体重数据, 使用MassARRAY基因分型技术对这些绵羊在选定的SNP位点进行基因分型, 并将体重数据与基因分型结果进行关联分析. 结果表明, 本研究共验证出5个突变位点与乌珠穆沁羊不同时期体重显著相关. 进一步对3个错义突变位点进行蛋白结构预测分析发现, 3个突变位点CDS序列改变导致其蛋白质三级结构发生不同程度改变, 进而导致个体体重差异. 本研究旨在挖掘与乌珠穆沁羊生长性状相关的特征位点, 为乌珠穆沁羊今后的精准选育提供参考, 以期推动乌珠穆沁羊产业的发展.

---

Genetic Variations of MSTN and Callipyge in Tibetan Sheep: Implications for Early Growth Traits

Genes, Год журнала: 2024, Номер 15(7), С. 921 - 921

Опубликована: Июль 15, 2024

Tibetan sheep are vital to the ecosystem and livelihood of Plateau; however, traditional breeding methods limit their production growth. Modern molecular techniques required improve these traits. This study identified a single nucleotide polymorphism (SNP) in myostatin (MSTN) Callipyge sheep. The findings indicated notable associations between MSTN genotypes growth traits including birth weight (BW), body length (BL), chest width (ChW), circumference (ChC), as well particularly strong association with cannon (CaC) at 2 months age. Conversely, polymorphisms did not have significant impact on Moreover, analyses revealed sex BW or hip (HW) age ChW, ChC, CaC 4 Furthermore, study’s results suggested that genotype GA was associated effect BW, while (CC) showed These SNP could potentially serve marker for early

Язык: Английский

---

Opportunities for CRISPR-Cas9 application in farm animal genetic improvement

Molecular Biology Reports, Год журнала: 2024, Номер 51(1)

Опубликована: Окт. 30, 2024

Язык: Английский

---