Aquaculture Reports, Год журнала: 2024, Номер 40, С. 102586 - 102586
Опубликована: Дек. 28, 2024
Язык: Английский
Cells, Год журнала: 2023, Номер 12(14), С. 1818 - 1818
Опубликована: Июль 10, 2023
The CRISPR/Cas9 system is widely used for genome editing in livestock production, although off-target effects can occur. It the main method to produce genome-edited goats by somatic cell nuclear transfer (SCNT) of CRISPR/Cas9-mediated primary goat fetal fibroblast cells (GFFs). Improving double-strand break (DSB) efficiency Cas9 would improve homologous repair (HR) efficiency. low HR remains a major hurdle precise editing, increasing work required screen clones. In this study, we modified several essential parameters that affect knock-in GFF cloning system, including establishing high-efficiency transfection via nucleofection and optimizing homology arm (HA) length during HR. Here, specifically inserted recombinant human butyrylcholinesterase gene (rhBChE) into growth factor (FGF)-5 locus through thereby achieving simultaneous rhBChE insertion FGF5 knock-out. First, study introduced Cas9, knock-out small guide RNA, donors GFFs electroporation obtained positive clones without effects. Then, demonstrated expression verified its function. Finally, rhBChE-overexpression goat.
Язык: Английский
Процитировано
3
Frontiers in Veterinary Science, Год журнала: 2024, Номер 11
Опубликована: Май 2, 2024
Sheep body size can directly reflect the growth rates and fattening of sheep is also an important index for measuring performance meat sheep. In this study, high-resolution resequencing data from four breeds (Dorper sheep, Suffolk Ouessant Shetland sheep) were analyzed. The nonsynonymous single nucleotide polymorphisms three candidate genes ( KIAA1217 , SNTA1 LTBP1 ) genotyped in 642 healthy Ujumqin using MALDI-TOFMS genotyping results associated with traits. showed that different genotypes g.24429511T>C locus had significant effects on chest circumferences g.62222626C>A depths, shoulder widths rump This study these two sites be used marker-assisted selection, which will beneficial future precision molecular breeding.
Язык: Английский
Процитировано
0
Gene, Год журнала: 2024, Номер unknown, С. 149044 - 149044
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Дек. 9, 2024
ABSTRACT The guinea pig ( Cavia porcellus ), a species native to Peru, is valued for its meat production, where muscle development essential productive efficiency. new Kuri breed, obtained through selective breeding and genetic selection, has shown phenotype with more developed musculature compared pigs. In this study, we conducted comparative transcriptomic analysis between Native breed pig, complemented by histological of the semitendinosus investigate underlying mechanisms responsible differences in morphology. Histological revealed significant increase fiber area At molecular level, key mutations were identified MSTN gene, including variants 3’ UTR region frameshift mutation, which alter gene’s inhibitory function on growth. Additionally, observed expression pathways related degradation, energy metabolism, angiogenesis, explain greater hypertrophy breed. These findings provide first understanding suggest candidate genes improving quality programs AUTHOR SUMMARY Peru production. selection other breeders. our showed native. Furthermore, it time that identify impact finding will allow us plan improvement strategies promote hypertrophy, paving way future research potential
Язык: Английский
Процитировано
0
Aquaculture Reports, Год журнала: 2024, Номер 40, С. 102586 - 102586
Опубликована: Дек. 28, 2024
Язык: Английский
Процитировано
0