Molecular Syndromology,
Год журнала:
2023,
Номер
15(1), С. 37 - 42
Опубликована: Сен. 13, 2023
<b><i>Objective:</i></b>
The
study
aimed
to
explore
the
genotype
and
allele
distributions
of
dopamine
D2-like
receptor
(<i>DRD2</i>)
gene
-141C
C957T
polymorphisms
in
Chinese
Han
population
with
dyslipidemia,
as
well
their
association
serum
lipid
levels.
<b><i>Methods:</i></b>
One
hundred
fifty
patients
dyslipidemia
150
healthy
people
were
recruited
case
control
groups,
respectively.
Serum
total
cholesterol
(TC),
triglyceride
(TG),
high-density
lipoprotein
(HDL-C),
low-density
levels
detected.
target
sequence
<i>DRD2</i>
was
amplified
by
polymerase
chain
reaction
genotyped
via
Sanger
sequencing.
<b><i>Results:</i></b>
In
(rs6277),
three
genotypes
CC,
CT,
TT
detected
frequencies
92.67%,
6.67%,
0.67%
cases,
83.33%,
14.67%,
2.00%
controls,
CT
T
significantly
low
group
relative
group.
After
adjusting
other
clinical
indicators,
polymorphism
(hazard
ratio
=
0.401,
95%
confidence
interval
0.181–0.890,
<i>p</i>
<
0.05)
still
related
a
reduced
risk
dyslipidemia.
carriers
had
lowest
values
TC,
TG,
LDL,
highest
HDL-C.
<b><i>Conclusion:</i></b>
an
independent
influencing
factor
associated
susceptibility
decreased
odds
Journal of Systematics and Evolution,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 9, 2025
Abstract
Viola
philippica
develops
chasmogamous
(CH)
and
cleistogamous
(CL)
flowers
under
different
photoperiods,
while
cornuta
only
forms
CH
flowers.
Gene
expression
variations
driven
by
photoperiods
that
contribute
to
the
CH–CL
transition
remain
largely
unknown.
Here,
we
performed
comparative
transcriptomics
between
V
.
during
flower
development
revealed
differentially
expressed
genes
(DEGs)
CL
Compared
in
16‐h
daylight
conditions,
DEGs,
including
auxin
response
factor
(ARF)
floral
MADS‐box
genes,
were
identified
12‐h
conditions;
however,
their
homologous
equivalently
across
both
photoperiods.
Genes
modules
most
significantly
correlated
with
types
DEGs
,
but
they
not
altered
either
photoperiod.
Moreover,
related
fatty
acid,
flavonoid,
anthocyanin
biosynthesis
upregulated
flowers,
whereas
defense
Furthermore,
co‐expressed
B‐class
gene
APETALA3
included
ARFs
CRABS
CLAW
BIGPETALp
TCP14
UGT87A
whose
are
involved
nectary
organ
development,
identity,
size,
coloration.
Thus,
putative
coordination
of
signaling
pathways,
identity
determination
growth,
acid
flavonol
photoperiod
might
regulate
thus
providing
new
insights
into
evolution
dimorphic
Chloroplast
genomes
for
3
Bidens
plants
endemic
to
China
(Bidens
bipinnata
Linn.,
pilosa
and
alba
var.
radiata)
have
been
sequenced,
assembled
annotated
in
this
study
distinguish
their
molecular
characterization
phylogenetic
relationships.
The
chloroplast
are
typical
quadripartite
structure
with
two
inverted
repeat
regions
separating
a
large
single
copy
region
small
region,
ranged
from
151,599
154,478
bp
length.
Similar
number
of
SSRs
long
repeats
were
found
Bidens,
wherein
mononucleotide
(A/T),
forward
palindromic
the
most
abundance.
Gene
loss
clpP
psbD,
IR
expansion
contraction
detected
these
plants.
It
seems
that
ndhE,
ndhF,
ndhG,
rpl32
under
positive
selection
while
majority
genes
purifying
selection.
Phylogenetic
analysis
revealed
clustered
together
further
formed
molophyletic
clade
other
species,
indicating
might
be
radiation
adaptive
changing
environment
world-widely.
Moreover,
mutation
hotspot
silico
PCR
indicated
inter-genic
ndhD-ccsA,
ndhI-ndhG,
ndhF-rpl32,
trnL_UAG-rpl32,
ndhE-psaC,
matK-rps16,
rps2-atpI,
cemA-petA,
petN-psbM
candidate
markers
identification
This
may
provide
useful
information
genetic
diversity
species.
Scientia Horticulturae,
Год журнала:
2024,
Номер
327, С. 112833 - 112833
Опубликована: Янв. 12, 2024
The
chloroplast
genome
plays
an
important
role
in
exploring
the
origin
and
evolution
of
grapes.
In
this
study,
genomes
eight
grape
germplasms
were
analyzed.
results
show
that
sequence
arrangement
was
highly
conserved
has
a
typical
annular
quadripartite
structure.
At
same
time,
GC
content,
gene
sequence,
repeat
type
codon
bias
pattern
are
conserved.
Whereas,
19
mutation
hotspot
regions
identified
by
comparative
analysis,
including
9
protein-coding
genes
(atpF,
rpoC2,
rps18,
psbC,
atpB,
rbcL,
rps20,
ycf1
ycf15)
10
non-coding
(trnK
–
rps16,
rps16
trnQ,
trnE
trnT,
psbZ
-
trnG,
ndhC
trnV,
accD
psaI,
ycf2
trnL,
ndhF
rpl32,
ccsA
ndhD,
trnL
ycf2).
Most
these
variable
involved
physiological
process
photosynthesis.
Further
examination
photosynthetic
fluorescence
showed
obvious
difference
among
those
species
under
high-temperature
conditions.
addition,
maximum
likelihood
(ML)
evolutionary
trees
depend
on
CDS
sequences
suggest
heat
tolerance
may
be
related
to
phylogeny
grapevine.
future,
SSR
loci
also
comparison,
used
for
DNA
molecular
marker
taxonomic
studies
contribute
stress
resistance
breeding
Viruses,
Год журнала:
2025,
Номер
17(1), С. 114 - 114
Опубликована: Янв. 15, 2025
Viola
philippica,
a
medicinal
herbaceous
plant
documented
in
the
Chinese
Pharmacopoeia,
is
promising
candidate
for
research
into
plant-derived
pharmaceuticals.
However,
study
of
newly
emerging
viruses
that
threaten
cultivation
V.
philippica
remains
limited.
In
this
study,
plants
exhibiting
symptoms
such
as
leaf
yellowing,
mottled
leaves,
and
vein
chlorosis
were
collected
subjected
to
RNA
sequencing
identify
potential
viral
pathogens.
A
novel
polerovirus,
named
Philippica
Polerovirus
(VPPV),
was
identified
philippica.
VPPV
possesses
linear,
positive-sense,
single-stranded
genome
consisting
5535
nucleotides
(nt)
encodes
seven
highly
overlapping
open
reading
frames
(ORFs).
Two
recombination
events
within
ORF2,
ORF3a,
ORF3,
providing
insights
genetic
diversity
evolution
history
polerovirus.
An
infectious
cDNA
clone
successfully
constructed
shown
infect
Nicotiana
benthamiana.
Using
PVX-based
heterologous
expression
system,
P0
protein
trigger
systemic
hypersensitive
response
(HR)-like
reaction
N.
benthamiana,
indicating
functions
main
pathogenicity
determinant.
These
findings
contributed
detection
understanding
pathogenic
mechanisms
control
strategies
Molecules,
Год журнала:
2025,
Номер
30(9), С. 1922 - 1922
Опубликована: Апрель 25, 2025
Viola
yedoensis
Makino
(V.
yedoensis),
a
perennial
herb
in
the
Violaceae
family,
is
recognized
for
its
violet
flowers
and
has
longstanding
role
ethnomedicine
treating
various
inflammatory
diseases,
such
as
boils,
furuncles,
carbuncles,
both
acute
chronic
hepatitis,
among
others.
A
comprehensive
literature
review
was
conducted
utilizing
resources
including
Chinese
Pharmacopoeia,
Flora
of
China,
Web
Science,
PubMed,
Baidu
Scholar,
Google
China
National
Knowledge
Infrastructure
(CNKI).
This
paper
serves
inaugural
latest
findings
regarding
botany,
traditional
applications,
phytochemistry,
pharmacological
properties,
quality
control,
prospective
uses
V.
yedoensis.
The
objective
to
provide
robust
foundation
future
research
suggest
novel
avenues
exploring
potential
applications.
To
date,
162
chemical
constituents
have
been
isolated
from
yedoensis,
with
flavonoids
coumarins
identified
particularly
abundant.
These
compounds
exhibit
promising
activities,
anti-inflammatory,
anti-pyretic,
anti-viral,
anti-tumor,
anti-lung
injury,
anti-liver
anti-bacterial,
anti-coagulant,
anti-complement,
anti-oxidant
properties.
Despite
considerable
advancements
fundamental
on
further
investigations
are
required
elucidate
underlying
mechanisms
action
discover
additional
uncharacterized
compounds.
underscores
plant’s
significant
development
potential,
highlighting
necessity
more
in-depth
exploration.
Frontiers in Genetics,
Год журнала:
2024,
Номер
15
Опубликована: Сен. 19, 2024
Polygonati
Rhizoma
and
odorati
Rhizoma,
known
as
“Huangjing”
“Yuzhu”
in
China,
are
medicinal
Polygonatum
species
resources
with
top-grade
medical
edible
properties.
The
chloroplast
(cp)
genome
has
been
used
to
study
diversity,
evolution,
breeding
of
for
applications
genetic
engineering.
Codon
usage
bias
(CUB),
a
common
complex
natural
phenomenon,
is
essential
studies
codon
optimization
exogenous
genes,
engineering,
molecular
evolution.
However,
the
CUB
genomes
not
systematically
studied.
In
our
study,
detailed
analysis
was
performed
genomes.
We
investigated
204
plastid
protein-coding
genes
(PCGs)
4
using
CodonW
CUSP
online
software.
Through
index,
we
found
that
had
weak
bias.
addition,
results
also
showed
high
preference
AT
bases
genomes,
use
AT-ending
codons
observed
these
neutrality
plot,
ENC
PR2-Bias
correspondence
compared
mutation
pressure,
selection
most
important
factor
CUB.
Based
on
comparative
high-frequency
expression
codons,
determined
10-11
optimal
investigative
species.
Furthermore,
result
RSCU-based
cluster
relationship
between
different
could
be
well
reflected.
This
provided
an
understanding
evolution
