Integrated Analysis of Transcriptome Profiles and lncRNA–miRNA–mRNA Competing Endogenous RNA Regulatory Network to Identify Biological Functional Effects of Genes and Pathways Associated with Johne’s Disease in Dairy Cattle DOI Creative Commons
Farzad Ghafouri,

Vahid Dehghanian Reyhan,

Mostafa Sadeghi

и другие.

Non-Coding RNA, Год журнала: 2024, Номер 10(4), С. 38 - 38

Опубликована: Июнь 28, 2024

Paratuberculosis or Johne’s disease (JD), a chronic granulomatous gastroenteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), causes huge economic losses and reduces animal welfare in dairy cattle herds worldwide. At present, molecular mechanisms biological functions involved immune responses to MAP infection of are not clearly understood. Our purpose was integrate transcriptomic profiles competing endogenous RNA (ceRNA) network analyses identify key messenger RNAs (mRNAs) regulatory regulation peripheral blood mononuclear cells (PBMCs) for cattle. In total, 28 lncRNAs, 42 miRNAs, 370 mRNAs were identified integrating gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses. this regard, we 21 hub genes (CCL20, CCL5, CD40, CSF2, CXCL8, EIF2AK2, FOS, IL10, IL17A, IL1A, IL1B, IRF1, MX2, NFKB1, NFKBIA, PTGS2, SOCS3, TLR4, TNF, TNFAIP3, VCAM1) infection. Furthermore, eight candidate subnets with 29 237 detected through clustering analyses, whereas GO analysis revealed 510, 22, 11 significantly enriched terms related process, function, cellular component categories, respectively. The main metabolic-signaling pathways that included the system defense response, response cytokine, leukocyte migration, T cell activation, bacterium, NOD-like receptor, B NF-kappa B, IL-17, receptor signaling pathways. Contributions transcriptome from MAP-positive MAP-negative sample groups plus ceRNA underlying phenotypic differences intensity pathogenicity JD provided novel insights into associated

Язык: Английский

MicroRNAs modulate immunological and inflammatory responses in Holstein cattle naturally infected with Mycobacterium avium subsp. paratuberculosis DOI Creative Commons
Gerard Badia-Bringué, María Canive, Cristina Blanco‐Vázquez

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Янв. 2, 2024

MicroRNAs (miRNAs) regulate the post-transcriptional expression of genes by binding to their target mRNAs. In this study, whole miRNA sequencing was used compare miRNAs in ileocecal valve (ICV) and peripheral blood (PB) samples cows with focal or diffuse paratuberculosis (PTB)-associated lesions gut tissues versus (vs) control without lesions. Among eight differentially expressed PB from vs controls, three (miR-19a, miR-144, miR32) were also down-regulated ICV samples, we identified a total 4, 5, 18 lesions, respectively. The differential five microRNAs miR-2425-3p, miR-139, miR-101) confirmed RT-qPCR. Next, mRNA prediction performed for each miRNA. A functional analysis using predicted gene targets revealed significant enrichment RNA polymerase MAPK signaling pathways comparison no could be development novel diagnostic therapeutical tools PTB control.

Язык: Английский

Процитировано

3
Integrated Analysis of Transcriptome Profiles and lncRNA–miRNA–mRNA Competing Endogenous RNA Regulatory Network to Identify Biological Functional Effects of Genes and Pathways Associated with Johne’s Disease in Dairy Cattle DOI Creative Commons
Farzad Ghafouri,

Vahid Dehghanian Reyhan,

Mostafa Sadeghi

и другие.

Non-Coding RNA, Год журнала: 2024, Номер 10(4), С. 38 - 38

Опубликована: Июнь 28, 2024

Paratuberculosis or Johne’s disease (JD), a chronic granulomatous gastroenteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), causes huge economic losses and reduces animal welfare in dairy cattle herds worldwide. At present, molecular mechanisms biological functions involved immune responses to MAP infection of are not clearly understood. Our purpose was integrate transcriptomic profiles competing endogenous RNA (ceRNA) network analyses identify key messenger RNAs (mRNAs) regulatory regulation peripheral blood mononuclear cells (PBMCs) for cattle. In total, 28 lncRNAs, 42 miRNAs, 370 mRNAs were identified integrating gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses. this regard, we 21 hub genes (CCL20, CCL5, CD40, CSF2, CXCL8, EIF2AK2, FOS, IL10, IL17A, IL1A, IL1B, IRF1, MX2, NFKB1, NFKBIA, PTGS2, SOCS3, TLR4, TNF, TNFAIP3, VCAM1) infection. Furthermore, eight candidate subnets with 29 237 detected through clustering analyses, whereas GO analysis revealed 510, 22, 11 significantly enriched terms related process, function, cellular component categories, respectively. The main metabolic-signaling pathways that included the system defense response, response cytokine, leukocyte migration, T cell activation, bacterium, NOD-like receptor, B NF-kappa B, IL-17, receptor signaling pathways. Contributions transcriptome from MAP-positive MAP-negative sample groups plus ceRNA underlying phenotypic differences intensity pathogenicity JD provided novel insights into associated

Язык: Английский

Процитировано

0