Functional Characterization of Pomegranate CAMTA3 in Cold Stress Responses DOI Creative Commons
Shuangshuang Zhao, Rui Lü,

Lijuan Feng

и другие.

Plants, Год журнала: 2025, Номер 14(5), С. 813 - 813

Опубликована: Март 5, 2025

Cold stress is a significant factor limiting plant growth and development. Pomegranate particularly susceptible to low temperatures. Calmodulin-binding transcriptional activators (CAMTAs) are key regulators of cold tolerance in plants. In this study, we conducted comprehensive analysis the CAMTA family proteins across 12 species, including Punica granatum (pomegranate), using bioinformatic methods. CAMTA3 (PgCAMTA3) was isolated characterized, it demonstrated enhanced when expressed Arabidopsis thaliana. Quantitative real-time PCR (qRT-PCR) showed that expression PgCAMTA3 up-regulated under ABA treatments pomegranates. Two A. thaliana transgenic lines, OE1 OE2, which overexpress PgCAMTA3, were generated through genetic transformation. The overexpression OE2 exhibited higher survival rates stress. Furthermore, enzymatic activity assays revealed peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) OE lines. These antioxidant activities collectively contribute better by providing more effective reactive oxygen species (ROS) scavenging cellular protection mechanisms, confirmed lower levels malondialdehyde (MDA) ROS production. addition, led upregulation AtCBF2, AtNCED3, AtWRKY22, modulated CAMTA3. summary, report role tolerance. Our findings provide valuable insights into CAMATA plants offer new perspectives on molecular mechanisms underlying

Язык: Английский

Functional Characterization of Pomegranate CAMTA3 in Cold Stress Responses DOI Creative Commons
Shuangshuang Zhao, Rui Lü,

Lijuan Feng

и другие.

Plants, Год журнала: 2025, Номер 14(5), С. 813 - 813

Опубликована: Март 5, 2025

Cold stress is a significant factor limiting plant growth and development. Pomegranate particularly susceptible to low temperatures. Calmodulin-binding transcriptional activators (CAMTAs) are key regulators of cold tolerance in plants. In this study, we conducted comprehensive analysis the CAMTA family proteins across 12 species, including Punica granatum (pomegranate), using bioinformatic methods. CAMTA3 (PgCAMTA3) was isolated characterized, it demonstrated enhanced when expressed Arabidopsis thaliana. Quantitative real-time PCR (qRT-PCR) showed that expression PgCAMTA3 up-regulated under ABA treatments pomegranates. Two A. thaliana transgenic lines, OE1 OE2, which overexpress PgCAMTA3, were generated through genetic transformation. The overexpression OE2 exhibited higher survival rates stress. Furthermore, enzymatic activity assays revealed peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) OE lines. These antioxidant activities collectively contribute better by providing more effective reactive oxygen species (ROS) scavenging cellular protection mechanisms, confirmed lower levels malondialdehyde (MDA) ROS production. addition, led upregulation AtCBF2, AtNCED3, AtWRKY22, modulated CAMTA3. summary, report role tolerance. Our findings provide valuable insights into CAMATA plants offer new perspectives on molecular mechanisms underlying

Язык: Английский

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