Neuronal guanine nucleotide exchange factor promotes the axonal growth and cancer cell proliferation via Ephrin-A3/EphA2 axis in lung adenocarcinoma

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 28, 2025

Neural infiltration has been found in various cancers and the infiltrating nerves influence tumor growth dissemination. In non-small cell lung cancer, pan-neuronal marker PGP9.5 was detected by immunohistochemical staining its high expression correlated with poor prognosis. However, existence of nerve fibers mechanism driving neural remains unclear. We first used to assess density patients adenocarcinoma different sizes. Following that, we performed differential analysis univariate Cox prognostic analysis, using public datasets experiments identify gene that triggers is associated cancer progression unfavorable Finally, molecular biology a subcutaneous model were deeply analyze regulates progression. patients, positive within tumors larger than 2 cm diameter significantly higher smaller cm. Bioinformatics suggested NGEF, KIF4A, PABPC1 could be genes trigger are Subsequent co-culture neurons showed increased NGEF cells enhanced axonal neurons. Meanwhile, GSE30219 indicated exhibiting levels sizes, lymph node involvement, reduced overall survival rates. At level mechanisms, knockdown Ephrin-A3 ND7/23 or use ALW-II-41-27 resulted significant decrease neurite outgrowth when co-cultured LA795 cells. animal model, overexpression promoted fibers, these effects inhibited ALW-II-41-27. facilitates through Ephrin-A3/EphA2 pathway, suggesting promising target for disrupting interactions between tumors. Biomaterials focus on anticipated potential treatment option cancer.

Язык: Английский

Unravelling the role of NDUFAF4 in Colon Cancer: Insights from multi-omics analysis

Journal of Proteomics, Год журнала: 2024, Номер 310, С. 105309 - 105309

Опубликована: Сен. 6, 2024

Язык: Английский