Evolution of heat‐shock protein expression underlying adaptive responses to environmental stress

Molecular Ecology, Год журнала: 2018, Номер 27(15), С. 3040 - 3054

Опубликована: Июнь 19, 2018

Abstract Heat‐shock proteins (Hsps) and their cognates are primary mitigators of cell stress. With increasingly severe impacts climate change other human modifications the biosphere, ability heat‐shock system to affect evolutionary fitness in environments outside laboratory evolve response is topic growing importance. Since last major reviews, several advances have occurred. First, demonstrations now include many additional taxa environments. Many these only correlative, however. More importantly, technical “omic” quantification nucleic acids proteins, genomewide association analysis, manipulation genes expression enabled field move beyond correlation. Several consequent already evident: The pathway from gene stress tolerance nature can be extremely complex, mediated through multiple biological processes systems, even species. underlying more numerous, diverse variable than previously appreciated, especially with respect regulatory variation epigenetic changes. limitations (e.g., due trade‐offs) natural selection on become obvious better established. At last, as capacitors, Hsps may distinctive evolution ecological consequences.

Язык: Английский

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De novo assembly of the goldfish ( Carassius auratus ) genome and the evolution of genes after whole-genome duplication

Science Advances, Год журнала: 2019, Номер 5(6)

Опубликована: Июнь 1, 2019

A genomic assembly of the goldfish ( Carrassius auratus ) increases utility in research and agriculture.

Язык: Английский

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Extracellular Vesicles and Exosomes: Insights From Exercise Science

Frontiers in Physiology, Год журнала: 2021, Номер 11

Опубликована: Фев. 1, 2021

The benefits of exercise on health and longevity are well-established, evidence suggests that these effects partially driven by a spectrum bioactive molecules released into circulation during (e.g., factors or 'exerkines'). Recently, extracellular vesicles (EVs), including microvesicles (MVs) exosomes exosome-like (ELVs), were shown to be secreted concomitantly with exerkines. These EVs have therefore been proposed act as cargo carriers 'mediators' intercellular communication. Given findings, there has rapidly growing interest in the role multi-systemic, adaptive response exercise. This review aims summarize our current understanding MVs ELVs, examine their long-term adaptations, highlight main methodological hurdles related blood collection, purification, characterization ELVs.

Язык: Английский

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Development, characterization, and applications of multi-material stereolithography bioprinting

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Фев. 4, 2021

Abstract As a 3D bioprinting technique, hydrogel stereolithography has historically been limited in its ability to capture the spatial heterogeneity that permeates mammalian tissues and dictates structure–function relationships. This limitation stems directly from difficulty of preventing unwanted material mixing when switching between different liquid bioinks. Accordingly, we present development, characterization, application multi-material bioprinter provides controlled selection, yields precise regional feature alignment, minimizes bioink mixing. Fluorescent tracers were first used highlight broad design freedoms afforded by this fabrication strategy, complemented morphometric image analysis validate architectural fidelity. To evaluate bioactivity printed gels, 344SQ lung adenocarcinoma cells core/shell architecture. These exhibited native phenotypic behavior as evidenced apparent proliferation formation spherical multicellular aggregates. Cells also pre-formed aggregates, which appropriately developed invasive protrusions response hTGF-β1. Finally, constructed simplified model intratumoral with two separate sub-populations cells, together grew over 14 days form dense interface. Together, these studies potential probe heterotypic interactions distinct cell types tissue-specific microenvironments.

Язык: Английский

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Transport mechanisms of plant hormones

Current Opinion in Plant Biology, Год журнала: 2021, Номер 63, С. 102055 - 102055

Опубликована: Июнь 5, 2021

Язык: Английский

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The amoeboid state as part of the epithelial-to-mesenchymal transition programme

Trends in Cell Biology, Год журнала: 2021, Номер 32(3), С. 228 - 242

Опубликована: Ноя. 23, 2021

Cell migration is essential for many biological processes, while abnormal cell characteristic of cancer cells. Epithelial cells become motile by undergoing epithelial-to-mesenchymal transition (EMT), and mesenchymal increase speed adopting amoeboid features. This review highlights how behaviour not merely a mode but rather cellular state - within the EMT spectra which survive, invade colonise challenging microenvironments. Molecular biomarkers physicochemical triggers associated with are discussed, including an tumour microenvironment. We reflect on characteristics support metastasis their liabilities could turn into therapeutic opportunities.

Язык: Английский

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Interspecies bacterial competition regulates community assembly in the C. elegans intestine

The ISME Journal, Год журнала: 2021, Номер 15(7), С. 2131 - 2145

Опубликована: Фев. 15, 2021

From insects to mammals, a large variety of animals hold in their intestines complex bacterial communities that play an important role health and disease. To further our understanding how intestinal assemble function, we study the C. elegans microbiota with bottom-up approach by feeding this nematode monocultures as well mixtures two eight species. We find bacteria colonizing monoculture do not always co-cultures due interspecies interactions. Moreover, community diversity increases, ability colonize worm gut becomes less than interactions for determining assembly. explore host-microbe adaptation, compare isolated from non-native isolates, success colonization is determined more species' taxonomy isolation source. Lastly, comparing assembled microbiotas mutants, innate immunity via p38 MAPK pathway decreases abundances yet has little influence on composition. These results highlight interactions, so adaptation or environmental filtering, dominant assembly microbiota.

Язык: Английский

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Molecular mechanisms of adaptive evolution in wild animals and plants

Science China Life Sciences, Год журнала: 2023, Номер 66(3), С. 453 - 495

Опубликована: Янв. 13, 2023

Язык: Английский

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Expanding anchored hybrid enrichment to resolve both deep and shallow relationships within the spider tree of life

BMC Evolutionary Biology, Год журнала: 2016, Номер 16(1)

Опубликована: Окт. 13, 2016

Despite considerable effort, progress in spider molecular systematics has lagged behind many other comparable arthropod groups, thereby hindering family-level resolution, classification, and testing of important macroevolutionary hypotheses. Recently, alternative targeted sequence capture techniques have provided a powerful tool for resolving relationships across the Tree Life. One these approaches, Anchored Hybrid Enrichment (AHE), is designed to recover hundreds unique orthologous loci from genome, both shallow deep-scale evolutionary within non-model systems. Herein we present modification AHE approach that expands its use application spiders, with particular emphasis on infraorder Mygalomorphae. Our aim was design set probes effectively informative at diversity phylogenetic timescales. Following identification putative arthropod-wide loci, utilized homologous transcriptome sequences 17 species all spiders identify exon boundaries. Conserved regions variable flanking were then sought tick three published araneomorph genomes, raw genomic reads two mygalomorph taxa. development 585 target Spider Probe Kit, applied taxonomic depths evaluate performance: deep-level family (33 taxa, 327 loci); generic Euctenizidae (25 403 North American tarantula genus Aphonopelma (83 581 loci). At deepest level, major lineages (the Mesothelae, Mygalomorphae, Araneomorphae) supported high bootstrap support. Strong support also found throughout Euctenizidae, including Aptostichus. As virtually identical topologies inferred Aphonopelma. The first implementation methodology Class Arachnida, holds great promise gathering types quantities data needed accelerate an understanding Life by providing mechanism whereby different researchers can confidently same independent projects, yet allowing synthesis research groups.

Язык: Английский

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The Deep Origin and Recent Loss of Venom Toxin Genes in Rattlesnakes

Current Biology, Год журнала: 2016, Номер 26(18), С. 2434 - 2445

Опубликована: Сен. 1, 2016

Язык: Английский

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