Transcriptome-based analysis reveals chromatin remodeling in post-adult eclosion reconstruction of the insect fat body DOI Creative Commons
Yiying Li, Yuanyuan Hu, Bei Wang

и другие.

Journal of Integrative Agriculture, Год журнала: 2024, Номер unknown

Опубликована: Июнь 1, 2024

The insect fat body is comparable to the liver and adipose tissue in vertebrates, plays a pivotal role energy metabolism, nutrient storage, reproduction. During metamorphosis, disassembled via programmed cell death dissociation. After adult eclosion, reconstructed either by repopulation from remaining juvenile cells or differentiation progenitor cells. This reconstruction prerequisite for initiating extensive synthesis of vitellogenin (Vg), which necessary maturation eggs. Despite its significance, underlying mechanisms this remain inadequately understood. Transcriptome analysis bodies migratory locusts at 0-5 days post emergence revealed 79 genes associated with chromatin remodeling. Weighted gene co-expression network indicated positive correlation between remodeling reconstitution. Protein-protein interaction that brahma, encodes catalytic subunit SWI/SNF complex, crucial post-adult-eclosion development. qRT-PCR demonstrated levels brahma mRNA are progressively increased during previtellogenic stage, then reach peak elevated vitellogenic phase. Furthermore, expressed response gonadotropic hormone (JH). Knockdown led marked reduction Vg expression within body, along arrested ovarian growth. These findings shed light on involvement brahma-mediated JH-stimulated reproduction female locusts.

Язык: Английский

Juvenile Hormone Studies in Drosophila melanogaster DOI Creative Commons
Xiaoshuai Zhang, Sheng Li, Suning Liu

и другие.

Frontiers in Physiology, Год журнала: 2022, Номер 12

Опубликована: Фев. 10, 2022

In the field of insect endocrinology, juvenile hormone (JH) is one most wondrous entomological terms. As a unique sesquiterpenoid produced and released by endocrine gland, corpus allatum (CA), JH critical regulator in multiple developmental physiological processes, such as metamorphosis, reproduction, behavior. Benefited from precise genetic interventions simplicity, fruit fly,

Язык: Английский

Процитировано

47
Endocrine factors modulating vitellogenesis and oogenesis in insects: An update DOI
Jimena Leyria

Molecular and Cellular Endocrinology, Год журнала: 2024, Номер 587, С. 112211 - 112211

Опубликована: Март 15, 2024

Язык: Английский

Процитировано

15
Kr-h1, a Cornerstone Gene in Insect Life History DOI Creative Commons
Qianyu He, Yuanxi Zhang

Frontiers in Physiology, Год журнала: 2022, Номер 13

Опубликована: Апрель 27, 2022

Insect life cycle is coordinated by hormones and their downstream effectors. Krüppel homolog1 (Kr-h1) one of the crucial effectors which mediates actions two critical insects, juvenile hormone (JH) 20-hydroxyecdysone (20E). It a transcription factor with DNA-binding motif eight C 2 H zinc fingers found to be conserved among insect orders. The expression Kr-h1 fluctuant during development high abundance in instars lower levels final instar pupal stage, reappearance adults, governed coordination JH, 20E, miRNAs. dynamic pattern closely linked its function entire insects. Over past several years, accumulating studies have advanced our understanding role development. acts as universal antimetamorphic both hemimetabolous holometabolous species directly inhibiting 20E signaling genes Broad-Complex ( Br-C ) Ecdysone induced protein 93F E93 ), steroidogenic enzyme involved ecdysone biosynthesis. Meanwhile, it promotes vitellogenesis ovarian majority studied In addition, regulates behavioral plasticity caste identity, neuronal morphogenesis, maturation sexual behavior, well embryogenesis metabolic homeostasis. Hence, cornerstone regulator life.

Язык: Английский

Процитировано

36
Juvenile hormone promotes paracellular transport of yolk proteins via remodeling zonula adherens at tricellular junctions in the follicular epithelium DOI Creative Commons
Hongyuan Zheng, Ningbo Wang,

Jiaqi Yun

и другие.

PLoS Genetics, Год журнала: 2022, Номер 18(6), С. e1010292 - e1010292

Опубликована: Июнь 27, 2022

Juvenile hormone (JH) acts as a gonadotrophic stimulating insect vitellogenesis and oogenesis. Paracellular transport of yolk proteins through intercellular channels (patency) in the follicular epithelium is developmentally regulated evolutionarily conserved process during vitellogenesis. However, mechanisms underlying patency opening are poorly understood. Using migratory locust Locusta migratoria model system, we report here that JH-regulated remodeling zonula adherens (ZA), belt-like junction maintaining physical linking between follicle cells controlled patency. JH triggered phosphorylation Partitioning defective protein 3 (Par3) via signaling cascade including G protein-coupled receptor (GPCR), small GTPase Cell division cycle 42 (Cdc42) atypical Protein kinase C (aPKC). Par3 resulted its disassociation from β-Catenin, cytoplasmic partner ZA core component E-Cadherin. Release β-Catenin/E-Cadherin complex caused disassembly at tricellular contacts, consequently leading to enlargement. This study provides new insight into how stimulates egg production inducing paracellular route for vitellogenin crossing barrier.

Язык: Английский

Процитировано

33
Biological Characteristics and Energy Metabolism of Migrating Insects DOI Creative Commons
Xiaokang Li, Yan Zhou, Kongming Wu

и другие.

Metabolites, Год журнала: 2023, Номер 13(3), С. 439 - 439

Опубликована: Март 17, 2023

Through long-distance migration, insects not only find suitable breeding locations and increase the survival space opportunities for population but also facilitate large-scale material, energy, information flow between regions, which is important in maintaining stability of agricultural ecosystems wider natural ecosystems. In this study, we summarize changes biological characteristics such as morphology, ovarian development, reproduction, flight capability during seasonal migration insect. consideration global research work, interaction influence regulation insulin-like juvenile hormone on reproductive activities migrating insects, types energy substances, metabolic processes, processes insect are elaborated. This systematic review latest advances studies biology metabolism will help readers to better understand behavior mechanism migration.

Язык: Английский

Процитировано

18
Egfr signaling promotes juvenile hormone biosynthesis in the German cockroach DOI Creative Commons
Laurent Li,

Caisheng Zhou,

Yumei Chen

и другие.

BMC Biology, Год журнала: 2022, Номер 20(1)

Опубликована: Дек. 13, 2022

Abstract Background In insects, an interplay between the activities of distinct hormones, such as juvenile hormone (JH) and 20-hydroxyecdysone (20E), regulates progression through numerous life history hallmarks. As a crucial endocrine factor, JH is mainly synthesized in corpora allata (CA) to regulate multiple physiological developmental processes, including molting, metamorphosis, reproduction. During last century, significant progress has been achieved elucidating signal transduction pathway, while less made dissecting regulatory mechanism biosynthesis. Previous work shown that receptor tyrosine kinase (RTK) signaling biosynthesis both insects mammals. Here, we performed systematic RNA interference (RNAi) screening identify RTKs involved regulating CA adult Blattella germanica females. Results We found epidermal growth factor (Egfr) required for promoting The Egf ligands Vein Spitz activate Egfr, followed by Ras/Raf/ERK signaling, finally activation downstream transcription Pointed (Pnt). Importantly, Pnt induces transcriptional expression two key enzyme-encoding genes pathway: acid methyltransferase (JHAMT) methyl farnesoate epoxidase (CYP15A1). Dual-luciferase reporter assay shows able promoter region Jhamt . addition, electrophoretic mobility shift confirms directly binds − 941~ 886 nt promoter. Conclusions This study reveals detailed molecular Egfr German cockroach, shedding light on intricate regulation during insect development.

Язык: Английский

Процитировано

16
Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone DOI Open Access
Yan-Xue Li, Xin-Le Kang, Yan-Li Li

и другие.

Опубликована: Март 3, 2025

Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.

Язык: Английский

Процитировано

0
Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone DOI Creative Commons
Yan-Xue Li, Xin-Le Kang, Yan-Li Li

и другие.

eLife, Год журнала: 2025, Номер 13

Опубликована: Март 14, 2025

Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.

Язык: Английский

Процитировано

0
Krüppel homolog 1, a juvenile hormone transcription factor, regulates the reproduction and development of Aedes albopictus(Skuse) (Diptera: Culicidae) DOI Creative Commons
Yahui Chen,

Cheng Wu,

Yuyang Xie

и другие.

Parasitology Research, Год журнала: 2025, Номер 124(3)

Опубликована: Март 1, 2025

The control of Aedes albopictus (Skuse) (Diptera: Culicidae), a vector for several important viral diseases, is crucial mitigating mosquito-borne diseases. This study focused on the Krüppel homolog 1 (Kr-h1) gene, transcription factor in juvenile hormone (JH) signaling, which plays pivotal role inhibiting metamorphosis and promoting adult reproduction. We characterized Kr-h1(AalbKr-h1), identified its eight zinc finger domains, confirmed orthology among insects through phylogenetic analysis. Expression profiling across life stages showed high level expression eggs, late larvae, adults, with minimal pupae. In AalbKr-h1 was most active fat body ovaries. Exposing larvae to JH analogue significantly upregulated both larval stages. RNAi-mediated knockdown Kr-h1 protein reduced egg reproduction, survival rate gene levels. These results provide solid foundation further exploration function potential development novel strategies mosquito prevention

Язык: Английский

Процитировано

0
Juvenile hormone and energy metabolism shape the optimal timing of flight to reproduction transition in migratory locusts DOI

Lin‐Jie Zhang,

Tingting Liu, Ran Gao

и другие.

Insect Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Abstract Migratory insects are capable of long‐distance flight and strong fecundity, but often have finite amounts resources available for these energy‐demanding traits. Although the trade‐off between reproduction has been reported in migratory insects, optimal timing to transition remains largely unknown. Here, using gregarious phase locust Locusta migratoria , we report that 4‐d‐old adult females possessed strongest capacity first gonadotrophic cycle. Tethered assays demonstrated point ending previtellogenesis beginning vitellogenesis, when vitellogenin (Vg) was not yet massively synthesized, flight. Transcriptome metabolome analyses showed glycogen triglyceride were primarily synthesized fat body during previtellogenic stage. Sustained significantly reduced Vg expression levels accompanied by blocked oocyte growth, prolonged preoviposition period declined egg number. In addition, long‐term led juvenile hormone (JH) synthesis genes JHAMT HMGR allatotropin JH metabolism JHE JHEH . Application mimic JH‐deprived at a lower dose conducive contrast, administration higher doses stimulated vitellogenesis production suppressed capacity. Our results suggest along with energy regulate switch locust. The findings shed new light on regulation reproduction, as well sustainable control locusts.

Язык: Английский

Процитировано

0