A Cold-Induced LEA3 Protein, DohD, Confers Cryoprotective Protection Against Low-Temperature Stress in Deinococcus radiodurans
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(8), С. 3511 - 3511
Опубликована: Апрель 9, 2025
Deinococcus
radiodurans
is
a
remarkably
unique
microorganism,
exhibiting
extraordinary
tolerance
to
extreme
conditions
such
as
ionizing
radiation,
ultraviolet
light,
and
desiccation.
However,
the
response
mechanisms
of
D.
under
low-temperature
stress
remain
largely
unexplored
have
yet
be
fully
elucidated.
The
DohD
protein
hydrophilic
member
late
embryogenesis
abundant
3
(LEA3)
family
radiodurans,
playing
pivotal
role
in
abiotic
adaptation.
Bioinformatics
analysis
revealed
that
contains
tandem
repeats
disordered
domains,
with
high
α-helix
content
(91.41%).
Furthermore,
exhibits
extremely
low
homology
other
proteins,
highlighting
its
uniqueness
radiodurans.
Under
(15
°C),
expression
dohD
was
significantly
upregulated
(5-fold),
regulated
by
dual
mechanism
involving
positive
control
DrRRA
negative
regulation
Csp.
Circular
dichroism
spectroscopy
unveiled
temperature-dependent
structural
plasticity:
temperature
increased
from
0
°C
50°
C,
decreased
23.5%
18.7%,
while
antiparallel
β-sheet
31.3%
50.8%.
This
suggests
an
interconversion
strategy
for
thermal
Additionally,
deletion
impaired
cold,
desiccation,
oxidative,
high-salt
stresses,
accompanied
reduced
activities
antioxidant
enzymes
(SOD,
CAT,
POD)
downregulation
related
gene
expression.
study
elucidates
multifunctional
resistance
through
dynamics,
transcriptional
regulation,
redox
homeostasis,
providing
valuable
insights
into
adaptation
extremophiles.
Язык: Английский
Phyletic Patterns of Bacterial Growth Temperature in Pseudomonas and Paenibacillus Reveals Gradual and Sporadic Evolution Towards Cold Adaptation
ISME Communications,
Год журнала:
2024,
Номер
4(1)
Опубликована: Янв. 1, 2024
Abstract
Bacterial
species
adapt
to
cold
environments
with
diverse
molecular
mechanisms
enabling
their
growth
under
low
ambient
temperature.
The
emergence
of
cold-adapted
at
macro-evolutionary
scale,
however,
has
not
been
systematically
explored.
In
this
study,
we
performed
phylogenetic
analysis
on
the
temperature
traits
in
genera
that
occupy
broad
environmental
and
host
niches
contain
known
species.
Our
results
demonstrate
genus
Pseudomonas,
formed
a
distinct
conserved
clade,
whereas
Paenibacillus,
were
sporadically
distributed
throughout
tree.
clade
Pseudomonas
exhibited
genome-wide
signatures
adaptation
possessed
clade-specific
genes.
This
indicates
there
are
evolutionary
patterns
divergence
among
different
bacterial
genera.
Язык: Английский