Paving Way for a Paradigm Shift in Oncology: Curing Cancer by Loving It? DOI Creative Commons
Vuk Uskoković

Micro, Год журнала: 2023, Номер 3(3), С. 749 - 762

Опубликована: Сен. 21, 2023

Plateaus in the efficacy of traditional methods for treatment cancer reached last decades call exploration alternative models as their potential clinical complements. Here, classical view a tissue that is to be eradicated by describable compendium militaristic metaphors being challenged with provocative idea: what if can cured love condensed down level molecular and cell biology? Correspondingly, idea mimics traits objects its affection helps them grow was translated biology incorporating anti-apoptotic properties healthy cells promoting tumorigenesis cancerous cells. Both indirect direct co-culture two types demonstrated hindered growth relative primary counterparts when these cellular modifications inspired were implemented. The experimental reported here are emphasized crude simplistic derived from may best treated loved at levels. More comprehensive effective emanate continued expansion intriguing innovative avenue management proposed here.

Язык: Английский

A (RP)UHPLC/UV analytical method to quantify dsRNA during the mRNA vaccine manufacturing process DOI Creative Commons
Sara Sousa Rosa,

Shuran Zhang,

Yustika Sari

и другие.

Analytical Methods, Год журнала: 2024, Номер 16(30), С. 5146 - 5153

Опубликована: Янв. 1, 2024

Development of an HPLC method to quantify dsRNA, a product related impurity, produced during mRNA vaccine manufacturing. This allows dsRNA in single samples under 30 min.

Язык: Английский

Процитировано

0
Integrated multi-omics profiling reveals the ZZZ3/CD70 axis is a super-enhancer-driven regulator of diffuse large B-cell lymphoma cell-natural killer cell interactions DOI Creative Commons

Xi Li,

Ju-Ya Cui,

Wang Liao

и другие.

Experimental Biology and Medicine, Год журнала: 2024, Номер 249

Опубликована: Сен. 23, 2024

Tumor immune microenvironment is crucial for diffuse large B-cell lymphoma (DLBCL) development. However, the mechanisms by which super-enhancers (SEs) regulate interactions between DLBCL cells and tumor-infiltrating remains largely unknown. This study aimed to investigate role of SE-controlled genes in regulating cells. Single-cell RNA-seq, bulk RNA-seq H3K27ac ChIP-seq data were downloaded from Heidelberg Open Research Data database Gene Expression Omnibus database. HOMER algorithm Seurat package R used bioinformatics analysis. Cell proliferation lactate dehydrogenase (LDH) release was detected MTS LDH assays, respectively. Interaction B cell cluster CD8 + T NK most obviously enhanced DLBCL, with CD70-CD27, MIF-CD74/CXCR2 complex, MIF-CD74/CD44 complex CCL3-CCR5 significantly increased. sub-cluster showed strongest interaction cluster. ZZZ3 upregulated transcription CD70 binding its SE. Silencing DOHH2 promoted co-cultured NK92 cells, counteracted overexpression silencing combined PD-L1 blockade In conclusion, inhibited killing infiltrating ZZZ3/CD70 axis. Targeting axis expected be a promising strategy treatment.

Язык: Английский

Процитировано

0
Inhibition of MC38 colon cancer growth by multicomponent chemoimmunotherapy with anti-IL-10R antibodies, HES-MTX nanoconjugate, depends on application of IL-12, IL-15 or IL-18 secreting dendritic cell vaccines DOI Creative Commons
Katarzyna Węgierek‐Ciura, Jagoda Mierzejewska, Agnieszka Szczygieł

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Июль 20, 2023

Background The tumor microenvironment (TME) provides a conducive environment for the growth and survival of tumors. Negative factors present in TME, such as IL-10, may limit effectiveness cellular vaccines based on dendritic cells, therefore, it is important to control its effect. influence IL-10 immune cells can be abolished e.g., by using antibodies against receptor this cytokine - anti-IL-10R. Furthermore, anticancer activity enhanced modifying them produce proinflammatory cytokines, IL-12, IL-15 or IL-18. Additionally, an immunomodulatory dose methotrexate hydroxyethyl starch (HES-MTX) nanoconjugate stimulate effector eliminate regulatory T which should enhance antitumor action immunotherapy DC vaccines. main aim our study was determine whether HES-MTX administered before with anti-IL-10R would change effect overproducing IL-15, Methods modified DCs checked two therapeutic protocols addition anti-IL10R chemoimmunotherapy antibodies. inhibition therapy inducing specific response were determined analyzing lymphoid myeloid cell populations nodules, restimulated splenocytes. Results conclusions Using multiple administrations capable cytokines IL-18 created optimal conditions effective these murine colon carcinoma MC38 model. applied caused highest group receiving DC/IL-15/IL-15Rα/TAg + DC/IL-18/TAg at level 72.4%. use resulted cytotoxic increase both immuno- chemoimmunotherapy. However, greatest potential observed tissue splenocytes obtained from mice two- three-component course combined application. Thus, designed treatment schedule promising therapy.

Язык: Английский

Процитировано

0
Development and validation of a machine learning model for bone metastasis in prostate cancer: Based on inflammatory and nutritional indicators DOI Creative Commons

Tongtong Jin,

Jingjing An,

Wangjian Wu

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Июль 26, 2023

Abstract Purpose Application of machine learning in bone metastasis prostate cancer based on inflammation and nutritional indicators. Methods Retrospective analysis the clinical data patients with initially diagnosed Department Urology Gansu Provincial People's Hospital from June 2017 to 2022. Logistic regression (LR) least absolute shrinkage selection operator (LASSO) are used jointly screened model features. The filtered features incorporated into algorithms including LR, random forest (RF), extreme gradient boosting (XGBoost), naive nayes (NB), k-nearest neighbor (KNN), decision tree (DT), develop models. Results A total 404 were finally screened. Gleason score, T stage, N PSA ALP as for modeling. average AUC 5-fold cross-validation each training set is: LR (AUC = 0.9054), RF 0.9032), NB 0.8961), KNN 0.8704), DT 0.8526), XGBoost 0.8066). test is 0.9390, 95%CI: 0.8760 ~ 1), 0.9290, 0.8718 0.9861), 0.9268, 0.8615 0.9920), 0.9212, 0.8506 0.9917), 0.8292, 0.7442 0.9141), 0.8057, 0.7100 0.9014). comprehensive evaluation DeLong among different models metric shows that best study. Conclusion was established, it observed indicators such nutrition had a weak correlation metastasis.

Язык: Английский

Процитировано

0
Paving Way for a Paradigm Shift in Oncology: Curing Cancer by Loving It? DOI Creative Commons
Vuk Uskoković

Micro, Год журнала: 2023, Номер 3(3), С. 749 - 762

Опубликована: Сен. 21, 2023

Plateaus in the efficacy of traditional methods for treatment cancer reached last decades call exploration alternative models as their potential clinical complements. Here, classical view a tissue that is to be eradicated by describable compendium militaristic metaphors being challenged with provocative idea: what if can cured love condensed down level molecular and cell biology? Correspondingly, idea mimics traits objects its affection helps them grow was translated biology incorporating anti-apoptotic properties healthy cells promoting tumorigenesis cancerous cells. Both indirect direct co-culture two types demonstrated hindered growth relative primary counterparts when these cellular modifications inspired were implemented. The experimental reported here are emphasized crude simplistic derived from may best treated loved at levels. More comprehensive effective emanate continued expansion intriguing innovative avenue management proposed here.

Язык: Английский

Процитировано

0