Functional Characterization of Two Polymerizing Glycosyltransferases for the Addition of N-Acetyl-d-galactosamine to the Capsular Polysaccharide of Campylobacter jejuni
Biochemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 24, 2025
The
exterior
surface
of
the
human
pathogen
Campylobacter
jejuni
is
coated
with
a
capsular
polysaccharide
(CPS)
that
consists
repeating
sequence
2–5
different
sugars
can
be
modified
various
molecular
decorations.
In
HS:2
serotype
from
strain
NCTC
11168,
unit
within
CPS
composed
d-ribose,
N-acetyl-d-galactosamine,
and
d-glucuronic
acid
further
amidated
either
serinol
or
ethanolamine.
moiety
also
decorated
d-glycero-l-gluco-heptose.
Here,
we
show
two
GT2
glycosyltransferases
catalyze
transfer
N-acetyl-d-galactosamine
UDP-NAc-d-galactosamine
furanoside
to
C4-hydroxyl
group
d-glucuronamide
at
growing
end
chain.
Catalytic
activity
was
not
observed
glycosides
acid,
thus,
C6-carboxylate
must
prior
chain
elongation.
One
these
enzymes
comprises
N-terminal
domain
Cj1438
(residues
1–325)
other
Cj1434
1–327).
These
are
∼87%
identical
in
sequence,
but
it
clear
why
there
same
gene
cluster
apparently
reaction.
This
discovery
represents
second
polymerizing
glycosyltransferase
has
been
isolated
functionally
characterized
for
biosynthesis
C.
jejuni.
Язык: Английский
A novel mathematical model for studying antimicrobial interactions against viable but non-culturable Campylobacter jejuni in the poultry product processing environment
Food Microbiology,
Год журнала:
2025,
Номер
128, С. 104740 - 104740
Опубликована: Фев. 1, 2025
Язык: Английский
Outer membrane vesicles in gram-negative bacteria and its correlation with pathogenesis
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 1, 2025
There
is
a
widespread
distribution
of
gram-negative
bacteria
worldwide,
which
are
responsible
for
the
deaths
numerous
patients
each
year.
The
illnesses
they
cause
can
be
localized
and
systemic,
these
possess
several
key
virulence
factors
that
contribute
to
their
pathogenicity.
In
recent
years,
distinct
mechanisms
pathogenesis
have
evolved
remain
largely
unknown
scientists
medical
experts.
Among
these,
outer
membrane
vesicles
(OMVs)
undoubtedly
one
most
significant
influencing
virulence.
OMVs
contain
various
bacterial
compounds
diverse
effects
on
host
organisms
immune
system,
potentially
exacerbating
disease
inflammation
while
evading
responses.
This
review
comprehensively
examines
role
in
pathogenesis,
interaction
with
cells,
potential
biomedical
applications.
Understanding
molecular
governing
OMV
biogenesis
function
could
pave
way
novel
antimicrobial
strategies
therapeutic
interventions.
Язык: Английский
Mechanistic concepts involved in biofilm associated processes of Campylobacter jejuni: persistence and inhibition in poultry environments
Poultry Science,
Год журнала:
2024,
Номер
103(12), С. 104328 - 104328
Опубликована: Сен. 13, 2024
Campylobacter
species,
predominantly
jejuni,
remains
a
significant
zoonotic
pathogen
worldwide,
with
the
poultry
sector
being
primary
vector
for
human
transmission.
In
recent
years.
there
has
been
notable
rise
in
incidence
of
campylobacteriosis,
necessitating
deeper
understanding
pathogen's
survival
mechanisms
and
transmission
dynamics.
Biofilm
presence
significantly
contributes
to
C.
jejuni
persistence
subsequent
food
product
contamination,
this
review
describes
intricate
processes
involved
biofilm
formation.
The
ability
form
biofilms
on
various
surfaces,
including
stainless
steel,
plastic,
glass,
is
critical
strategy.
biofilms,
their
remarkable
resilience,
protect
from
environmental
stresses
such
as
desiccation,
pH
extremes,
biocides
sanitizing
agents.
This
explores
molecular
genetic
formation,
highlighting
regulatory
genes
motility,
chemotaxis,
stress
responses.
Flagellar
proteins,
particularly
flaA,
flaB,
flaG,
adhesins
like
cadF
flpA,
are
identified
main
components
development.
role
mixed-species
where
integrates
into
existing
other
bacteria
enhance
also
discussed.
considers
alternative
interventions
control
production,
context
increasing
antibiotic
resistance.
It
effectiveness
prebiotics,
probiotics,
synbiotics,
bacteriocins,
bacteriophages,
vaccines,
organic
acids,
focus
action
reducing
bacterial
colonization
Studies
show
that
mixtures
acids
compounds
Carvacrol
Eugenol
downregulate
linked
motility
adhesion,
thereby
disrupting
integrity.
discusses
impact
factors,
temperature
oxygen
levels
providing
insights
how
industrial
conditions
can
be
manipulated
reduce
contamination.
paper
need
multifaceted
approach
poultry,
integrating
practical
interventions.
By
advancing
our
dynamics
gene
regulation,
we
aim
inform
development
more
effective
strategies
safety
public
health.
Язык: Английский
Consortium of Lactobacillus crispatus 2029 and Ligilactobacillus salivarius 7247 Strains Shows In Vitro Bactericidal Effect on Campylobacter jejuni and, in Combination with Prebiotic, Protects Against Intestinal Barrier Dysfunction
Antibiotics,
Год журнала:
2024,
Номер
13(12), С. 1143 - 1143
Опубликована: Ноя. 28, 2024
Background/Objectives:
Campylobacter
jejuni
(CJ)
is
the
etiological
agent
of
world’s
most
common
intestinal
infectious
food-borne
disease,
ranging
from
mild
symptoms
to
fatal
outcomes.
The
development
innovative
synbiotics
that
inhibit
adhesion
and
reproduction
multidrug-resistant
(MDR)
CJ
in
animals
humans,
thereby
preserving
homeostasis,
relevant.
We
have
created
a
synbiotic
based
on
consortium
Lactobacillus
crispatus
2029
(LC2029),
Ligilactobacillus
salivarius
7247
(LS7247),
mannan-rich
prebiotic
(Actigen®).
purpose
this
work
was
study
vitro
anti-adhesive
antagonistic
activities
against
MDR
strains,
along
with
its
role
preventing
barrier
dysfunction,
which
disrupts
homeostasis.
Methods:
A
complex
microbiological,
immunological,
molecular
biological
methods
used.
ability
LC2029
LS7247
promote
homeostasis
assessed
by
effectiveness
controlling
CJ-induced
TLR4
activation,
secretion
pro-inflammatory
cytokines,
production
alkaline
phosphatase
(IAP).
Results:
All
strains
showed
marked
human
Caco-2,
pig
IPEC-J2,
chicken
CPCE,
bovine
BPCE
enterocytes.
For
first
time,
we
found
cell-free
culture
supernatant
(CFS)
exhibit
an
additive
effect
inhibiting
animal
CFS
increased
permeability
outer
inner
membranes
cells,
led
extracellular
leakage
ATP
provided
access
peptidoglycan
pathogen
for
peptidoglycan-degrading
bacteriocins
nisin
enterolysin
produced
LS7247.
bactericidal
strains.
Co-cultivation
resulted
decrease
viability
6
log.
prevented
growth
mRNA
expression
inhibited
increase
IL-8
TNF-α
enterocytes,
maintained
transepithelial
electrical
resistance
enterocyte
monolayers,
paracellular
zonulin
secretion.
stimulated
IAP
Actigen
represent
new
synergistic
anti-CJ
properties
prevents
dysfunction
preserves
Conclusions:
These
data
highlight
potential
using
as
preventive
strategy
creating
feed
additives
functional
nutrition
products
it
combat
prevalence
campylobacteriosis
caused
humans.
Язык: Английский
Intricate Structure–Function Relationships: The Case of the HtrA Family Proteins from Gram-Negative Bacteria
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 13182 - 13182
Опубликована: Дек. 7, 2024
Proteolytic
enzymes
play
key
roles
in
living
organisms.
Because
of
their
potentially
destructive
action
degrading
other
proteins,
activity
must
be
very
tightly
controlled.
The
evolutionarily
conserved
proteins
the
HtrA
family
are
an
excellent
example
illustrating
strategies
for
regulating
enzymatic
activity,
enabling
protease
activation
response
to
appropriate
signal,
and
protecting
against
uncontrolled
proteolysis.
homologs
virulence
many
Gram-negative
bacterial
pathogens,
they
subject
intense
investigation
as
potential
therapeutic
targets.
Model
from
bacterium
Язык: Английский