Novel fibro-inflammatory biomarkers associated with disease activity in patients with Crohn’s disease

Expert Review of Gastroenterology & Hepatology, Год журнала: 2023, Номер 17(6), С. 575 - 587

Опубликована: Май 11, 2023

Crohn's disease (CD) is a complex disease, and assessing activity challenging due to pathobiologic process e.g. ECM remodeling, mucosal damage, intestinal fibrosis, which greatly limits current assessments through endoscopy imaging techniques.The review highlights the importance of novel biomarkers reflecting remodeling immune cell that accurately reflect CD progression. Such could include collagen formation degradation fragments serum fragment calprotectin, neutrophil activity. A new concept, fibro-inflammation, also introduced in review, all aspects such as inflammation, tissue fibrosis resolution, should be assessed. PubMed searches performed from July 2022 - November provided scientific information included review.Current data suggest may sustain exacerbate chronic leading non-response anti-inflammatory treatments. Therefore, evaluating different stages fibro-inflammatory done clinical setting considered for trials. This approach will help assess activity, better management treatment CD.

Язык: Английский

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Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts’ response to fibrotic growth factors

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Июнь 9, 2023

Systemic Sclerosis (SSc) hallmark is skin fibrosis, but up to 80% of the patients have fibrotic involvement in pulmonary system. Antifibrotic drugs which failed a general SSc population now been approved with SSc-associated interstitial lung disease (ILD). This indicates that progression and regulation fibroblasts likely depend on local factors specific tissue type. study investigated difference between dermal setting, mimicking extracellular matrix. Primary healthy were grown crowded environment stimulated TGF-β1 PDGF-AB. The viability, morphology, migration capacity, matrix formation, gene expression assessed: only increased viability fibroblasts. PDGF-AB capacity while fully migrated. morphology was different without stimulation. formation type III collagen fibroblasts, it trend VI opposite after exhibit response profiles PDGF-AB; this suggests drivers fibrosis are tissue-dependent, needs be considered drug development.

Язык: Английский

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Fibroblast activation protein and disease severity, progression, and survival in idiopathic pulmonary fibrosis

Scandinavian Journal of Immunology, Год журнала: 2024, Номер 100(3)

Опубликована: Июнь 7, 2024

Abstract Idiopathic pulmonary fibrosis (IPF) is characterized by progressive in the lungs. Activated fibroblasts play a central role fibrogenesis and express fibroblast activation protein α. A truncated, soluble form (sFAP) can be measured blood potential novel biomarker of disease activity. The aim was to study association between sFAP clinical, radiological, histopathological measures severity, progression, survival prospective, multicentre, real‐world cohort patients with IPF. Patients IPF were recruited from tertiary interstitial lung centres Denmark followed for up 3 years. Baseline serum levels ELISA compared healthy controls. Pulmonary function tests, 6‐minute walk test quality life performed at baseline during follow‐up. included 149 Median 49.6 ng/mL (IQR: 43.1–61.6 ng/mL) controls 73.8 62.1–92.0 ng/mL). Continuous not associated progression or ( p > 0.05). After dichotomization below above mean + 2 SD controls, higher lower FVC % predicted follow‐up < 0.01). Higher than normal longitudinal changes predicted, but did show clear associations other parameters. As such, has limited use as

Язык: Английский

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Quantitative and qualitative analysis of pulmonary arterial hypertension fibrosis using wide-field second harmonic generation microscopy

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Май 5, 2022

We demonstrated that wide-field second harmonic generation (SHG) microscopy of lung tissue in combination with quantitative analysis SHG images is a powerful tool for fast and label-free visualization the fibrosis pathogenesis pulmonary arterial hypertension (PAH). Statistical revealed changes collagen content morphology during monocrotaline-induced PAH progression rats. First order statistics disclosed dependence overproduction on time, indicated tightening fiber network around blood vessels their spreading into alveolar region. Fourier enhancement orientation was followed its subsequent reduction at terminating phase disease. Proposed approach has potential assessing interstitial disease, after lung(s) transplantation, cancer, etc.

Язык: Английский

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Geneticin ameliorates pulmonary fibrosis by attenuating the TGF-β/Smad via modulating AMPK/SIRT1 signaling

Life Sciences, Год журнала: 2024, Номер 346, С. 122626 - 122626

Опубликована: Апрель 12, 2024

Язык: Английский

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