Role of microRNAs in Immune Regulation with Translational and Clinical Applications

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(3), С. 1942 - 1942

Опубликована: Фев. 5, 2024

MicroRNAs (miRNAs) are 19-23 nucleotide long, evolutionarily conserved noncoding RNA molecules that regulate gene expression at the post-transcriptional level. In this review, involvement of miRNAs is summarized in differentiation and function immune cells, anti-infective responses, immunodeficiencies autoimmune diseases. Roles anticancer immunity transplantation solid organs hematopoietic stem cells also discussed. Major focus put on translational clinical applications miRNAs, including establishment noninvasive biomarkers for differential diagnosis prediction prognosis. Patient selection response to biological therapy one most promising fields application. Replacement or inhibition has enormous therapeutic potential, with constantly expanding possibilities. Although important challenges still await solutions, evaluation miRNA fingerprints may contribute an increasingly personalized management dysregulation a remarkable reduction toxicity treatment side effects. More detailed knowledge molecular effects physical exercise nutrition system facilitate self-tailored lifestyle recommendations advances prevention.

Язык: Английский

---

Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications

Journal of Clinical Medicine, Год журнала: 2023, Номер 12(13), С. 4253 - 4253

Опубликована: Июнь 25, 2023

The role of mitochondria in post coronavirus disease 2019 (post-COVID-19) complications is unclear, especially the long-term pulmonary complications. This study aims to investigate association between post-COVID-19 and mitochondrial regulatory proteins context oxidative stress.Patients who had recovered from COVID-19 were enrolled. According evidence persistent interstitial lung lesions on computed tomography (CT), patients divided into a group (P(+)) control without (P(-)). We randomly selected 80 for investigation (40 subjects each group). Biomarkers levels determined by enzyme-linked immunosorbent assay (ELISA).The serum concentrations significantly higher P(+) group, including PTEN-induced kinase 1 (PINK1): 1.62 [1.02-2.29] ng/mL vs. 1.34 [0.94-1.74] (p = 0.046); Dynamin-1-like protein (DNM1L): 1.6 [0.9-2.4] IQR 0.9 [0.5-1.6] 0.004); Mitofusin-2 (MFN2): 0.3 [0.2-0.5] 0.2 [0.1-0.3] 0.001). Patients also chemokine ligand 18 (PARC, CCL18), IL-6, tumour necrosis factor-alpha (TNF-α) cytokines than P(-) group. concentration interferon alpha (IFN-α) was decreased Furthermore, we observed statistically significant correlations advanced glycation end product (sRAGE) TNF-α (Pearson's factor R 0.637; p < 0.001) DNM1L IFN-α 0.501; 0.002) patients.Elevated biomarkers with indicate their possible pathobiology sequelae. Oxidative stress associated immune response inflammation after COVID-19. could be promising biomarker predicting may potential target therapeutic intervention

Язык: Английский

---

Revisiting the role of MicroRNAs in the pathogenesis of idiopathic pulmonary fibrosis

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Окт. 16, 2024

Idiopathic pulmonary fibrosis (IPF) is a prevalent chronic disease characterized by alveolar epithelial cell damage, fibroblast proliferation and activation, excessive extracellular matrix deposition, abnormal epithelial-mesenchymal transition (EMT), resulting in tissue remodeling irreversible structural distortion. The mortality rate of IPF very high, with median survival time 2–3 years after diagnosis. exact cause remains unknown, but increasing evidence supports the central role epigenetic changes, particularly microRNA (miRNA), IPF. Approximately 10% miRNAs lung exhibit differential expression compared to normal tissue. Diverse miRNA phenotypes exert either pro-fibrotic or anti-fibrotic influence on progression In context IPF, factors such as DNA methylation long non-coding RNAs (lncRNAs) regulate differentially expressed miRNAs, which turn modulate various signaling pathways implicated this process, including transforming growth factor-β1 (TGF-β1)/Smad, mitogen-activated protein kinase (MAPK), phosphatidylinositol-3-kinase/protein B (PI3K/AKT) pathways. Therefore, review presents epidemiology discusses multifaceted regulatory roles explores impact through pathways, TGF-β1/Smad pathway its constituent structures. Consequently, we investigate potential for targeting treatment thereby contributing advancements research.

Язык: Английский

---

Predictive value and mechanism of lncRNA MANCR for pediatric severe pneumonia via miR-20a-5p / MAPK1 axis

Immunobiology, Год журнала: 2025, Номер 230(2), С. 152871 - 152871

Опубликована: Янв. 22, 2025

Язык: Английский

---

miR-27a-5p, miR-21-5p, miR-1246 and miR-4508: a candidate microRNA signature in the protection and regulation of viral infection in mild COVID-19

Molecular Medicine, Год журнала: 2025, Номер 31(1)

Опубликована: Март 15, 2025

Abstract MicroRNAs (miRNAs) are gene regulators essential for cell homeostasis, their alteration is related to a pathological state, including infectious diseases like COVID-19. Identifying an altered profile of circulating miRNAs in mild COVID-19 may enhance our knowledge the pathogenesis SARS-CoV-2 and range clinical phenotypes. In present study, miRNA screening was performed by Next Generation Sequencing (NGS), expression levels 13 resulting were validated through RT-qPCR serum 40 cases compared 29 non-infected individuals. An in-silico analysis detect target genes pathways. From miRNAs, miR-1246 ( p < 0.001), miR-423-5p miR-21-5p = 0.005), miR-146a-5p miR-4508 miR-629-5p miR-210-3p 0.002) found downregulated infected Only miR-27a-5p overexpressed subjects with 0.013) associated infection 0.010). The KEGG pathways GO revealed that differentially expressed viral processes or immunological pathways: acts on TGF-beta pathway; targets SMAD7 , which inflammatory response lung; p53 ICAM2 . conclusion, most relevant miR-21-5p, differently forms higher observed suggest protective effect against severe disease. Reduced prevent pulmonary inflammation progression fibrosis. downregulation conduct correct control infection. Moreover, might be suitable biomarker early stages

Язык: Английский

---

miR-200 Family: Gatekeepers of Fibrinolytic Regulation in Lung Pathologies during Acute Lung injury

Archives of Biochemistry and Biophysics, Год журнала: 2025, Номер unknown, С. 110398 - 110398

Опубликована: Март 1, 2025

Язык: Английский

---

Key Amniotic Fluid miRNAs as Promising Target Molecules for the Antenatal Prevention of Pulmonary Hypoplasia Associated with Congenital Diaphragmatic Hernia

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3872 - 3872

Опубликована: Апрель 19, 2025

Congenital diaphragmatic hernia (CDH) remains associated with high morbidity and mortality, primarily due to pulmonary hypoplasia hypertension. Current antenatal diagnostic methods, such as ultrasound magnetic resonance imaging (MRI), are unable assess the severity of defects in lung vascular structures, which critical determinants diverse phenotypes CDH. Aberrant epigenetic regulation development during gestation is believed play a significant role pathogenesis In this study, we aimed identify miRNA patterns amniotic fluid capable categorizing CDH-fetuses for personalized selection effective treatment strategies at and/or postnatal stages. Using deep sequencing quantitative real-time polymerase chain reaction (PCR), identified set miRNAs—miR-485-3p, miR-320b, miR-320a-3p, miR-221-3p, miR-200b-3p, miR-100-5p, miR-92a-3p, miR-30c-5p, miR-26a-5p, let-7c-5p—whose reduced expression 19–24 weeks allowed us categorize fetuses CDH into two distinct groups: one significantly different from control group (non-CDH) other closely resembling it. Notably, no correlations were found between content these miRNAs assessed by or MRI. However, there was positive correlation level each that whose ensuring proper bronchopulmonary tissue structure prenatal development—as well its therapeutic potential CDH-associated hypoplastic lungs—has been previously demonstrated. These findings lay groundwork future genetically engineered drug formulations designed endotracheal administration correct abnormal levels mitigate progression hypertension CDH-fetuses.

Язык: Английский

---

Trigonelline mitigates bleomycin-induced pulmonary inflammation and fibrosis: Insight into NLRP3 inflammasome and SPHK1/S1P/Hippo signaling modulation

Life Sciences, Год журнала: 2023, Номер 336, С. 122272 - 122272

Опубликована: Ноя. 18, 2023

Язык: Английский

---

miR-486-5p predicted adverse outcomes of SCAP and regulated K. pneumonia infection via FOXO1

BMC Immunology, Год журнала: 2024, Номер 25(1)

Опубликована: Июнь 4, 2024

Abstract Purpose Severe community-acquired pneumonia (SCAP) is a common respiratory system disease with rapid development and high mortality. Exploring effective biomarkers for early detection prediction of SCAP urgent need. The function miR-486-5p in diagnosis prognosis was evaluated to identify promising biomarker SCAP. Patients methods serum 83 patients SCAP, 52 healthy individuals, 68 mild CAP (MCAP) were analyzed by PCR. ROC analysis estimated screening the Kaplan-Meier Cox regression analyses predictive value miR-486-5p. risk factors MCAP developing assessed logistic analysis. alveolar epithelial cell treated Klebsiella mimic occurrence targeting mechanism underlying luciferase reporter assay. Results Upregulated screened from individuals sensitivity specificity. Increasing predicted poor outcomes served as factor into K. induced suppressed proliferation, significant inflammation oxidative stress cells, silencing attenuated it. negatively regulated FOXO1, knockdown FOXO1 reversed effect -treated cells. Conclusion acted monitoring predicting malignancy MCAP. Silencing alleviated via modulating FOXO1.

Язык: Английский

---

The relationship between microRNAs and COVID-19 complications

Non-coding RNA Research, Год журнала: 2024, Номер 10, С. 16 - 24

Опубликована: Авг. 23, 2024

Язык: Английский

---