Clinical application of acute Q fever -induced systemic capillary leak syndrome in a patient by using metagenomic next-generation sequencing: a case report and literature review
BMC Infectious Diseases,
Год журнала:
2025,
Номер
25(1)
Опубликована: Март 2, 2025
Query
fever
(Q
fever),
a
zoonotic
disease,
caused
by
Coxiella
burnetii,
is
an
infectious
disease
that
has
long
been
considered
rare
and
regionally
restricted
disease.
It
can
be
responsible
for
endocarditis
endovascular
infections.
Systemic
capillary
leak
syndrome
(SCLS),
of
unknown
etiology
most
commonly
develops
in
adults
50–70
years
age,
diagnosed
clinically
based
on
characteristic
symptomatic
triad
hypotension,
hemoconcentration
(elevated
hemoglobin
or
hematocrit),
serum
hypoalbuminemia
resulting
from
fluid
extravasation.
Although
Q
increasingly
recognized
reported
recent
years,
the
treatment
complicated
SCLS,
with
etiological
diagnosis
aided
metagenomic
next-generation
sequencing
(mNGS),
remains
uncommon.
This
report
describes
case
acute
concurrent
SCLS
54-year-old
male
who
worked
slaughterhouse.
The
patient
presented
fever,
chest
tightness,
shortness
breath,
accompanied
severe
headache.
His
condition
rapidly
deteriorated,
leading
to
generalized
weakness,
hypotension.
Due
respiratory
failure
shock,
he
was
admitted
intensive
care
unit
(ICU)
treatment.
Despite
empirical
antibiotic
therapy
along
resuscitation,
his
blood
pressure
continued
decline,
metabolic
acidosis
distress
worsened.
As
failed
improve,
tracheal
intubation
performed.
mNGS
detected
both
burnetii
BALF
samples.
Based
results,
started
doxycycline,
alongside
penicillin
antibiotics,
vasopressors,
continuous
renal
replacement
(CRRT).
patient's
gradually
improved,
discharged
home
after
12
days
At
90-day
follow-up,
had
nearly
fully
recovered
pre-illness
status.
plays
crucial
role
assisting
which
enables
timely
underlying
triggering
SCLS.
This,
combined
restrictive
resuscitation
strategies,
essential
improving
outcomes.
Язык: Английский
Academic and clinical perspectives of metagenome sequencing as a diagnostic tool for infectious disease: an interpretive phenomenological study
BMC Infectious Diseases,
Год журнала:
2025,
Номер
25(1)
Опубликована: Март 31, 2025
Abstract
Background
Effective
infectious
disease
diagnostics
(IDD)
are
vital
for
informing
clinical
decision-making
regarding
the
treatment
and
patient
management
of
infections.
In
England,
conventional
methods
rely
upon
culture-dependent
techniques,
there
has
been
little
shift
in
acceptance
integration
culture-independent
sequencing
into
routine
IDD.
This
study
explored
stakeholders’
experiences
within
IDD,
including
those
working
settings
conducting
research
at
forefront
microbial
genomics.
From
participants’
experiences,
aimed
to
identify
barriers
facilitators
driving
development
implementation
metagenome
as
a
diagnostic.
Methods
Virtual
semi-structured
interviews
were
conducted
with
purposively
selected
individuals
involved
The
implementing
diagnostic
tool
decisions
about
which
used
identifying
bacteria-causing
Thematic
analysis
was
analyse
data,
an
Interpretive
Phenomenological
approach
throughout.
Results
Ten
interviewed
between
July
2021
October
2021,
scientists,
consultants,
professors
academia.
Their
experience
ranged
from
limited
knowledge
expert
understanding
phenomenon.
thoughts
perspectives
participants
could
be
grouped
five
themes:
Availability
diseases;
Clinical
laboratory
infrastructure;
Ethical
Data
Sharing:
Enhancing
metagenomics
through
Open
Access;
Case
action:
COVID-19;
importance
communication
improve
developments
new
diagnostics.
Participants
recognised
need
implemented
overcome
limitations
current
approaches
but
highlighted
integrating
settings,
such
impact
on
decision-making,
accreditation,
cost.
Further,
felt
that
lessons
learnt
using
COVID-19
how
other
platforms
have
integrated
over
last
20
years.
Conclusions
provided
insights
opinions
address
gap
literature
identified
drive
IDD
settings.
Knowledge
upcoming
genomic
testing
is
not
equally
distributed
throughout
UK,
impacting
integrate
Improvements
access
positively
population
health.
Язык: Английский
Clinical impact of bronchoalveolar lavage fluid metagenomic next-generation sequencing in immunocompromised patients with severe community-acquired pneumonia in ICU: a multicenter retrospective study
Infection,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 23, 2025
An
increasing
number
of
critically
ill
patients
are
immunocompromised.
These
at
high
risk
intensive
care
unit
(ICU)
admission
because
numerous
complications.
Acute
respiratory
failure
due
to
severe
community-acquired
pneumonia
(SCAP)
is
one
the
leading
causes
admission.
Early
targeted
antibiotic
therapy
crucial
for
improving
prognosis
these
patients.
Metagenomic
next-generation
sequencing
(mNGS)
in
bronchoalveolar
lavage
fluid
(BALF)
has
shown
significant
value
pathogen
detection
recent
years.
However,
there
few
studies
on
summarizing
profiles
SCAP
immunocompromised
We
performed
a
multicenter
retrospective
analysis
with
ICU
diagnosed
between
May
2021
October
2024.
Bronchoalveolar
(BALF),
blood,
and
sputum
samples
were
collected
subjected
mNGS
conventional
microbiological
tests
(CMTs).
The
detected
by
two
methods
compared.
In
our
study,
compared
CMTs,
increased
rates
mixed
infections
group
(58.82%
vs
17.96%,
P
<
0.05)
immunocompetent
(44.58%
18.72%,
0.05),
while
also
reducing
rate
no
(4.90%
38.73%,
0.05;
8.37%
32.76%,
0.05).
both
groups,
proportion
positive
clinical
impacts
(diagnosis)
resulting
from
results
exceeded
90%
(96.57%
93.84%),
treatment
effectiveness
was
higher
than
(65.69%
56.40%,
Further
showed
that
when
mNGS-guided
effective,
28-day
mortality
significantly
improved
(31.34%
74.29%,
(42.36%
40.68%,
ineffective.
This
study
indicates
SCAP,
particularly
those
who
immunocompromised,
more
likely
have
polymicrobial
infections.
BALF
provides
rapid
comprehensive
profiling
pulmonary
infections,
thereby
having
impact
diagnosis,
SCAP.
Язык: Английский
What’s new in the management of pulmonary complications in allogeneic stem cell transplantation?
Expert Review of Respiratory Medicine,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 29, 2025
As
survival
increases
after
allogeneic
hematopoietic
stem
cell
transplantation
(allo-HCT),
several
organ
complications
have
emerged,
including
those
involving
the
lung,
which
require
a
multidisciplinary
management
approach.
The
constant
evolution
of
allo-HCT
procedures,
advances
in
diagnostic
tools
for
infections
and
pulmonary
disease,
as
well
new
treatment
approaches,
frequent
updating
knowledge
this
field.
We
review
multiple
infectious
noninfectious
lung
that
occur
both
early
late
allo-HCT.
This
includes
an
updated
description
these
complications,
risk
factors,
approach
outcome.
A
literature
search
was
performed
using
PubMed-indexed
journals.
diagnosis
remains
challenging,
further
complicated
by
association
co-infections
and/or
links
between
infection
complications.
development
metagenomic
next-generation
sequencing
(mNGS)
should
enhance
yield
bronchoalveolar
lavage
but
its
clinical
relevance
to
be
evaluated.
better
understanding
pathophysiology
chronic
graft-versus-host
disease
(GVHD)
improved
phenotyping
are
essential
advancing
therapeutic
management.
requires
revision
criteria
identification
biomarkers
disease.
Язык: Английский
Clinical relevance of lung microbiota composition in critically ill children with acute lower respiratory tract infections: insights from a retrospective analysis of metagenomic sequencing
Zhiyu Men,
Zhiheng Chen,
Xiyao Gu
и другие.
European Journal of Clinical Microbiology & Infectious Diseases,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 9, 2024
Acute
lower
respiratory
tract
infections
(ALRIs)
is
a
leading
cause
of
child
mortality
worldwide.
Metagenomic
next-generation
sequencing
(mNGS)
identifies
ALRIs
pathogens
and
explores
the
lung
microbiota's
role
in
disease
severity
clinical
outcomes.
This
study
examines
association
between
microbiota
outcomes
children,
exploring
its
potential
as
prognostic
biomarker.
Язык: Английский
Pulmonary infection caused by Tropheryma whipplei: a case report and review of the literature
Journal of Medical Case Reports,
Год журнала:
2024,
Номер
18(1)
Опубликована: Дек. 28, 2024
Abstract
Background
Tropheryma
whipplei
pneumonia
is
an
infrequent
medical
condition.
The
clinical
symptoms
associated
with
this
disease
are
nonspecific,
often
resulting
in
misdiagnosis
or
missed
diagnosis.
Therefore,
sharing
and
summarizing
the
experiences
diagnosis
treatment
of
can
deepen
global
understanding
awareness
it.
Case
presentation
patient
a
78-year-old
married
Han
Chinese
female
who
was
admitted
to
hospital
after
experiencing
fever,
dry
cough,
fatigue
for
4
days.
A
lung
computed
tomography
scan
revealed
inflammatory
exudation
lower
left
lung,
accompanied
by
pleural
effusion.
bronchoalveolar
lavage
fluid
subjected
further
analysis
using
metagenomic
next-generation
sequencing,
which
identified
41
genetic
sequences
.
Consequently,
she
diagnosed
pneumonia.
After
initiating
doxycycline
biapenem,
patient’s
showed
significant
improvement.
Upon
discharge,
continued
combination
hydroxychloroquine,
discontinued
At
12-month
follow-up,
reported
overall
good
health,
no
any
other
discomfort.
Conclusion
rare
condition
nonspecific
symptoms.
application
sequencing
technology
pulmonary
infections
helps
rapidly
identify
pathogens,
providing
solid
foundation
precise
effective
antibacterial
patients.
Язык: Английский