Application and Progression of Single‐Cell RNA Sequencing in Diabetes Mellitus and Diabetes Complications
Jiajing Hong,
Shiqi Lu,
Guohui Shan
и другие.
Journal of Diabetes Research,
Год журнала:
2025,
Номер
2025(1)
Опубликована: Янв. 1, 2025
Diabetes
is
a
systemic
metabolic
disorder
primarily
caused
by
insulin
deficiency
and
resistance,
leading
to
chronic
hyperglycemia.
Prolonged
diabetes
can
result
in
damage
multiple
organs,
including
the
heart,
brain,
liver,
muscles,
adipose
tissue,
thereby
causing
various
fatal
complications
such
as
diabetic
retinopathy,
cardiomyopathy,
nephropathy.
Single‐cell
RNA
sequencing
(scRNA‐seq)
has
emerged
valuable
tool
for
investigating
cell
diversity
pathogenesis
of
identifying
potential
therapeutic
targets
or
complications.
This
review
provides
comprehensive
overview
recent
applications
scRNA‐seq
diabetes‐related
researches
highlights
novel
biomarkers
immunotherapy
with
cell‐type
information
its
associated
Язык: Английский
Advances of research in diabetic cardiomyopathy: diagnosis and the emerging application of sequencing
Frontiers in Cardiovascular Medicine,
Год журнала:
2025,
Номер
11
Опубликована: Янв. 13, 2025
Diabetic
cardiomyopathy
(DCM)
is
one
of
the
most
prevalent
and
severe
complications
associated
with
diabetes
mellitus
(DM).
The
onset
DCM
insidious,
symptoms
being
obvious
only
in
late
stage.
Consequently,
early
diagnosis
a
formidable
challenge
which
significantly
influences
treatment
prognosis
DCM.
Thus,
it
becomes
imperative
to
uncover
innovative
approaches
facilitate
prompt
identification
On
traditional
clinical
side,
we
tend
use
serum
biomarkers
as
well
imaging
common
means
diagnosing
diseases
because
their
convenience
affordability.
As
delve
deeper
into
mechanisms
DCM,
wide
variety
are
becoming
competitive
diagnostic
indicators.
Meanwhile,
application
multiple
techniques
has
also
made
efforts
promote
Besides,
spurt
sequencing
technology
possible
give
hints
about
disease
from
genome
transcriptome,
making
less
difficult,
more
sensitive,
predictive.
Overall,
expected
be
superior
choice
plasma
for
detecting
lesions
at
an
earlier
stage
than
imaging,
its
judicious
utilization
combined
technologies
will
lead
sensitive
future.
Therefore,
this
review
meticulously
consolidates
progress
various
biomarkers,
methods,
realm
diagnosis,
aim
furnishing
novel
theoretical
foundation
guide
future
research
endeavors
towards
enhancing
therapeutic
landscape
Язык: Английский
Genome-wide chromatin accessibility and selective signals of meat rabbits reveal key Cis-regulatory elements and variants during postnatal development of skeletal muscles in rabbits
BMC Genomics,
Год журнала:
2025,
Номер
26(1)
Опубликована: Март 25, 2025
The
development
of
skeletal
muscles
is
intricately
modulated
by
multiple
genetic
factors
and
significantly
impacts
the
economic
value
meat
rabbits.
However,
our
knowledge
epigenetics
in
rabbit
remains
largely
unknown.
In
this
study,
we
collected
leg
rabbits
performed
assays
for
transposase-accessible
chromatin
with
high
throughput
sequencing
(ATAC-seq)
to
detect
open
across
three
developmental
stages:
birth
(D1),
weaning
(D35),
adulthood
(D75).
A
total
126,959
accessible
regions
(ACRs)
were
identified
samples,
a
broad
increase
decrease
accessibility
found
from
D1
D35
D75,
respectively.
Integrative
analysis
transcriptome
data
revealed
ACRs
that
nearly
closed
at
but
highly
D75
enriched
muscle
development.
Cis-regulation
further
genes
dominated
enhancers
mainly
play
roles
neuron
muscles.
Moreover,
detection
selection
signals
footprinting
transcription
factor
both
base
transversion
(Chr13:12144967
A->
G)
dynamics
PRDM1
binding
site
might
regulate
ZSWIM5
during
Our
study
provided
category
potential
cis-regulatory
elements
understanding
tissue
level
facilitate
insights
into
growth
regulation
Язык: Английский
Cardiac PDK4 promotes neutrophilic PFKL methylation and drives the innate immune response in diabetic myocardial infarction
Pharmacological Research,
Год журнала:
2025,
Номер
unknown, С. 107731 - 107731
Опубликована: Апрель 1, 2025
Язык: Английский
Knockout of B2M in combination with PD-L1 overexpression protects MSC-derived new islet β cells from graft rejection in the treatment of canine diabetes mellitus
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Дек. 2, 2024
The
immunogenicity
of
allogeneic
mesenchymal
stem
cells
(MSCs)
is
significantly
enhanced
after
transplantation
or
differentiation,
and
these
can
be
recognized
cleared
by
recipient
immune
cells.
Graft
rejection
has
become
a
major
obstacle
to
improving
the
therapeutic
effect
MSCs
or,
their
in
treatment
diabetes
other
diseases.
Solving
this
problem
helpful
for
prolonging
time
that
play
role
body
clinical
effect.
In
study,
canine
adipose-derived
(ADSCs)
were
used
as
seed
cells,
gene
editing
technology
was
knock
out
B2M
cooperate
with
overexpression
PD-L1
gene.
Gene-edited
ADSCs
(GeADSCs),
whose
biological
characteristics
safety
are
not
different
from
those
normal
ADSCs,
have
been
obtained.
GeADSCs
reduced,
escape
ability
enhanced,
remain
longer
time.
Using
optimized
induction
program,
efficiency
differentiation
into
new
islet
β-cells
increased,
maturity
increased.
decreased,
transplanted
diabetic
dogs
(the
graft
site
prevascularized
implantation
scaffold
form
vascularized
pouch).
number
infiltrating
content
factors
decreased
at
site.
New
β-cell
transplantation,
which
low
immunogenicity,
reverse
dogs,
cell
enhanced.
This
study
provides
method
survival
functional
transplant
recipients
Язык: Английский
Inhibition of Atrial Natriuretic Peptide Clearance Reduces Myocardial Fibrosis and Improves Cardiac Function in Diabetic Rats
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 3, 2024
Abstract
Background
Natriuretic
peptides
(NP)
exert
pleotropic
effects
through
the
recruitment
of
cGMP-signaling
pathways
depending
on
their
bioavailability
which
is
regulated
by
clearance
receptors
and
peptidases.
Here,
we
tested
hypothesis
that
increasing
myocardial
NP
has
a
beneficial
effect
heart
failure.
We
studied
mutated
NP,
MANP,
resistant
to
neprilysin
in
model
diabetic
cardiomyopathy
characterized
marked
fibrosis.
Methods
as
well
sacubritril
were
delivered
via
osmotic
mini-pumps
high-fat/streptozotocin-induced
type-2
(T2D)
rats.
Cardiac
function
was
evaluated
echocardiography.
Myocardial
remodeling
histological
approaches,
collagen
phenotype
measurement
cGMP
tissue
concentration.
Live-cell
biosensing
conducted
cultured
rat
cardiac
fibroblasts
investigate
biological
NPs.
signaling
pathway
using
various
antibody
arrays
biochemicals
assays
fibroblasts.
Results
MANP
exhibits
superior
efficacy
than
ANP
reducing
left
ventricular
dysfunction
reduce
fibrosis
with
less
extracellular
matrix
deposition.
In
vitro
,
similarly
generated
activated
PKG
fibroblasts,
attenuating
SMAD
activation,
secretion
cell
proliferation.
Nevertheless,
vivo
enhanced
accumulation
more
potent
activating
cGMP/PKG
inhibiting
profibrotic
pathway.
Endopeptidase
inhibition
sacubitril
also
led
reduced
Conclusions
major
determinant
peptide
improving
pump
during
cardiomyopathy.
Clinical
Perspective
What
Is
New?
Mutated
atrial
natriuretic
(MANP)
degradation
outperforms
wild-type
diabetes
While
antifibrotic
two
isoforms
involves
cGMP-dependent
fibroblast
concentration
importantly
ANP.
Sacubitril
inhibits
reduces
activation
function.
Are
Implications?
crucial
for
mitigating
failure
general.
holds
potential
new
treatment
modality
management
Язык: Английский