Frontiers in Endocrinology, Год журнала: 2025, Номер 16

Опубликована: Апрель 17, 2025

Backgrounds Diabetic cardiomyopathy has a very high incidence and serious clinical consequences, making it an urgent problem to be solved. Angiogenesis is significant phenotype in the occurrence development of diabetic cardiomyopathy, especially damage angiogenesis cardiac microvessels, which inextricably linked risk patients. In current basic research, there still lack treatment methods that directly target cardiomyopathy. This study hopes discover key molecules related damage, provide ideas for possible interventions. Methods Sequencing data animals cells were obtained from GEO database, differentially expressed genes analyzed. Subsequently, angiogenesis-related clustered functional pathway analysis. Then, microangiogenesis mice changes glucose-stimulated HUVECs verified, top three verified using western blot. Results 24 associated with found GSE241565(human) GSE215979(mice). Among them, 11 showed same trend two databases. Then CD31 staining hearts microvascular was impaired, decreased tube formation, wound healing migration weakened. Finally, 3 most no difference between Edn1 Lepr. At time, Efnb2 significantly increased under glucose stimulation. Conclusion Combined sequencing animal cell models differential screened. These findings not only elucidate novel molecular axis linking but also highlight as potential therapeutic target.

Язык: Английский