Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods DOI Creative Commons
Linlin Zhuang, Ying Zhao,

Jingyi Shen

и другие.

Discover Nano, Год журнала: 2025, Номер 20(1)

Опубликована: Март 3, 2025

Porcine epidemic diarrhea (PED) is a highly contagious intestinal disease caused by the porcine virus (PEDV). The economic impact of PEDV on global pig industry has been significant, resulting in considerable losses. This paper presents review latest research progress genome, molecular epidemiology, vaccine development, and detection methods. It was determined that genetic diversity spike (S) gene closely associated with epidemiological trend PEDV. prevalence S variants different genotypes exhibited variability across regions populations. Epidemiological analyses have demonstrated can be transmitted via multiple routes, including direct contact, airborne aerosol, water source contamination. With regard to research, available vaccines classified into several categories, live-attenuated vaccines, inactivated subunit bacterial vector viral mRNA etc. Each these distinctive characteristics terms immunogenicity, protection efficiency, safety. Molecular methods, PCR-based isothermal amplification techniques, immunological assays, biosensors, play an important role diagnosis monitoring Furthermore, this examines current developments identifies key areas future investigation. objective establish theoretical foundation for prevention control strategies PED, provide point reference further genomics, development methods

Язык: Английский

Molecular characterization of porcine epidemic diarrhea virus in Sichuan from 2023 to 2024 DOI

Bo Xie,

Wenjun Yan, Xin Yang

и другие.

Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107486 - 107486

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

2
Evaluation of an F Protein-Based Recombinant Protein for Immunization Against Respiratory Syncytial Virus DOI
Alicia Hernández-Mercado, Claudia Berenice Barrón-García,

Jayline Romo-Amador

и другие.

Viral Immunology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 10, 2025

Respiratory syncytial virus (RSV) is one of the most important etiologies acute respiratory infections that cause bronchiolitis in children under 5 years age. Treatments are expensive, no vaccine available, and this an hospitalization. Costimulatory molecules have been reported to be good inducers antiviral type 1 immune response. This study aimed generate a recombinant with RSV F protein fused 4-1BBL evaluate activation response vitro production neutralizing antibodies vivo. The codon-optimized gene was subcloned into expression vector as follows: streptavidin core, F, costimulatorytumor necrosis factor receptor superfamily member 9 -TNFRS9- ligand (4-1BBL). After induction Escherichia coli C43, (SA-F3x-4-1BBL, denominated SF4) purified verified by western blotting. Cultured RAW264.7 macrophages were stimulated SF4 protein, then tumor (TNF) receptor-associated 2 (TRAF2), p38, nuclear κ-light-chain-enhancer activated B cells (NF-κB) analyzed blot, mRNA cytokines RT-qPCR. Finally, male C57BL/6 mice inoculated SF4, generation anti-RSV serum examined. had size 84.4 kDa 5.6% yield. SA-F-4-1BBL upregulated TRAF2, TNF-α, interferon (IFN)-γ levels p38 mitogen-activated kinase NF−κΒ pathways cells. Importantly, capable infection producing detected sera immunized animals. These results suggest fusion activates signaling pathway, resulting effective mediated cytokines.

Язык: Английский

Процитировано

1
Towards developing multistrain PEDV vaccines: Integrating basic concepts and SARS-CoV-2 pan-sarbecovirus strategies DOI Creative Commons
Mario Fragoso-Saavedra, Qiang Liu

Virology, Год журнала: 2025, Номер 604, С. 110412 - 110412

Опубликована: Янв. 19, 2025

Язык: Английский

Процитировано

0
Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods DOI Creative Commons
Linlin Zhuang, Ying Zhao,

Jingyi Shen

и другие.

Discover Nano, Год журнала: 2025, Номер 20(1)

Опубликована: Март 3, 2025

Porcine epidemic diarrhea (PED) is a highly contagious intestinal disease caused by the porcine virus (PEDV). The economic impact of PEDV on global pig industry has been significant, resulting in considerable losses. This paper presents review latest research progress genome, molecular epidemiology, vaccine development, and detection methods. It was determined that genetic diversity spike (S) gene closely associated with epidemiological trend PEDV. prevalence S variants different genotypes exhibited variability across regions populations. Epidemiological analyses have demonstrated can be transmitted via multiple routes, including direct contact, airborne aerosol, water source contamination. With regard to research, available vaccines classified into several categories, live-attenuated vaccines, inactivated subunit bacterial vector viral mRNA etc. Each these distinctive characteristics terms immunogenicity, protection efficiency, safety. Molecular methods, PCR-based isothermal amplification techniques, immunological assays, biosensors, play an important role diagnosis monitoring Furthermore, this examines current developments identifies key areas future investigation. objective establish theoretical foundation for prevention control strategies PED, provide point reference further genomics, development methods

Язык: Английский

Процитировано

0