Molecular characterization of porcine epidemic diarrhea virus in Sichuan from 2023 to 2024
Microbial Pathogenesis,
Год журнала:
2025,
Номер
unknown, С. 107486 - 107486
Опубликована: Март 1, 2025
Язык: Английский
Evaluation of an F Protein-Based Recombinant Protein for Immunization Against Respiratory Syncytial Virus
Viral Immunology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 10, 2025
Respiratory
syncytial
virus
(RSV)
is
one
of
the
most
important
etiologies
acute
respiratory
infections
that
cause
bronchiolitis
in
children
under
5
years
age.
Treatments
are
expensive,
no
vaccine
available,
and
this
an
hospitalization.
Costimulatory
molecules
have
been
reported
to
be
good
inducers
antiviral
type
1
immune
response.
This
study
aimed
generate
a
recombinant
with
RSV
F
protein
fused
4-1BBL
evaluate
activation
response
vitro
production
neutralizing
antibodies
vivo.
The
codon-optimized
gene
was
subcloned
into
expression
vector
as
follows:
streptavidin
core,
F,
costimulatorytumor
necrosis
factor
receptor
superfamily
member
9
-TNFRS9-
ligand
(4-1BBL).
After
induction
Escherichia
coli
C43,
(SA-F3x-4-1BBL,
denominated
SF4)
purified
verified
by
western
blotting.
Cultured
RAW264.7
macrophages
were
stimulated
SF4
protein,
then
tumor
(TNF)
receptor-associated
2
(TRAF2),
p38,
nuclear
κ-light-chain-enhancer
activated
B
cells
(NF-κB)
analyzed
blot,
mRNA
cytokines
RT-qPCR.
Finally,
male
C57BL/6
mice
inoculated
SF4,
generation
anti-RSV
serum
examined.
had
size
84.4
kDa
5.6%
yield.
SA-F-4-1BBL
upregulated
TRAF2,
TNF-α,
interferon
(IFN)-γ
levels
p38
mitogen-activated
kinase
NF−κΒ
pathways
cells.
Importantly,
capable
infection
producing
detected
sera
immunized
animals.
These
results
suggest
fusion
activates
signaling
pathway,
resulting
effective
mediated
cytokines.
Язык: Английский
Towards developing multistrain PEDV vaccines: Integrating basic concepts and SARS-CoV-2 pan-sarbecovirus strategies
Virology,
Год журнала:
2025,
Номер
604, С. 110412 - 110412
Опубликована: Янв. 19, 2025
Язык: Английский
Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods
Discover Nano,
Год журнала:
2025,
Номер
20(1)
Опубликована: Март 3, 2025
Porcine
epidemic
diarrhea
(PED)
is
a
highly
contagious
intestinal
disease
caused
by
the
porcine
virus
(PEDV).
The
economic
impact
of
PEDV
on
global
pig
industry
has
been
significant,
resulting
in
considerable
losses.
This
paper
presents
review
latest
research
progress
genome,
molecular
epidemiology,
vaccine
development,
and
detection
methods.
It
was
determined
that
genetic
diversity
spike
(S)
gene
closely
associated
with
epidemiological
trend
PEDV.
prevalence
S
variants
different
genotypes
exhibited
variability
across
regions
populations.
Epidemiological
analyses
have
demonstrated
can
be
transmitted
via
multiple
routes,
including
direct
contact,
airborne
aerosol,
water
source
contamination.
With
regard
to
research,
available
vaccines
classified
into
several
categories,
live-attenuated
vaccines,
inactivated
subunit
bacterial
vector
viral
mRNA
etc.
Each
these
distinctive
characteristics
terms
immunogenicity,
protection
efficiency,
safety.
Molecular
methods,
PCR-based
isothermal
amplification
techniques,
immunological
assays,
biosensors,
play
an
important
role
diagnosis
monitoring
Furthermore,
this
examines
current
developments
identifies
key
areas
future
investigation.
objective
establish
theoretical
foundation
for
prevention
control
strategies
PED,
provide
point
reference
further
genomics,
development
methods
Язык: Английский