Mixed Cultures for Biodegradation of Lindane: From Genome‐Based Assembly to Practical Assessment

Remediation Journal, Год журнала: 2025, Номер 35(2)

Опубликована: Янв. 1, 2025

ABSTRACT Lindane, or γ‐hexachlorocyclohexane (γ‐HCH), was one of the most widely used organic pesticides in 20th century, but has been banned worldwide due to its high toxicity and harmful effects humans animals. However, because resistance decomposition, lindane can be persistent (with a long half‐life up several years natural environment) thus bioaccumulate through food web, thereby causing serious contamination. Currently, addition physical–chemical methods, innovative biodegradation‐based methods for treating contamination are preferred their sustainability effectiveness. In this study, we explored possibility constructing mixed microbial cultures that could provide improved degradation efficiencies, based on genome annotation, using KEGG database subsequent predictions metabolic interactions between single strains. Based such genome‐mining results, proposed 10 potential lindane‐degrading different strain combinations evaluated performance liquid, semi‐solid, solid media. The results showed mixtures displayed higher efficiencies versus cultures, by 20%–30%, liquid medium, although performances were not remarkable semi‐solid well‐performing also adapted broader spectra environmental conditions. We hypothesized central roles two core strains ( Pseudomonas putida PAM66 Streptommyces katrae A119) complicated among component might attributed mixtures. Overall, our study supports use constructed improving biodegradation, especially xenobiotics like lindane, emphasizes importance exploiting techniques design those

Язык: Английский

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Studies on the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in aerobic granular sludge from a WWTP: adaptability of native prokaryotes and enhancement of paracetamol intermediate metabolites biodegradation

Environmental Technology & Innovation, Год журнала: 2025, Номер 38, С. 104073 - 104073

Опубликована: Фев. 6, 2025

Язык: Английский

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BIOREMEDIATION: A SUPERIOR ALTERNATIVE FOR REMEDIATING TANNERY EFFLUENT-CONTAMINATED SOIL

FUDMA Journal of Sciences, Год журнала: 2025, Номер 9(2), С. 193 - 208

Опубликована: Фев. 28, 2025

Tannery effluent poses significant risks to soil health, primarily through contamination with heavy metals like chromium, sulphides, and persistent organic pollutants (POPs). These toxic substances inhibit microbial activity, reducing nutrient cycling matter decomposition essential for fertility. Beneficial microorganisms, including nitrogen-fixing bacteria, are particularly affected, leading altered communities dominated by less advantageous, metal-tolerant species. Accumulation of POPs disrupts enzymatic activities, interferes plant root growth, complicates remediation efforts due pollutant migration groundwater potential entry into the food chain. Prolonged exposure such contaminants diminishes fertility, reduces resilience, ecosystem services, posing threats agricultural productivity environmental health. This review was aimed outline what made bioremediation a superior treatment technology among other methods used in remediating tannery contaminated soil. Efforts mitigate impacts involve combination physical, chemical, biological technologies. Physical washing, flushing, thermal desorption focus on removing or isolating contaminants, while chemical approaches as oxidation, reduction, stabilization transform harmful forms immobilize them. Biological leverages microorganisms plants detoxify sustainably. Bioremediation strategies aid bioaugmentation biostimulation do enhance activity address inorganic effectively more than physical methods. Another excellent called phytoremediation can also effectively, Achieving better technique should be coupled stringent industrial regulations, sustainable tanning methods, stakeholder awareness

Язык: Английский

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Metagenome Analysis Identified Novel Microbial Diversity of Sandy Soils Surrounded by Natural Lakes and Artificial Water Points in King Salman Bin Abdulaziz Royal Natural Reserve, Saudi Arabia

Life, Год журнала: 2024, Номер 14(12), С. 1692 - 1692

Опубликована: Дек. 20, 2024

Background: Soil microbes play a vital role in the ecosystem as they are able to carry out number of tasks. Additionally, metagenomic studies offer valuable insights into composition and functional potential soil microbial communities. Furthermore, analyzing obtained data can improve agricultural restoration practices aid developing more effective environmental management strategies. Methodology: In November 2023, sandy samples were collected from ten sites different geographical areas surrounding natural lakes artificial water points Tubaiq conservation area King Salman Bin Abdulaziz Royal Natural Reserve (KSRNR), Saudi Arabia. addition, genomic DNA was extracted samples, 16S rRNA sequencing conducted using high-throughput Illumina technology. Several computational analysis tools used for gene prediction taxonomic classification groups. Results: this study, surroundings resources two distinct natures used. Based on sequencing, total 24,563 OTUs detected. The information then categorized 446 orders, 1036 families, 4102 genera, 213 classes, 181 phyla. Moreover, phylum Pseudomonadota most dominant community across all representing an average relative abundance 34%. Actinomycetes abundant class (26%). clustered proteins assigned COG categories provides detailed understanding capabilities adaptation communities samples. Amino acid metabolism transport environment. Conclusions: Metagenome soils KSRNR (Saudi Arabia) has unveils rich activity, highlighting complex interactions ecological roles these environments.

Язык: Английский

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