Elsevier eBooks, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Military Medical Research, Год журнала: 2025, Номер 12(1)
Опубликована: Фев. 11, 2025
Abstract Cancer recurrence, driven by the phenomenon of tumor dormancy, presents a formidable challenge in oncology. Dormant cancer cells have ability to evade detection and treatment, leading relapse. This review emphasizes urgent need comprehend dormancy its implications for recurrence. Despite notable advancements, significant gaps remain our understanding mechanisms underlying lack reliable biomarkers predicting provides comprehensive analysis cellular, angiogenic, immunological aspects dormancy. It highlights current therapeutic strategies targeting dormant cells, particularly combination therapies immunotherapies, which hold promise preventing By elucidating these proposing innovative research methodologies, this aims deepen ultimately facilitating development more effective recurrence improving patient outcomes.
Язык: Английский
Процитировано
1
Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 175, С. 116704 - 116704
Опубликована: Май 14, 2024
Long non-coding RNAs (lncRNAs) are pivotal controllers of gene expression through epigenetic mechanisms, Methylation, a prominent area study in epigenetics, significantly impacts cellular processes. Various RNA base methylations, including m6A, m5C, m1A, and 2'-O-methylation, profoundly influence lncRNA folding, interactions, stability, thereby shaping their functionality. LncRNAs methylation contribute to tumor development, especially lung cancer. Their roles encompass cell differentiation, proliferation, the generation cancer stem cells, modulation immune responses. Recent studies have suggested that dysregulation can development. Furthermore, modifications lncRNAs hold potential for clinical application Dysregulated promote progression may offer insights into biomarker or therapeutic target. This review summarizes current knowledge its implications epigenetics pulmonary diseases.
Язык: Английский
Процитировано
6
Frontiers in Oncology, Год журнала: 2025, Номер 14
Опубликована: Янв. 7, 2025
Cancer's epigenetic landscape, a labyrinthine tapestry of molecular modifications, has long captivated researchers with its profound influence on gene expression and cellular fate. This review discusses the intricate mechanisms underlying cancer epigenetics, unraveling complex interplay between DNA methylation, histone chromatin remodeling, non-coding RNAs. We navigate through tumultuous seas dysregulation, exploring how these processes conspire to silence tumor suppressors unleash oncogenic potential. The narrative pivots cutting-edge technologies, revolutionizing our ability decode epigenome. From granular insights single-cell epigenomics holistic view offered by multi-omics approaches, we examine tools are reshaping understanding heterogeneity evolution. also highlights emerging techniques, such as spatial long-read sequencing, which promise unveil hidden dimensions regulation. Finally, probed transformative potential CRISPR-based epigenome editing computational analysis transmute raw data into biological insights. study seeks synthesize comprehensive yet nuanced contemporary landscape future directions research.
Язык: Английский
Процитировано
0
Computers in Biology and Medicine, Год журнала: 2025, Номер 188, С. 109811 - 109811
Опубликована: Фев. 17, 2025
Gliomas are highly heterogeneous tumors with generally poor prognoses. Leveraging multi-omics data and network analysis holds great promise in uncovering crucial signatures molecular relationships that elucidate glioma heterogeneity. However, the complexity of problem high dimensionality increase challenges integrating information across various biological levels. This study develops a comprehensive framework aimed at identifying potential glioma-type-specific biomarkers through innovative variable selection integrated visualization. We designed two-step for using sparse estimation omics datasets. incorporates MINGLE (Multi-omics Integrated Network GraphicaL Exploration), novel methodology to merge distinct into single network, enabling identification underlying relations an The was conducted datasets, patients grouped based on latest classification guidelines. Our investigation led variables potentially serving as biomarkers. integration facilitated discovery reflect heterogeneity, supporting interpretation. Scripts files reproducing or adapting it other applications, available R software.
Язык: Английский
Процитировано
0
Clinical Epigenetics, Год журнала: 2025, Номер 17(1)
Опубликована: Фев. 17, 2025
Homeobox A9 promoter methylation (HOXA9) has been reported as a biomarker for early lung adenocarcinoma patients' prognosis. We aim to evaluate its prognostic value, regardless of disease stage. Using droplet digital PCR, we measured HOXA9 in cohort comprising 161 Brazilian patients. Low was associated with higher cancer-specific survival but showed no significance after adjustment clinical covariates. While low earlier stages, association observed this subset Overall, is not an independent adenocarcinomas
Язык: Английский
Процитировано
0
Brain Pathology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 28, 2025
DNA methylation profiling by Illumina array-based methods has revolutionized the molecular classification and diagnosis of brain tumors. A significant barrier to adopting these in a clinical environment is requirement for specialized scanners, which results high additional costs larger laboratory footprint. sequencing-based alternatives are attractive because most pathology laboratories already use sequencers other assays. This study aimed compare utility newly developed enzymatic methyl sequencing (EM-seq) method paired with Twist Human Methylome panel tumor standard Infinium Methylation BeadChip-based methods. We used from fresh-frozen or formalin-fixed, paraffin-embedded (FFPE) cancer samples 19 patients 1 control sample construct libraries covering 3.98 million CpG sites. validated bioinformatics pipeline analyze target-enriched EM-seq (TEEM-seq) data comparison copy number profiling. found concordance between TEEM-seq traditional methods, correlation coefficients (>0.98) FFPE replicates. successfully classified into expected classes robust prediction scores (>0.82). observed that required depth at least 35x achieve consistently reliable scores. The potential complement existing lower barriers adoption routine use.
Язык: Английский
Процитировано
0
PubMed, Год журнала: 2025, Номер 14(2), С. 149 - 156
Опубликована: Янв. 1, 2025
Acute myeloid leukemia (AML) is the most frequent type of among adults. Investigating AML heterogeneity based on DNA methylation can improve clinical diagnosis and prognosis. This study was conducted to investigate NR4A1 NR4A3 gene in fifty newly diagnosed patients healthy controls using Methyl specific PCR (MSP). The frequency rs1569686 DNMT3B also determined by Tetra primer ARMS PCR. Also, association between studied genes some prognostic marker including mutation FLT3 NPM genes, as well hematological factors evaluated. According findings, have a significantly higher prevalence methylated than those without AML. with un-methylated had FLT-ITD positivity NR4A3. there no significant Finally, distribution different genotypes did not show any association. results found that were hyper-methylated patients. However, polymorphism main contributor status gene. Future studies should consider other mechanisms influencing role hypermethylation
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142401 - 142401
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(4), С. 227 - 227
Опубликована: Март 26, 2025
Myeloproliferative neoplasms are clonal hematological characterized by excessive proliferation of cells erythroid, granulocytic, and megakaryocytic lineage. The genetic mechanisms underlying this group blood diseases now known, but new perspectives have recently emerged in the field epigenetics particularly related to possible role DNA methylation disease development progression. regulates different cellular processes, such as proliferation, differentiation, apoptosis. In myeloproliferative neoplasms, a link has been found between abnormal patterns, hypermethylation tumor suppressors or, conversely, oncogenes hypomethylation, with progression disease, spreading important prognostic therapeutic implications. This review aims investigate relationship alterations emphasizing ways which epigenetic dysregulation promotes biology.
Язык: Английский
Процитировано
0
The Nucleus, Год журнала: 2025, Номер unknown
Опубликована: Апрель 8, 2025
Язык: Английский
Процитировано
0