The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysm DOI Creative Commons
Xuanxin Lyu,

Ming Jiang,

Jiahao Shi

и другие.

PeerJ, Год журнала: 2025, Номер 13, С. e19222 - e19222

Опубликована: Апрель 7, 2025

Background In recent years, a novel animal abdominal aortic aneurysm (AAA) model was established by administering erythropoietin (EPO) to wild-type (WT) mice. However, the influence of EPO on murine fecal microbiota remains uninvestigated. Therefore, this study aims explore potential association between gut changes and AAA development in model. Methods results Adult male C57BL/6 mice were used establish intraperitoneal administration recombinant human at dosage 10,000 IU/kg daily for 28 consecutive days. Hematoxylin eosin (H&E) Elastin Van Gieson (EVG) staining revealed that increased wall thickness diameter, accompanied enhanced degradation elastic lamina. The 16S rRNA—sequencing data deposited Sequence Read Archive (PRJNA1172300). LEfSe analysis Akkermansia, Lawsonibacter, Clostridium, Neglectibacter significantly associated with EPO-induced development, while Lactobacillus, Alistipes, Limosilactobacillus, Eisenbergiella showed significant negative correlations. Analysis using Kyoto Encyclopedia Genes Genomes (KEGG) prediction module differences metabolic pathways two groups, including alanine, aspartate glutamate metabolism; cysteine methionine pyrimidine carbon ABC transporters; oxidative phosphorylation pathways. Conclusions dysbiosis, particularly Alistipes abundance, may contribute formation via inflammation, stress, dysfunction. While advances research, its limitations underscore need validation mechanistic studies. Future work should prioritize multi-omics integration cross-model comparisons unravel complex microbiota-AAA axis.

Язык: Английский

Deciphering the association between gut microbiome and varicose veins: A mendelian randomization study DOI
Yu Tian, Furong Hao, Jiantao Zhang

и другие.

Phlebology The Journal of Venous Disease, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

Background: Despite the well-documented influence of traditional risk factors on varicose veins, potential causal role gut microbiome in this condition remains underexplored. This study aims to elucidate association between specific microbial taxa and veins using a Mendelian Randomization (MR) approach. Methods: Utilizing genome-wide datasets from MiBioGen Consortium Finnish database, carefully selected instrumental variables based their genetic with stringent statistical criteria. Multiple MR techniques were applied analyze data, accompanied by comprehensive sensitivity analyses ensure reliability relationships microbiota veins. Results: The identified three taxa—genus Terrisporobacter , Coprococcus2 Ruminococcus gnavus group—as protective against Conversely, family Ruminococcaceae, genus Flavonifractor Ruminococcaceae UCG009 associated an increased developing Bidirectional indicated that presence does not abundance these groups. Conclusion: results support relationship revealing new targets for therapeutic intervention. These insights only deepen our understanding pathophysiology but also provide theoretical basis future medical applications, highlighting value research prevention treatment strategies

Язык: Английский

Процитировано

0
The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysm DOI Creative Commons
Xuanxin Lyu,

Ming Jiang,

Jiahao Shi

и другие.

PeerJ, Год журнала: 2025, Номер 13, С. e19222 - e19222

Опубликована: Апрель 7, 2025

Background In recent years, a novel animal abdominal aortic aneurysm (AAA) model was established by administering erythropoietin (EPO) to wild-type (WT) mice. However, the influence of EPO on murine fecal microbiota remains uninvestigated. Therefore, this study aims explore potential association between gut changes and AAA development in model. Methods results Adult male C57BL/6 mice were used establish intraperitoneal administration recombinant human at dosage 10,000 IU/kg daily for 28 consecutive days. Hematoxylin eosin (H&E) Elastin Van Gieson (EVG) staining revealed that increased wall thickness diameter, accompanied enhanced degradation elastic lamina. The 16S rRNA—sequencing data deposited Sequence Read Archive (PRJNA1172300). LEfSe analysis Akkermansia, Lawsonibacter, Clostridium, Neglectibacter significantly associated with EPO-induced development, while Lactobacillus, Alistipes, Limosilactobacillus, Eisenbergiella showed significant negative correlations. Analysis using Kyoto Encyclopedia Genes Genomes (KEGG) prediction module differences metabolic pathways two groups, including alanine, aspartate glutamate metabolism; cysteine methionine pyrimidine carbon ABC transporters; oxidative phosphorylation pathways. Conclusions dysbiosis, particularly Alistipes abundance, may contribute formation via inflammation, stress, dysfunction. While advances research, its limitations underscore need validation mechanistic studies. Future work should prioritize multi-omics integration cross-model comparisons unravel complex microbiota-AAA axis.

Язык: Английский

Процитировано

0