Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles

Antibiotics, Год журнала: 2024, Номер 13(3), С. 241 - 241

Опубликована: Март 5, 2024

This study investigated the presence, distribution, and antimicrobial resistance profiles of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in a dairy herd located Northern Italy. The feces clinically healthy calves, their mothers, cows treated for mastitis, as well water, environmental samples, waste milk were collected subjected to bacteriological culture on CHROMagarTM ESBL plates. A questionnaire was administered identify risk factors. isolates identified E. by MALDI-TOF MS double-disk synergy test (DDST) minimal inhibitory concentration (MIC) assay. As result, isolated from 28 37 (75.67%) 2 3 (66.67%) cows, 8 14 (57.15%) milk. All showed multiple resistances categorized multidrug-resistant (MDR). Several factors selection diffusion identified, including lack routine cleaning calf feeding housing equipment, administration male blanket dry cow therapy. In conclusion, this highlighted presence MDR, most association with different sample sources. Accordingly, adding prudent use antibiotics, adoption adequate farm hygiene biosecurity measures might also help prevent spread transmission within herd.

Язык: Английский

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Severe aspiration pneumonia in the elderly

Journal of Intensive Medicine, Год журнала: 2024, Номер 4(3), С. 307 - 317

Опубликована: Апрель 10, 2024

The global population is aging at an unprecedented rate, resulting in a growing and vulnerable elderly need of efficient comprehensive healthcare services that include long-term care skilled nursing facilities. In this context, severe aspiration pneumonia, condition carries substantial morbidity, mortality, financial burden, especially among patients requiring admission to the intensive unit, has attracted greater concern. Aspiration pneumonia defined as pulmonary infection related or dysphagia etiology. Prior episodes coughing on food liquid intake, history relevant underlying conditions, abnormalities videofluoroscopy water swallowing, gravity-dependent shadow distribution chest imaging are clues suggest aspiration. Patients with tend be elderly, frail, suffering from more comorbidities than those without condition. Here, we comprehensively address epidemiology, clinical characteristics, diagnosis, treatment, prevention, prognosis community-acquired optimize high-risk demographic, enhance outcomes, minimize costs associated illness. Emphasizing preventive measures effective management strategies vital ensuring well-being our population.

Язык: Английский

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Risk factors associated with multidrug-resistant Klebsiella pneumoniae infections: a multicenter observational study in Lebanese hospitals

BMC Public Health, Год журнала: 2024, Номер 24(1)

Опубликована: Окт. 25, 2024

Klebsiella pneumoniae is a significant global public health burden, especially in low-income countries and regions with fragile healthcare infrastructures, due to its ability cause severe infections, increase mortality rates, rising antimicrobial resistance. This study aimed estimate the proportion of multidrug-resistant (MDR) K. infections identify associated risk factors. Data were retrospectively collected from three academic hospitals Beirut, Lebanon, between January 2021 September 2023 using standardized form. Binary logistic regression was used determine factors MDR, extended-spectrum beta-lactamase (ESBL)-producing, carbapenem-resistant (CRKP) infections. Out 2,655 cases, 410 met inclusion criteria. The primary infection sources urinary tract (58.3%) respiratory (12.4%). Among isolates, 61% MDR pneumoniae, 7.3% being extensively drug-resistant, 0.5% pandrug-resistant. Additionally, 36.8% ESBL-producing, while 6.3% CRKP. Predictors significantly included male sex (adjusted odds ratio [AOR] = 3.46, 95% CI 1.01–11.86, P 0.04), recent antibiotics use (AOR 4.52, 1.65–12.36, 0.003), cancer chemotherapy 3.43, 1.25–9.42, 0.01). ESBL-producing age ≥ 65 years, higher Charlson Comorbidity Index (CCI), antibiotic use. CRKP linked sex, prior use, longer hospital stays (all < 0.05). are steadily along an cases. main for chemotherapy. advanced age, CCI, prolonged stays. situation further exacerbated by inadequate infrastructure suboptimal national surveillance. Strengthening local surveillance implementing effective stewardship programs critical managing this growing threat..

Язык: Английский

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Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece

Foods, Год журнала: 2025, Номер 14(2), С. 224 - 224

Опубликована: Янв. 13, 2025

Extended-spectrum-β-lactamase (ESBL) and carbapenemase-producing Escherichia coli, Klebsiella pneumoniae Acinetobacter spp. are associated with hospital-acquired infections commonly isolated across the poultry food production chain. Comprehensive data regarding prevalence, spatiotemporal variations, characterization of β-lactam-resistant bacteria in farms slaughterhouses is scarce. This study examines prevalence characteristics E. K. pneumoniae, from farms, slaughterhouses, personnel Greece. Strains were selectively identified via MALDI-TOF MS, which was also employed to identify possible relatedness. coli isolates further classified into phylogenetic groups. The strains farm slaughterhouse environments 15.0% (n = 15 strains)/57.3% 71 strains) for 11.0% 11 strains)/1.6% 2 1.0% 1 strain)/25.8% 38 spp., respectively. on farmers’ skin 16.7% 8.3% strain), correspondingly. Significantly higher isolation rates observed warmer seasons. All multidrug-resistant most carried ESBL/AmpC genes. Most belonged phylogroups A (41.4%, n 36) B1 (24.1%, 21). Proteomic analysis indicated relatedness among different regions Thus, may serve as significant reservoirs

Язык: Английский

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Degradation of plasmid-mediated resistance genes in poultry slaughterhouse wastewater employing a UV/H2O2 process: A metagenomic approach

Chemosphere, Год журнала: 2025, Номер 372, С. 144109 - 144109

Опубликована: Янв. 17, 2025

Язык: Английский

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Metatranscriptomic time series insight into antibiotic resistance genes and mobile genetic elements in wastewater systems under antibiotic selective pressure

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 27, 2025

Wastewater systems are usually considered antibiotic resistance hubs connecting human society and the natural environment. Antibiotic usage can increase abundance of both ARGs (antibiotic genes) MGEs (mobile gene elements). Understanding transcriptomic profiles remains a major research goal. Batch experiments were conducted by adding ampicillin to fresh activated sludge final concentrations 100 20 mg/L. The concentration was detected via UPLC‒MS/MS. Metatranscriptomic data from samples in reactors time series (0 10 h). transcription summarized searching metatranscriptomic datasets against related databases. During incubation, dosed could be removed completely within 8.5 h 4 h, following first-order biodegradation kinetics models (R2: 0.983–0.991). phylogenetic composition ARG at DNA level remained stable. At transcriptional level, significant upregulation class A beta-lactamase (P14171 gene) observed. P14171 is mostly spread wastewater treatment carried Rhodobacteraceae. Transcription (especially TnpA) sul1 stimulated external ampicillin. However, no correlations (ρ > 0.7, p < 0.01) between across all detected. only key that responded degradation tested systems, indicating its ability potential health risk. External accelerate HGT increasing MGEs.

Язык: Английский

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Cefepime-enmetazobactam: first approved cefepime-β- lactamase inhibitor combination for multi-drug resistant Enterobacterales

Future Microbiology, Год журнала: 2025, Номер unknown, С. 1 - 10

Опубликована: Фев. 26, 2025

Cefepime-enmetazobactam is a β-lactam/β-lactamase inhibitor (BL/BLI) combination that has demonstrated potent activity against extended spectrum beta-lactamase (ESBL)-producing Enterobacterales, recently approved by the US FDA for treatment of complicated urinary tract infections (cUTI) and European Medicines Agency UK Healthcare products Regulatory cUTI, hospital-acquired pneumonia including ventilator-associated bacteremia in adults. Cefepime 4th generation cephalosporin with broad bactericidal enhanced stability to degradation chromosomal plasmid-mediated AmpC cephalosporinases as well carbapenemase OXA-48 like enzymes. Enmetazobactam novel penicillanic acid sulfone β-lactamase structurally similar tazobactam CTX-M, TEM, SHV ESBL, other class A β-lactamases, restores cefepime vitro vivo ESBLs-producing Enterobacterales. The targeted cefepime-enmetazobactam ESBLs which are resistant common antibiotics, together its superior results piperacillin/tazobactam phase 3 trial make this new BL/BLI an effective carbapenem sparing option serious caused In addition, if clinical data supportive, potential role pathogens co-producing enzymes along ESBLs.

Язык: Английский

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Drug susceptibility of uropathogens isolated from patients treated at the Mazovian Specialized Hospital in Radom

Acta Biochimica Polonica, Год журнала: 2025, Номер 72

Опубликована: Фев. 27, 2025

Urinary tract infections (UTI) are a significant problem among populations worldwide. It is mainly associated with the increasing incidence of recurrence, complications and drug resistance uropathogens. The aim this study was to demonstrate prevalence pathogens causing urinary infections. material for data obtained from Mazovian Specialized Hospital (M.S.H) in Radom over period 2 years. Urine collected hospitalized patients UTI. Statistical calculations were performed using statistical software. During period, 3,917 underwent microbiological examination urine, almost 15% them found be infected Based on analysis susceptibility most common uropathogens, it shown that caused by Escherichia coli or Klebsiella pneumoniae, others, often show high fluoroquinolones β-lactam antibiotics. Proteus mirabilis strains have been more resistant aminoglycosides than beta-lactams. In case Pseudomonas aeruginisa, predominates. On other hand, UTI Acientobacter baumannii should treated based results testing due multidrug-resistant strains.

Язык: Английский

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Detection of blaCTX-M, blaTEM, and blaSHV genes in ESBL-producing enterobacterales from poultry farms in the peri-urban area of Ouagadougou, Burkina Faso

Journal of Microbiology and Antimicrobials, Год журнала: 2025, Номер 17(1), С. 14 - 22

Опубликована: Фев. 14, 2025

Язык: Английский

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Emergence of highly virulent multidrug and extensively drug resistant Escherichia coli and Klebsiella pneumoniae in buffalo subclinical mastitis cases

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 5, 2025

This study aimed to characterize virulence and antibiotic resistance genes in multidrug-resistant (MDR) extensively drug-resistant (XDR) Escherichia coli Klebsiella pneumoniae isolated from cases of subclinical mastitis (SCM) buffaloes. A cross-sectional was conducted on 1540 quarter milk samples collected 385 Milk were screened using the California Mastitis Test Modified Whiteside Test. Positive underwent bacterial culture, biochemical tests, biofilm detection molecular analysis for pathogen identification virulence, resistance, extended-spectrum beta-lactamase (ESBL) genes. The prevalence SCM 67.9% (1046/1540) at level 80.8% (311/385) animal level. E. identified 9.5% (146/1540) samples, while K. detected 9.09% (140/1540). Virulence genes, such as stx1 (27.4%), including aac(3)-iv (77.4%) tetA (76.7%), exhibited higher prevalence. Additionally, β-lactamase notably blaTEM (67.1%), ESBL blaCTX-M1, detected. Biofilm formation 83.6% (122/146) isolates 75.7% (106/140) isolates. Antimicrobial susceptibility testing revealed significant ampicillin, amoxicillin-clavulanic acid, aminoglycosides. MDR observed 31.5% 39.3% isolates, with XDR rates 8.9% 12.9%, respectively. These findings underscore alarming spread resistant pathogens SCM-affected buffaloes, emphasizing urgent need ongoing surveillance targeted intervention strategies.

Язык: Английский

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