BMC Cardiovascular Disorders, Год журнала: 2024, Номер 24(1)
Опубликована: Дек. 30, 2024
To screen Myocardial ischemia-reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI.
Язык: Английский
Pharmacological Research, Год журнала: 2025, Номер 212, С. 107596 - 107596
Опубликована: Янв. 10, 2025
Sepsis is a life-threatening syndrome characterized by organ dysfunction caused dysregulated host response to infection. Sepsis-associated acute liver injury (SA-ALI) frequent and serious complication of sepsis that considerably impacts both short-term long-term survival outcomes. In intensive care units (ICUs), the mortality rate patients with SA-ALI remains high, mostly due absence effective early diagnostic markers suitable therapeutic strategies. Recent studies have demonstrated importance non-coding RNAs (ncRNAs) in development progression SA-ALI. This review focuses on critical roles ncRNAs, including microRNAs (miRNAs), long (lncRNAs), circular (circRNAs), regulating "cytokine storms", oxidative stress, mitochondrial dysfunction, programmed cell death SA-ALI, summarizes current state limitations existing lncRNAs circRNAs By integrating advancements high-throughput sequencing technologies, this provides novel insights into dual potential ncRNAs as biomarkers targets, offers new ideas for diagnosis treatment research highlights challenges clinical translation.
Язык: Английский
Процитировано
1
Circulation, Год журнала: 2025, Номер unknown
Опубликована: Март 18, 2025
BACKGROUND: Phenotypic switching of vascular smooth muscle cells (VSMCs), leading to neointimal hyperplasia, is a fundamental cause remodeling diseases such as atherosclerosis and hypertension. Novel hidden proteins encoded by circular RNAs play crucial roles in disease progression, yet their involvement has not been comprehensively studied. This study identifies novel protein derived from RNA VSMCs demonstrates its potential role regulating remodeling. METHODS: Cell proliferation assays were performed investigate the effects circSETD2(14,15 ) on VSMC proliferation. Techniques vector construction, immunoprecipitation–mass spectrometry, dual-luciferase reporter gene used confirm that protein, p-414aa. The interaction between p-414aa HuR (human antigen R) was validated with techniques coimmunoprecipitation, mass proximity ligation assay. Through experiments including sequencing immunoprecipitation, C-FOS (C-Fos proto-oncogene) mRNA revealed. hyperplasia assessed carotid artery model male mice. RESULTS: Overexpression inhibits phenotypic switching. p-414aa, ), interacts reduce stability, thereby suppressing ultimately inhibiting CONCLUSIONS: We uncover called CircSETD2(14,15 may serve therapeutic targets for diseases.
Язык: Английский
Процитировано
0
Frontiers in Pharmacology, Год журнала: 2025, Номер 15
Опубликована: Янв. 7, 2025
Prostate cancer (PC) is the most frequently diagnosed in men and continues to be a major cause of cancer-related mortality worldwide. In recent years, non-coding RNAs (ncRNAs) have emerged as significant focus molecular biology research, playing pivotal role development progression PC. This study employed bibliometric analysis explore global outputs, research hotspots, future trends ncRNA-related PC over past 20 years. Publications on PC-related ncRNAs from 2004 2023 were retrieved Web Science Core Collection. The co-operation network countries, institutions, authors this topic was analyzed using CiteSpace (version 6.2. R6). addition, co-occurrence keywords co-citation references performed CiteSpace, emergent detection also performed. A total 2,951 articles finally included for analysis. China contributed largest number publications, while United States influential country field, with collaborative ties 26 other countries. Fudan University identified active institution field. Rajvir Dahiya prolific author. Within network, clusters labeled "EVs," "circRNA," "ceRNA" represented current directions. cluster "gene" dominated keywords. "circRNA" showed highest burst strength among keywords, "EVs" "exosome" maintaining sustained strength, suggesting these are emerging frontiers Investigating subjects essential addressing public health impact disease advancing innovative diagnostic targeted therapeutic strategies. provides comprehensive related PC-associated ncRNAs, delivering scientific perspective identifying potential directions scholars
Язык: Английский
Процитировано
0
International Journal of General Medicine, Год журнала: 2025, Номер Volume 18, С. 209 - 219
Опубликована: Янв. 1, 2025
Background: This study analyzed the expression and diagnostic value of hsa_circ_101209 in plasma pregnant women with deep vein thrombosis (DVT).Methods: By circRNA microarray detection GO/KEGG analysis, hsa_circ_14797 targeting miRNA-mRNA network was predicted.Sixty DVT were selected as group, 60 without non-DVT group.hsa_circ_14797 detected, well D-dimer (D-D) concentration P-selectin expression.Target genes that may be regulated by predicted, GO analysis KEGG pathway enrichment performed.Results: highly expressed DVT.hsa_circ_14797 patients positively correlated D-D (r = 0.358, P 0.001).The AUC hsa_circ_14797, D-D, for maternal diagnosis 0.787 (95% CI: 0.710-0.864),0.882 0.821-0.943),and 0.825 0.754-0.895),respectively.The combined 0.886 0.828-0.944).The 0.904 0.853-0.954).The 0.935 0.893-0.978).The 0.953 0.920-0.986).The functions included biological processes angiogenesis, vascular development, morphology.The pathways PI3K-Akt pathway, TGF-β cytokine-cytokine receptor interaction.Conclusion: are increased, is D-D.hsa_circ_14797 can improve accuracy contribute to early DVT.
Язык: Английский
Процитировано
0
Oncology Reports, Год журнала: 2025, Номер 53(3)
Опубликована: Янв. 21, 2025
Язык: Английский
Процитировано
0
Cellular Signalling, Год журнала: 2025, Номер unknown, С. 111758 - 111758
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)
Опубликована: Март 26, 2025
Alternative splicing not only expands the genetic encoding of genes but also determines cellular activities. This study aimed to elucidate regulation mechanism and biological functions lincRNA-ASAO in process odontogenesis-related alternative mediated odontogenic differentiation hDPSCs. RACE, RNA-seq, FISH bioinformatics techniques were used identify novel lincRNA-ASAO. ALP staining, alizarin red qRT-PCR western blot role regulating odontoblast The binding protein PTBP1 was screened by RNA-Pulldown, profiling bioinformatics. target gene ALPL lincRNA-ASAO/PTBP1 identified technology DNA agarose gel electrophoresis. FISH, IF, PAR-CLIP determine roles lincRNA-ASAO, pre-mRNA We a that could promote human Dental Pulp Stem Cells (hDPSCs). And, interaction between factor promoted In addition, forms duplexes with pre-mRNA, targeting exonic silencer (ESS) exon 2 skipping. Notably, regulated production increase type splice variant, which have can found skipping resulting variant ALPL. Our results enrich understanding lncRNAs hDPSCs, provide clues improve clinical therapeutic potential hDPSCs for dental pulp restoration.
Язык: Английский
Процитировано
0
Cell Regeneration, Год журнала: 2025, Номер 14(1)
Опубликована: Март 26, 2025
Abstract Accumulated studies have demonstrated that long noncoding RNAs (lncRNAs) play crucial regulatory roles in diverse biological processes, such as embryonic development and cell differentiation. Comprehensive transcriptome analysis identifies extensive lncRNAs, gradually elucidating their functions across various contexts. Recent highlighted the essential role of lncRNAs definitive endoderm differentiation, underscoring importance early development. In this review, we analyzed features overlapping, proximal, desert classified by genomic location, pluripotent stem cells (PSCs) differentiation derivatives. Furthermore, focus on lineage review latest advancements lncRNA identification distinct mechanisms. By consolidating current knowledge, aim to provide a clearer perspective how contribute different manners.
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Март 27, 2025
Abstract This study aimed to investigate the involvement of hsa_circ_0074763 in activation HSCs (hepatic stellate cells ) and liver fibrosis. Additionally, it conduct a preliminary analysis molecular mechanism targeting miR-3667-3p/ACSL4 (Long-chain acyl-CoA synthetase 4), thereby providing novel targets for The GEO database was utilized identify differentially expressed determined its subcellular localization LX-2 using fluorescence situ hybridization. Bioinformatics employed result prediction, interaction between miR-3667-3P confirmed dual-luciferase reporter gene assay. ACSL4 mediated ferroptosis detected with kit. Hsa_circ_0074763 exhibits high expression levels fibrosis model. Validation through assays confirms miR-3667-3P. Functional cell experiments demonstrate that overexpression promotes proliferation cells, elevates inflammation levels, inhibits apoptosis. has been identified as direct target miR-3667-3P, counteracting inhibitory effect on by suppressing Overexpression increased ROS (Lipid Oxidation), Iron (Ferro Orange) MDA (Malondialdehyde), decreased GPX4 (Glutathione peroxidase 4) GSH (Glutathione). Our finding suggests likely enhances HSC modulation miR-3667-3P/ACSL4 axis. Therefore, holds potential therapeutic
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0