Exosomal communication: a pivotal regulator of bone homeostasis and a potential therapeutic target DOI Creative Commons

Qian-Yun Ye,

Yan Cui, Haoyu Wang

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Дек. 23, 2024

Bone homeostasis encompasses two interrelated aspects: bone remodeling and cartilage metabolism. Disruption of can lead to the development metabolic diseases such as osteoporosis osteoarthritis. The maintenance is a complex process that does not solely rely on functions tissue itself. In fact, an isolated entity; it closely connected other tissues in body via exosomes. Within this interconnectivity, exosomes derived from both non-bone cells interfere with each other, forming regulatory network. Therefore, cell origin guiding principle, we have delineated network exosomes, elaborated specific roles mechanisms common types (cells within skeletal microenvironment, stem extra-osseous tissues, vascular-derived cells, muscle-derived neurogenic cells) formation, resorption, We also discussed challenges faced field exosome research related homeostasis, unveiled critical role maintaining proposed could serve highly valuable therapeutic targets for diseases.

Язык: Английский

Icariin modulates osteogenic and adipogenic differentiation in ADSCs via the Hippo-YAP/TAZ pathway: a novel therapeutic strategy for osteoporosis DOI Creative Commons

Shaozi Lin,

Zuyu Meng,

Mei Wang

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 15

Опубликована: Янв. 13, 2025

Adipose-derived stem cell (ADSC) transplantation presents a promising approach for osteoporosis (OP) treatment. However, the therapeutic efficacy of ADSCs is hindered by low post-transplantation survival rates and limited capacities adhesion, migration, differentiation. Icariin (ICA), primary active compound Epimedium, has been shown to promote proliferation induce osteogenic differentiation; however, its specific effects on ADSC osteogenesis mechanisms which ICA enhances treatment through remain inadequately understood. This study investigates different concentrations adipogenic differentiation rat ADSCs, aiming elucidate underlying mechanisms. were isolated from female SPF-grade SD rats, with surface markers identified flow cytometry. Osteogenic assessed using Alizarin Red Oil O staining, respectively. Third-generation divided into five groups: control, resveratrol (100 μmol/L), four groups (1, 10, 50, 100 μmol/L). Western blotting was performed analyze expression factors associated Hippo-YAP/TAZ signaling pathway marker PPARγ. Additionally, labeled lentiviruses carrying enhanced green fluorescent protein (EGFP) 5-bromo-2-deoxyuridine (BrdU) assess their in vivo distribution, survival, proliferation, post-ICA intervention. In vitro, significantly inhibited Hippo pathway, reducing YAP TAZ phosphorylation enhancing transcriptional activity, while simultaneously suppressing promoted adipogenesis ADSCs. vivo, ICA-treated demonstrated effective following subcutaneous injection allogeneic rats. Our demonstrates that inhibiting adipogenesis, providing novel insights strategies related conditions.

Язык: Английский

Процитировано

0
Advanced Carriers for Precise Delivery and Therapeutic Mechanisms of Traditional Chinese Medicines: Integrating Spatial Multi‐Omics and Delivery Visualization DOI Open Access
Yusheng Zhang, Zhiguo Lü, Jing Guo

и другие.

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 19, 2025

Abstract The complex composition of traditional Chinese medicines (TCMs) has posed challenges for in‐depth study and global application, despite their abundance bioactive compounds that make them valuable resources disease treatment. To overcome these obstacles, it is essential to modernize TCMs by focusing on precise This involves elucidating the structure‐activity relationships within compositions, ensuring accurate in vivo delivery, monitoring delivery process. review discusses research progress precision treatment from three perspectives: spatial multi‐omics technology therapeutic activity, carrier systems medical imaging visualizing aim establish a novel paradigm advances therapy TCMs.

Язык: Английский

Процитировано

0
NIR-Driven PdH Nanocubes Releasing Hydrogen to Reprogram Inflammatory Microenvironment for Immune Tolerance in Therapy of Rheumatoid Arthritis DOI
Zheng Li, Min Tian,

Yimin Du

и другие.

Composites Part B Engineering, Год журнала: 2025, Номер unknown, С. 112203 - 112203

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Engineered extracellular vesicles: an emerging nanomedicine therapeutic platform DOI

Jingshi Tang,

Dezhong Li, Rui Wang

и другие.

Chemical Communications, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Extracellular vesicles have been deemed as potential drug carriers for treatment of various diseases. Recent advances summarized, including the sources, delivery function, extraction and cargo-loading technology extracellular vesicles.

Язык: Английский

Процитировано

0
Cytokine mRNA Delivery and Local Immunomodulation in the Placenta using Lipid Nanoparticles DOI Open Access

Samuel Hofbauer,

J. Lynn Fink, Rachel E. Young

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 8, 2025

Abstract During pregnancy, the maternal immune system adapts to balance tolerance of semi-allogenic fetus while protecting from pathogens. Dysregulated activity at maternal-fetal interface contributes pregnancy complications, such as recurrent loss and preeclampsia. Compared healthy placentas, preeclamptic placentas exhibit increased pro-inflammatory signaling, including a predominance inflammatory macrophages, leading impaired tissue remodeling restricted blood flow. However, precise mechanisms driving this imbalance remain poorly understood, in part due lack tools probe individual pathways. Here, we use lipid nanoparticles (LNPs) deliver cytokine-encoded mRNA placental cells, called trophoblasts, enabling local immunomodulation. LNP-mediated delivery IL-4 IL-13 induced cytokine secretion by polarization primary human monocytes toward anti-inflammatory phenotypes. Notably, lowering dose expression alternatively-activated macrophage markers, revealing an inverse relationship between status. Intravenous injection LNPs pregnant mice achieved with minimal changes cytokines serum. These findings establish tool for immunomodulation placenta, offering strategy study treat dysfunction other conditions.

Язык: Английский

Процитировано

0
Mesenchymal Stromal Cell-Based Therapy: A Promising Approach for Autoimmune Diseases DOI
Liming Li, Yong He,

Junpeng Zhao

и другие.

Clinical Reviews in Allergy & Immunology, Год журнала: 2025, Номер 68(1)

Опубликована: Фев. 21, 2025

Язык: Английский

Процитировано

0
Extracellular vesicles as therapeutic agents in rheumatoid arthritis: a systematic review of current evidence DOI
Xiaolei Miao,

Amirreza Ghafourian,

Mahdi Karimi Khaneghah

и другие.

Inflammopharmacology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Tetrahedral DNA Nanoframework‐Based Multivalent Aptamers Functionalized Biomimetic Hydrogel Scaffold Enhances Osteochondral Regeneration by Recruitment and Protection of Endogenous Stem Cells DOI
Fei Gao, Qiuyun Zhang, Yi Wang

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 25, 2025

Abstract The recruitment of endogenous stem cells is crucial for in‐situ tissue regeneration, especially addressing the limited self‐repair capacity bone and cartilage. However, recruited are vulnerable to damage from oxidative stress, leading a loss differentiation potential. Here, well‐designed strategy presented osteochondral defect repair that enhances cell recruitment, protection, differentiation. Multivalent DNA aptamers created by conjugating tetrahedral with cell‐specific aptamer, Apt19s, improve binding migration in vivo. Additionally, icariin epigallocatechin gallate respectively inserted into double strands PolyApt, resulting ATI ATE, which effectively scavenge reactive oxygen species, protect promote chondrocytes osteoblasts. Furthermore, degradable gradient hydrogel scaffold based on gelatin methacryloyl poly(ethylene glycol) diacrylate fabricated, upper layers ATE bottom layers, provide early mechanical support enhance regeneration upon degradation. Overall, approach recruiting, protecting, guiding specific represents universal offering significant potential clinical translation.

Язык: Английский

Процитировано

0
Mesenchymal stem cells arouse myocardial NAD+ metabolism to alleviate microgravity-induced cardiac dysfunction DOI Creative Commons
Chuanjie Zhang,

Yanhong Yuan,

Shuhui Zhang

и другие.

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 733, С. 150623 - 150623

Опубликована: Сен. 2, 2024

After prolonged space operations, astronauts showed maladaptive atrophy within mostly left-ventricular myocardium, resulting in cardiac dysfunction. However, the mechanism of dysfunction under microgravity conditions is unclear, and relevant prevention treatment measures also need to be explored. Through simulating environment with a tail suspension (TS) model, we found that long-term exposure promotes aging mouse hearts, which closely related The intravenous administration adipose-derived mesenchymal stem cells (ADSCs) emerged preventive therapeutic effect against myocardial senescence decline function. Plasma metabolomics analysis suggests loss NAD+ TS mice motivated NAD + metabolism utilization ADSCs-treated mice, likely accounting for ADSCs' Oral nicotinamide mononucleotide (NMN, precursor) similar ADSCs treatment. Collectively, these data implicate microgravity-induced provide new ideas aging-related remodeling.

Язык: Английский

Процитировано

0
Extracellular vesicles: immunomodulation, diagnosis, and promising therapeutic roles for rheumatoid arthritis DOI Creative Commons
Desalegn Abebaw, Yibeltal Akelew, Adane Adugna

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 18, 2024

Extracellular vesicles (EV) can be produced as part of pathology and physiology with increased amounts in pathological conditions. EVs carry transfer cargo such proteins, nucleic acids, lipids to target cells mediate intercellular communication resulting modulation gene expression, signaling pathways, phenotype recipient cells. greatly influence the extracellular environment immune response. Their immunomodulatory properties are crucial rheumatoid arthritis (RA), a condition marked by dysregulated modulate functions innate adaptive RA pathogenesis. Differentially expressed EV-associated molecules RA, microRNAs (miRNAs), long-noncoding RNAs (lncRNAs), messenger (mRNAs) proteins promising markers diagnose disease. miRNA, lncRNA, circular RNA (circRNA) cargos EV regulate inflammation pathogenic fibroblast-like synoviocytes (RA-FLS). Downregulated tissue drugs encapsulated for therapy. This review provides an updated overview EVs’ immunomodulatory, diagnostic, therapeutic roles, particularly emphasizing mesenchymal stem cell-derived (MSC-EVs).

Язык: Английский

Процитировано

0